Aej-OldNew.txt

Microsatellite alleles for a comparison over tim

Aej-PA.txt

Microsatellite alleles for fine resolution analysis across Pennsylvania (allele sizes

Aej-PA2.txt

Microsatellite alleles for fine resolution analysis across Pennsylvania (for Phylip

Biomarkers identified in advanced (10 months post infection) <i>M. paratuberculosis</i> infection.

<p>The bar graph represents log ratios of serum proteins (<i>P</i>-value<0.05, EF<2) identified in <i>M. paratuberculosis</i> infected (10 months PI) and control animals. Red bars represent log ratios of serum protein levels in <i>M. paratuberculosis</i> infected animals over time. The black bars represent the temporal trend in log ratios of the same proteins in control animals.</p

List of proteins identified in <i>M. paratuberculosis</i> infected animals at 10 months post-infection and contemporary controls relative to pre-infection.

1<p>117/115: ratio of the fold change of protein among animals at 10 months PI with <i>M. paratuberculosis</i> (117) against pre-infection (115).</p>2<p>116/114: ratio of the fold change of protein at 10 months to follow-up among contemporary controls(116) to pre-infection (114).</p>3<p><i>p</i>-value represent fold ration of the different reporter ions with a significance of <0.05.</p>4<p>Number of peptides represent those identified with 99% confidence in the reporter ion quantification.</p><p>NI: not identified.</p

Experimental design used for four iTRAQ analyses with combinations of sera from <i>M. bovis</i> and/or <i>M. paratuberculosis</i> infections in calves.

<p>Shown in each quadrant is the experimental design for either <i>M. bovis</i> or <i>M. paratuberculosis</i> infections. Pre- and post-infection time points at which sera were collected and applied in iTRAQ analyses are identified. Numbers in brackets represent the numbers of sera from each group that was pooled for analysis. Experimental design also included contemporary controls.</p

Validation of vitamin DBP in individual sera from <i>M. bovis</i> infected and control animals.

<p>Shown is a dot blot of levels of DBP in individual sera and the bar graph represents quantification of DBP in respective dots using densitometric analysis. Each spot contains 50-µg of total proteins from <i>M. bovis</i> infected and control sera. The first five lanes contain sera from control animals at first, third and fourth months. Lanes six to ten contains sera from <i>M. bovis</i> infection at 1, 3, and 4 months post infection. All sera were analyzed in duplicates.</p

List of serum proteins identified in <i>M. bovis</i> infected animals at 7 months post-infection and 10 months post-infection in <i>M. paratuberculosis</i> infection.

1<p>114/116: ratio of the fold change of proteins in <i>M. bovis</i> infected animals (7 months post-infection; 114) compared with pre-infection control animals (116).</p>2<p>117/114: ratio of the fold change of proteins in the <i>M. paratuberculosis</i> infection (10 months post-infection; 117) relative to <i>M. bovis</i> infection (7 months post-infection; 114).</p>3<p>117/116: ratio of the fold change of proteins in the <i>M. paratuberculosis</i> infection (10 months post-infection; 117) compared with pre-infection control animals (116).</p>3<p><i>p</i>-value represents fold ratios of the different reporter ions with a significance of <0.05.</p>4<p>Number of peptides represent those identified with 99% confidence in the reporter ion quantification.</p><p>NI: Not Identified.</p