Etude biochimique et génétique de la diversité des protéines de réserve du triticale hexaploïde, de leur influence sur les caractéristiques technologiques et recherche de facteurs impliqués dans la dureté du grain par une analyse protéomique des protéines amphiphiles du blé tendre

CLERMONT FD-BCIU Sci.et Tech. (630142101) / SudocSudocFranceF

Proteomics analysis : an efficient approach for investigating the genetic and environmental bases of wheat quality

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Chromosome mapping and identification of amphiphilic proteins of hexaploid wheat kernels

International audienceAmphiphilic proteomic analysis was carried out on the ITMI (International Triticae Mapping Population) population resulting from a cross between 'Synthetic', i.e.: 'W7984' and 'Opata'. Out of a total of 446 spots, 170 were specific to either of the two parents, and 276 were common to both. Preliminary analysis, which was performed on 80 progenies (Amiour et al. 2002a), was completed here using a total of 101 selfed lines. Seventy two Loci of amphiphilic spots placed at LOD = 5 were conclusively assigned to15 chromosomes. Some spots mapped during the first analysis were eliminated because of the significant distortion segregation observed in the second analysis. Group-1 chromosomes had by far the greatest number of mapped spots (51). Using the Quantitative Trait Loci (QTLs) approach, analysis of the quantitative variation of each spot revealed that 96 spots out of the 170 specific ones showed at least one Protein Quantity Locus (PQL). These PQLs were distributed throughout the genome. With Matrix Laser Desorption Ionisation Time Of Flight (MALDI-TOF) spectrometry and Database interrogation, a total of 93 specific and 41 common spots were identified. This enabled us to show that the majority of these proteins are associated with membranes and/or play a role in plant defence against external invasions. Using multiple-regression analysis, other amphiphilic proteins, in addition to puroindolines, were shown to be involved in variation in kernel hardness in the ITMI population

A proteomic approach to studying plant response to crenate broomrape (Orobanche crenata) in pea (Pisum sativum)

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Mutations in DMI3 and SUNN Modify the Appressorium-Responsive Root Proteome in Arbuscular Mycorrhiza

International audienceModification of the Medicago truncatula root proteome during the early stage of arbuscular mycorrhizal symbiosis was investigated by comparing, using two-dimensional electrophoresis, the protein patterns obtained from non-inoculated roots and roots synchronized for Glomus intraradices appressorium formation. This approach was conducted in wild-type (J5), mycorrhiza-defective (TRV25, dmi3), and autoregulation-defective (TR122, sunn) M. truncatula genotypes. The groups of proteins that responded to appressorium formation were further compared between wild-type and mutant genotypes; few overlaps and major differences were recorded, demonstrating that mutations in DMI3 and SUNN modified the appressorium-responsive root proteome. Except for a chalcone reductase, none of the differentially displayed proteins that could be identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry previously was known as appressorium responsive. A DMI3-dependent increased accumulation of signal transduction-related proteins (dehydroascorbate reductase, cyclophilin, and actin depolymerization factor) was found to precede mycorrhiza establishment. Differences in the accumulation of proteins related to plant defense reactions, cytoskeleton rearrangements, and auxin signaling upon symbiont contact were recorded between wild-type and hypermycorrhizal genotypes, pointing to some putative pathways by which SUNN may regulate very early arbuscule formation

Plant mycorrhizal interaction: What can we learn from a proteomic approach?

International audienc

Contribution of proteomics to arbuscular mycorrhiza in Medicago truncatula

International audienceBecause proteins are key effectors of plant responses to environmental cues including recognition, signalling, transport and defence reactions, main interest has been paid to characterize those involved in the establishment and functioning of arbuscular mycorrhizal (AM) symbiosis. In our group, the setting up of high throughput proteomic techniques on the model species, Medicago truncatula, is providing step-by-step a large-scale analysis of AM symbiosis-related proteins. Depending on the symbiotic stage targeted and on the abundance of mycorrhizal material, different proteomic strategies that can be combined with other large-scale approaches (transcriptomic and metabolomic) will be presented. Modification of the M. truncatula root proteome during the early stage of AM symbiosis has been investigated by comparing the protein patterns obtained from non-inoculated roots and roots synchronized for appressorium formation in wild-type (J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn) genotypes. In mature mycorrhiza, sub-cellular proteomic approaches have been developed in M. truncatula to target symbiosis-related membrane proteins eligible as involved in nutrient transport and signalling between symbionts upon arbuscule formation. In addition, with the aim of determining overlaps in response to the two most commonly employed AM fungal isolates we recently launched a high throughput comparative proteomic analysis. The future release of the genome sequencing programs launched for M. truncatula and Glomus intraradices is likely to provide additional knowledge about AM symbiosis-related proteins

Contribution of proteomics to arbuscular mycorrhiza in Medicago truncatula

International audienceBecause proteins are key effectors of plant responses to environmental cues including recognition, signalling, transport and defence reactions, main interest has been paid to characterize those involved in the establishment and functioning of arbuscular mycorrhizal (AM) symbiosis. In our group, the setting up of high throughput proteomic techniques on the model species, Medicago truncatula, is providing step-by-step a large-scale analysis of AM symbiosis-related proteins. Depending on the symbiotic stage targeted and on the abundance of mycorrhizal material, different proteomic strategies that can be combined with other large-scale approaches (transcriptomic and metabolomic) will be presented. Modification of the M. truncatula root proteome during the early stage of AM symbiosis has been investigated by comparing the protein patterns obtained from non-inoculated roots and roots synchronized for appressorium formation in wild-type (J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn) genotypes. In mature mycorrhiza, sub-cellular proteomic approaches have been developed in M. truncatula to target symbiosis-related membrane proteins eligible as involved in nutrient transport and signalling between symbionts upon arbuscule formation. In addition, with the aim of determining overlaps in response to the two most commonly employed AM fungal isolates we recently launched a high throughput comparative proteomic analysis. The future release of the genome sequencing programs launched for M. truncatula and Glomus intraradices is likely to provide additional knowledge about AM symbiosis-related proteins