4,252 research outputs found
Recommended from our members
Acoustic scattering by near-surface inhomogeneities in porous media.
A theoretical and experimental investigation into the influence of nearsurface ihhomogeneities on the reflection of air-borne acoustic fields at a porous ground surface is conducted. Two theoretical approaches to the three-dimensional physical problem are presented, both being initially formulated as boundary value problems but with subsequent reformulation as boundary integral equations via Green’s Second Theorem. In the first near-surface inhomogeneity approach, a rigid inhomogeneity is embedded within the porous medium and the boundary value problem is formulated by assuming continuity of pressure and normal velocity at the ground surface, Sommerfeld’s radiation conditions, and the Neumann boundary condition on the surface of the inhomogeneity. In the second surface inhomogeneity approach, the boundary value problem is formulated by assuming an impedance boundary condition on the plane boundary. Any near-surface inhomogeneities are assumed to induce a local variation of surface impedance within the boundary, and analytical expressions for such induced variations in surface impedance are presented. The resultant integral equations require knowledge of the Green’s function for acoustic propagation in the presence of a plane boundary but in the absence of the inhomogeneity, and methods for calculating these Green’s functions are discussed. The numerical solution of the boundary integral equations by a simple , boundary element method is described. The solution, which reduces to a system of linear equations with a block circulant coefficient matrix, is applicable to any inhomogeneity which is axisymmetric about a vertical axis; and for the near-surface inhomogeneity approach, the inhomogeneity must also be smooth. The numerical solutions have shown good agreement with classical results. The experimental measurements, presented in the form of spectra of the difference in sound pressure levels received at vertically separated points above surfaces of different media containing various scatterers, are in good agreement with the theoretical predictions
Comparison of stainless and mild steel welding fumes in generation of reactive oxygen species
<p>Abstract</p> <p>Background</p> <p>Welding fumes consist of a wide range of complex metal oxide particles which can be deposited in all regions of the respiratory tract. The welding aerosol is not homogeneous and is generated mostly from the electrode/wire. Over 390,000 welders were reported in the U.S. in 2008 while over 1 million full-time welders were working worldwide. Many health effects are presently under investigation from exposure to welding fumes. Welding fume pulmonary effects have been associated with bronchitis, metal fume fever, cancer and functional changes in the lung. Our investigation focused on the generation of free radicals and reactive oxygen species from stainless and mild steel welding fumes generated by a gas metal arc robotic welder. An inhalation exposure chamber located at NIOSH was used to collect the welding fume particles.</p> <p>Results</p> <p>Our results show that hydroxyl radicals (<sup>.</sup>OH) were generated from reactions with H<sub>2</sub>O<sub>2 </sub>and after exposure to cells. Catalase reduced the generation of <b><sup>.</sup></b>OH from exposed cells indicating the involvement of H<sub>2</sub>O<sub>2</sub>. The welding fume suspension also showed the ability to cause lipid peroxidation, effect O<sub>2 </sub>consumption, induce H<sub>2</sub>O<sub>2 </sub>generation in cells, and cause DNA damage.</p> <p>Conclusion</p> <p>Increase in oxidative damage observed in the cellular exposures correlated well with <b><sup>.</sup></b>OH generation in size and type of welding fumes, indicating the influence of metal type and transition state on radical production as well as associated damage. Our results demonstrate that both types of welding fumes are able to generate ROS and ROS-related damage over a range of particle sizes; however, the stainless steel fumes consistently showed a significantly higher reactivity and radical generation capacity. The chemical composition of the steel had a significant impact on the ROS generation capacity with the stainless steel containing Cr and Ni causing more damage than the mild steel. Our results suggest that welding fumes may cause acute lung injury. Since type of fume generated, particle size, and elapsed time after generation of the welding exposure are significant factors in radical generation and particle deposition these factors should be considered when developing protective strategies.</p
A Small Footprint, High Capacity Flow Reactor for UV Photochemical Synthesis on the Kilogram Scale
Structure of Tagatose-1,6-bisphosphate Aldolase. Insight into chiral discrimination, mechanism, and specificity of class II aldolases
Tagatose-1,6-bisphosphate aldolase (TBPA) is a tetrameric class II aldolase that catalyzes the reversible condensation of dihydroxyacetone phosphate with glyceraldehyde 3-phosphate to produce tagatose 1,6-bisphosphate. The high resolution (1.45 Å) crystal structure of the Escherichia coli enzyme, encoded by the agaY gene, complexed with phosphoglycolohydroxamate (PGH) has been determined. Two subunits comprise the asymmetric unit, and a crystallographic 2-fold axis generates the functional tetramer. A complex network of hydrogen bonds position side chains in the active site that is occupied by two cations. An unusual Na(+) binding site is created using a interaction with Tyr(183) in addition to five oxygen ligands. The catalytic Zn(2+) is five-coordinate using three histidine nitrogens and two PGH oxygens. Comparisons of TBPA with the related fructose-1,6-bisphosphate aldolase (FBPA) identifies common features with implications for the mechanism. Because the major product of the condensation catalyzed by the enzymes differs in the chirality at a single position, models of FBPA and TBPA with their cognate bisphosphate products provide insight into chiral discrimination by these aldolases. The TBPA active site is more open on one side than FBPA, and this contributes to a less specific enzyme. The availability of more space and a wider range of aldehyde partners used by TBPA together with the highly specific nature of FBPA suggest that TBPA might be a preferred enzyme to modify for use in biotransformation chemistry
A versatile reducible polycation-based system for efficient delivery of a broad range of nucleic acids
Synthetic vectors based on reducible polycations consisting of histidine and polylysine residues (HIS RPCs) were evaluated for their ability to deliver nucleic acids. Initial experiments showed that RPC-based vectors with at least 70% histidine content mediated efficient levels of gene transfer without requirement for the endosomolytic agent chloroquine. Significant gene transfer was observed in a range of cell types achieving up to a 5-fold increase in the percentage of transfected cells compared to 25 kDa PEI, a gold standard synthetic vector. In contrast to 25 kDa PEI, HIS RPCs also mediated efficient transfer of other nucleic acids, including mRNA encoding green fluorescent protein in PC-3 cells and siRNA directed against the neurotrophin receptor p75(NTR) in post-mitotic cultures of rat dorsal root ganglion cell neurons. Experiments to elevate intracellular glutathione and linear profiling of cell images captured by multiphoton fluorescent microscopy highlighted that parameters such as the molecular weight and rate of cleavage of HIS RPCs were important factors in determining transfection activity. Altogether, these results demonstrate that HIS RPCs represent a novel and versatile type of vector that can be used for efficient cytoplasmic delivery of a broad range of nucleic acids. This should enable different or a combination of therapeutic strategies to be evaluated using a single type of polycation-based vector
A Comprehensive Survey of Data Mining-based Fraud Detection Research
This survey paper categorises, compares, and summarises from almost all
published technical and review articles in automated fraud detection within the
last 10 years. It defines the professional fraudster, formalises the main types
and subtypes of known fraud, and presents the nature of data evidence collected
within affected industries. Within the business context of mining the data to
achieve higher cost savings, this research presents methods and techniques
together with their problems. Compared to all related reviews on fraud
detection, this survey covers much more technical articles and is the only one,
to the best of our knowledge, which proposes alternative data and solutions
from related domains.Comment: 14 page
Introduction: Working with the Grain? The Africa Power and Politics Programme
At the heart of current policy thinking about Africa there is a significant knowledge gap concerning governance and development. This IDS Bulletin is concerned with what can be done about that, drawing on the initial experience of a new research venture, the Africa Power and Politics Programme. The APPP is committed to discovering forms of governance that work better for development than those prescribed by the current ‘good governance’ orthodoxy. It aims to do so chiefly by examining the range of post?colonial experience in sub?Saharan Africa focusing especially on under?appreciated patterns of difference in institutions and outcomes. This article explains the rationale of this approach and outlines the options which have shaped the programme's methods and concepts. A central challenge has been operationalising the working hypothesis that institutions function better when they ‘work with the grain’ of the society which hosts them
Carbon nanotube dosimetry: from workplace exposure assessment to inhalation toxicology
BACKGROUND: Dosimetry for toxicology studies involving carbon nanotubes (CNT) is challenging because of a lack of detailed occupational exposure assessments. Therefore, exposure assessment findings, measuring the mass concentration of elemental carbon from personal breathing zone (PBZ) samples, from 8 U.S.-based multi-walled CNT (MWCNT) manufacturers and users were extrapolated to results of an inhalation study in mice. RESULTS: Upon analysis, an inhalable elemental carbon mass concentration arithmetic mean of 10.6 μg/m(3) (geometric mean 4.21 μg/m(3)) was found among workers exposed to MWCNT. The concentration equates to a deposited dose of approximately 4.07 μg/d in a human, equivalent to 2 ng/d in the mouse. For MWCNT inhalation, mice were exposed for 19 d with daily depositions of 1970 ng (equivalent to 1000 d of a human exposure; cumulative 76 yr), 197 ng (100 d; 7.6 yr), and 19.7 ng (10 d; 0.76 yr) and harvested at 0, 3, 28, and 84 d post-exposure to assess pulmonary toxicity. The high dose showed cytotoxicity and inflammation that persisted through 84 d after exposure. The middle dose had no polymorphonuclear cell influx with transient cytotoxicity. The low dose was associated with a low grade inflammatory response measured by changes in mRNA expression. Increased inflammatory proteins were present in the lavage fluid at the high and middle dose through 28 d post-exposure. Pathology, including epithelial hyperplasia and peribronchiolar inflammation, was only noted at the high dose. CONCLUSION: These findings showed a limited pulmonary inflammatory potential of MWCNT at levels corresponding to the average inhalable elemental carbon concentrations observed in U.S.-based CNT facilities and estimates suggest considerable years of exposure are necessary for significant pathology to occur at that level
Maximally-localized generalized Wannier functions for composite energy bands
We discuss a method for determining the optimally-localized set of
generalized Wannier functions associated with a set of Bloch bands in a
crystalline solid. By ``generalized Wannier functions'' we mean a set of
localized orthonormal orbitals spanning the same space as the specified set of
Bloch bands. Although we minimize a functional that represents the total spread
sum_n [ _n - _n^2 ] of the Wannier functions in real space, our method
proceeds directly from the Bloch functions as represented on a mesh of
k-points, and carries out the minimization in a space of unitary matrices
U_mn^k describing the rotation among the Bloch bands at each k-point. The
method is thus suitable for use in connection with conventional
electronic-structure codes. The procedure also returns the total electric
polarization as well as the location of each Wannier center. Sample results for
Si, GaAs, molecular C2H4, and LiCl will be presented.Comment: 22 pages, two-column style with 4 postscript figures embedded. Uses
REVTEX and epsf macros. Also available at
http://www.physics.rutgers.edu/~dhv/preprints/index.html#nm_wan
- …