Antibiotic resistance of gram negatif bacteria isolated from farming lands.

TEZ8034Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 2010.Kaynakça (s. 71-79) var.ix, 81 s. : res., çizelge ; 29 cm.Sunulan çalışmada tarım yapılma sıklığına göre (çok tarım, az tarım, tarım yapılmayan ve sera tarımı yapılan) dört farklı bölgeden izole edilen toplam 150 Gram (-) bakterinin antibiyotik direnç fenotipleri ve MAR indeksleri belirlenmiş ve 102 tanesinin identifikasyonu yapılmıştır. Çok tarım yapılan bölgelerden izole edilen bakterilerin antibiyotik direnç oranları %38.13, az tarım yapılan bölgede %16.83, tarım yapılmayan bölgede %26.67 ve sera tarımı yapılan bölgelerde ise %22.83 olarak bulunmuş, istatistiksel olarak yalnızca çok tarım yapılan bölgeler ile az tarım yapılan bölgeler arasında fark gözlenmiştir. Çok tarım yapılan bölgelerde en yüksek MAR indeksi 0.73 ile 8 bakteride, az tarım yapılan bölgede 0.67 ile 1 bakteride, tarım yapılmayan bölgelerde 0.67 ile 3 bakteride ve sera tarımı yapılan bölgelerde 0.73 ile 1 bakteride gözlenmiştir. Ayrıca çok tarım yapılan bölgede 29, Aztarım yapılan bölgede 18, tarım yapılmayan bölgede 16 ve sera tarımı yapılan bölgede ise 28 bakterinin birden fazla sayıda antibiyotiğe dirençli oldukları bulunmuştur.The proposed study determines the antibiotic resistance phenotypes and MAR indexes of 150 types of Gram (-) bacteria, isolated from four different areas according to their frequencies of cultivation (frequent cultivation, infrequent cultivation, uncultivated fields and greenhouse cultivation areas) and identifies 102 types. The antibiotic resistance rate of bacteria isolated from frequently cultivated fields, infrequently cultivated fields, uncultivated fields and greenhouse cultivation areas were found to be 38,13%, 16,83%, 26,64% and 22,83% respectively and a statistically significant difference was observed only between frequently cultivated fields and infrequently cultivated fields. It was observed that the highest MAR index in frequently cultivated fields was 0,73 with 8 bacteria, 0,67 in infrequently cultivated fields with 1 bacteria, 0,67 in uncultivated fields with 3 bacteria, and 0,73 in greenhouse cultivation fields with 1 bacteria. It was also found out that 29 bacteria in frequently cultivated fields, 18 bacteria from infrequently cultivated fields, 16 bacteria from uncultivated fields and 28 bacteria from greenhouse cultivation areas were resistant to more than one type of antibiotics.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: FEF2009YL13

Microbiological quality of Gölbaşı and Azaplı lakes (Adıyaman) and determination of antibiotic resistance properties of isolates.

TEZ10569Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2014.Kaynakça (s. 115-126) var.xv, 127 s. : res. (bzs. rnk.), tablo ; 29 cm.Bu çalışmada Gölbaşı ve Azaplı (Adıyaman) göllerinin mikrobiyolojik kirlilik düzeyleri, bazı fiziksel parametreleri araştırılarak, bu göllerden izole edilen bakterilerin identifikasyonları yapılarak, antibiyotik dirençlilik düzeyleri ile çoklu antibiyotik dirençlilik oranları belirlenmiştir. Bunun için, her gölde beş farklı istasyon belirlenerek 4 farklı periyotta (Aralık, Mart, Temmuz, Ekim) su örnekleri alınmıştır. Ortalama sıcaklık değerleri, pH, çözünmüş oksijen ve elektriksel iletkenlik değerlerine göre her iki gölün kıta içi su kaynakları yönetmeliğine göre yüksek kalitede olduğu belirlenmiştir. Her iki gölde de fekal streptokok varlığı belirlenerek toplam koliform sayısının Azaplı Kanal 1 ve 2 istasyonlarında yüksek olduğu (1100>kob/100mL), Gölbaşı gölünde ise örnek toplama periyotlarına bağlı olarak değiştiği saptanmıştır. Analizler sonucunda 464 izolatın 14 farklı türe ait olduğu belirlenmiş olup dominat tür Aeromonas sobria izolatlarıdır. İzolatların sefazolin, sefalotin, sefuroksim, seftizoksim, penisilin ve nitrofurantoin’e karşı dirençlerinin genel olarak yüksek olduğu (>%50) tespit edilmiştir. Gölbaşı gölündeki izolatların %89.5‘inde, Azaplı gölündeki izolatların ise %94.0’ünde çoklu antibiyotik direnci belirlenmiştir. Çoklu antibiyotik dirençliliği (ÇAD) 0.50 ile 1.00 arasında değişkenlik gösteren ve rastgele seçilen 103 bakteriden 15’inde plazmit varlığı tespit edilmiştir. Sonuçlar göstermektedir ki, Gölbaşı ve Azaplı gölleri fiziksel parametreler bakımından iyi kalitede suya sahip olduğunu ancak her iki gölünde mikrobiyolojik olarak kirli olduğunu göstermektedir.In this study, microbial pollution levels and some physical parameters of Golbasi and Azaplı Lakes (Adiyaman) were investigated, and also identified bacteria and determined the resistance levels and multiple antibiotic resistance of bacteria isolated from lakes. For this purpose, five place were determined for taking water samples each of lakes. Water samples were collected at four different periods (December, march, july, october). Average water temperature, pH, dissolved oxygen and conductivity results shown that lakes have a high water quality according to in land water resources management. The presence of fecal streptococ was determined each of lakes and total coliform levels was high in station of Azaplı water canal 1 and 2 (>1100), total coliform levels were showed differences depending on periods in Golbasi lake. Results obtained from analyses have shown that 14 different species from 464 isolates were identified. Dominat bacteria was Aeromonas sobria . It was observed CZ, KF, CXM, ZOX, P and F resistance were observed generally high levels (up to %50) in isolates. Multiple antibiotic resistance (MAR) were determined from the rate of 89.5% of isolates in Golbasi lake and 94% of isolates in Azapli lake. In all of randomly selected fifteen isolates have multiple antibiotic resistance presence of plasmid was observed. The results indicated that Golbasi and Azapli lakes are good quality in terms of physical parameters, but in microbiologically polluted significantly.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiştir. Proje No: FEF2013D6

Rapid isocratic HPLC investigation of radiochemical purity for Y-90-DOTATATE

DOTA-conjugated peptides, such as [DOTA(0), Tyr(3)]ocreotide (DOTATOC) and [DOTA(0), Tyr(3)]octreotate (DOTATATE) can be labeled with radionuclides such as Y-90, Lu-177 and In-111 at high specific activities. These radiolabelled somatostatin analogues are used for peptide receptor radionuclide therapy (PRRT). Currently, radio-high performance liquid chromatography (radio-HPLC) and radio-thin layer chromatography (radio-TLC) are the methods of choice for the analysis of the labeled compounds. In literature, radiochemical purity of the radiolabelled DOTATATE was investigated using gradient reversed-phase radio-HPLC. However, these studies indicate long retention time of the radiolabelled compound of 14.52 min. In our study, a new simple and rapid reversed-phase isocratic system enables the radiochemical purity of the radiolabelled DOTATATE within a few minutes

The Consideration of Diffusion MR Imaging, Dynamic Contrast-Enhanced MR and T2 Relaxation Time Measurements in Distinguishing of Cholesteatoma with Chronic Otitis Media

WOS: 000337898000004Purpose: The aim of our study is to investigate the contribution of DWI, DCE-MRI, ADC measurements and T2 relaxometry in diagnosis of distinguishing cholesteatoma and COM Materials and Methods: Our study is created by retrospectively reviewing MR images of a digital archive of totally 41 patients that were clinically or surgically diagnosed as COM and cholesteatoma. DWI, ADC values, T2 relaxation time, and CEP of lesions between the two groups were compared statistically. CEP was qualitatively evaluated. ROC analysis and McNemar statistics test were performed. Level of significance was determined as p0.05). When the groups were assessed according to the CEP, 13 of the COM cases showed type 1 (progressive heterogeneous enhancement), 2 cases did not show enhancement (type 3) and 6 cases showed type 4 (progressive homogeneous enhancement). Nineteen patients with cholesteatoma showed type 2 (rim-like enhancement), 1 case showed type 4. Statistical analysis of CEP revealed significant differences between types 1 and 2 (p<0.001), between types 2 and 3 (p=0.005) and between types 2 and 4 (p<0.001). Conclusion: DWI, ADC values and DCE-MRI may be beneficial in detecting cases of cholesteatoma of a high percentage

PET/CT and Bremsstrahlung Imaging After 90Y DOTANOC Therapy for Rectal Net With Liver Metastases

Peptide receptor radionuclide therapy with Lu-177 or Y-90 is promising with successful results in somatostatin receptor-positive tumors. In all radiation therapies, knowledge of the radiation dose received by the target, and other organs in the body is essential to evaluate the risks and benefits of any procedure. We report a case of liver metastases from a rectal neuroendocrine tumor, which was treated with Y-90 DOTANOC. Posttreatment whole-body planar images were acquired through Bremsstrahlung radiations of Y-90 on a gamma-camera, and thoracolumbar PET/CT images were acquired on PET

An Investigation of Multiple Antibiotic Resistance and Identification of Bacteria Isolated from Gölbaşı and Azaplı Lakes (Adıyaman)

Identification and multiple antibiotic resistances of amphicillin, chloramphenicol, streptomycin and tetracycline resistant gram-negative bacteria that isolated microorganisms from Gölbaşı and Azaplı lakes (Adiyaman) were investigated in this study. Seasonally taken isolates of totally 386 bacteria in 10 different species from 7 genera were scanned against 16 antibiotics [gentamycin, imipenem, kanamycin, chloramphenicol, meropenem, nalidixic acid, nitrofurantoin, penicillin, cephalothin, cefazolin, cefpirome, ceftizoxime, cefuroxime, streptomycin, tetracycline and trimethoprim-sulphamethoxazole (Bioanalyse)] by using the disc diffusion method to determine the prevalence of multiple antibiotic resistance. Multiple antibiotic resistance of stations showed seasonal changes between 0.29 and 0.91. In generally, multiple antibiotic resistance in Golbasi and Azapli lakes were higher than the reference value and highest multiple antibiotic resistance values were obtained at summer season (3th period). When the obtained data are considered, high multiple antibiotic resistance poses a risk in terms of public health and for economically important animals