<p>Shown is a dot blot of levels of DBP in individual sera and the bar graph represents quantification of DBP in respective dots using densitometric analysis. Each spot contains 50-µg of total proteins from <i>M. bovis</i> infected and control sera. The first five lanes contain sera from control animals at first, third and fourth months. Lanes six to ten contains sera from <i>M. bovis</i> infection at 1, 3, and 4 months post infection. All sera were analyzed in duplicates.</p