Ethanol Induced Toxicity and Lipid Peroxidation in Pregnant Mice: Protective Effects of Butanolic Extract from Leaves of Chrysanthemum fontanesii, Vitamin E and C

Background: The objective of the present study was to investigate the ability of butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C to modulate ethanol-Induced toxicity and oxidation damage in maternal and fetal tissues of mice. Butanolic extract from leaves of Chrysanthemum fontanesii (200 mg/Kg per day), vitamin E (100mg/Kg per day) and C (8.3mg/Kg per day) were administered by gavage to groups of pregnant mice from the 6 th to 17 th day of gestation. A number of animals received plant extract, vitamin E and C, also treated with an oral administration of ethanol (0.02ml/g of 25% v/v absolute ethanol in water per day) in same conditions. On day 18 of gestation, pregnant mice were killed, fetus, placenta, fetal liver, liver, kidneys and brain were removed, homogenised and used for determination of lipid peroxidation (LPO) using TBARS method. Embryotoxicity was assessed by counting the number of live and dead fetus and growth retardation. Results: Severe alterations in all biomarkers were observed after injury with ETOH. ETOH produced significant decreases in fetal weight and significant increases in embryolethality and lipid peroxidation relative to control values. Treatment with Chrysanthemum fontanesii extract, vitamin C and vitamin E resulted in markedly decreased embryolethality and fetal growth retardation, while increased fetal weight were observed. Conclusion: The butanolic extract from leaves of Chrysanthemum fontanesii, vitamin E and C protected against ethanol induce fetal and maternal toxicity as revealed by the decrease in the extent of lipid peroxidation. So that butanolic extract from leaves of Chrysanthemum fontanesii posses in vivo antioxidant properties

Antioxidant activity and chemical constituents of Anthriscus vulgaris Bernh. (Apiaceae) from Algeria

AbstractThe chloroform and ethyl acetate extracts obtained from the aerial parts of Anthriscus vulgaris Bernh. were analyzed by gas chromatography-mass spectrometry (GC-MS). 36 components have been identified in each extract. The major constituents were 1-monooleoylglycerol (20.72%), caffeic acid (15.20%), cinnamic acid (11.31%) and benzene acetic acid (10.95%). The phytochemical study led to the isolation and structural elucidation of three compounds, scopoletin, umckalin and 1-(3',4'-dihydroxycinnamoyl) cyclopentane-2,3-diol. Moreover the ethyl acetate extract was screened for its possible in vitro antioxidant activity by 2,2-diphenyl-1-picrylhydrazy l(DPPH) and lipid peroxidation inhibition assays in which it displayed a noticeable activity. This study provides the first biological and chemical investigation on Anthriscus vulgaris Bernh. in Algeria

In vitro antioxidant, antibacterial and membrane stabilizing activity of plant extract from Chrysanthemum fontanesii

Antioxidant activities, antibacterial and membrane stabilizing activity of butanolic extract from leaves of Chrysanthemum fontanesii were investigated. The inhibition of the formation of malondialdehyde (MDA) in vitro and the scavenging of DPPH were assayed .The phenolic content of the extract was determined. The experimental results show that butanolic extract have antioxidant activity in vitro. The extracts showed a high antioxidant effect, especially scavenging of DPPH anions and inhibition of lipid peroxidation. Antioxidant activities were compared to ascorbic acid. The total content of phenolic compounds was 349 µg of gallic acid equivalents/mg extract. The butanolic extract was effective against microorganisms and against heat-as well as hypotonic solution-induced haemolysis of erytrocytes in vitro

Protective role of Limonium bonduelli extract against non-enzymatic peroxidation in brain and testes induced by iron in vitro.

Infertility and Neurodegerative diseases have been linked to oxidative stress arising from peroxidation of membrane biomolecules and high levels of iron have been reported to play an important role. The present study sought to determine the antioxidant activity and protective ability of n-butanol extract of Limonium bonduelli on lipid peroxidation induced by FeSO4 in rat brain and testes homogenates in vitro. n-butanol extract of the aerial parts (leaves and flowers) was prepared, and the ability of the extract to inhibit FeSO4 induced lipid peroxidation in isolated rat brain and testes was determined using spectrophotometric method. The study revealed that the extract inhibited malondialdehyde (MDA) production in FeSO4 induced lipid peroxidation in the brain and testes in a dose-dependent manner and the highest percentage of the inhibition was 89.80% similar to vitamin C in the same concentration (100 µg/mL) in brain and 82.33% in testes (200 µg/mL). Limonium bonduelli extract demonstrated important anti-lipid peroxidative effect, which may be useful in preventing the progress of various oxidative stress related diseases. The higher inhibitory effect of the extract could be attributed to its phytochemical content

In vitro Antiproliferative and inhibition of oxidative DNA damage activities of n-butanol extract of Limonium bonduelli from Algeria

Abstract Plants are the main sources of natural antioxidants in the form of phenolic compounds, which help human beings to deal with oxidative stress, caused by free radical damage. For this reason, the present study was carried out to evaluate the antiproliferative, antioxidant and inhibition of oxidative DNA damage activities of n-butanol extract obtained from aerial parts of Limonium bonduelli. The antioxidant potential was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and inhibition of lipid peroxidation assay. Antiproliferative activity was evaluated using xCELLigence RTCA instrument on two tumor cell lines; HT-29 (human colon adenocarcinoma) and HeLa (human cervix carcinoma). DNA damage inhibition was evaluated using photolyzing 46966 plasmid. Also, total phenolic and total flavonoid contents were determined using a spectrophotometric method. Total phenolic (343 ± 0.05 µg/mg) and flavonoid (220.5 ± 0.04 µg/mg) were indicated as gallic acid and quercetin equivalents respectively. The extract exhibited significant IC50 values in lipid peroxidation (IC50= 181.18 ± 0.65 µg/mL) and DPPH radical scavenging assays (IC50= 14.92 ± 0.032 µg/mL). The extract also partially protected 46966 plasmid DNA from free radical-mediated oxidative stress in a DNA damage inhibition assay and showed concentration-dependent antiproliferative effects. n-butanol extract of L. bonduelli is a rich source of natural antioxidants and anticancer agents

A flavonoid with high antioxidant effect from Centaurea acaulis L.

The flavonoid glycosides: patuletin 7-O-β-glucopyranoside 3, hispidulin 7-O-β-glucopyranoside 2 together with hispidulin 1, were isolated from the n-butanol soluble part of the aqueous-MeOH extract of the flowering aerial parts of C. acaulis L. (Asteraceae), an endemic species of Algeria and Tunisia. The structures were established by chemical and spectral analysis, mainly HR-ESIMS, UV and NMR experiments (COSY, NOESY, HSQC and HMBC). Patuletin 7-O-β-glucopyranoside showed a high antioxidant effect, especially scavenging of DPPH (IC50 = 4.83 µg/ml) anions and inhibition of lipid peroxidation (51.93%.). Those various antioxidant activities were compared to vitamin C (standard antioxidant)