ISOLATION OF FLAVONOID COMPOUND FROM IRAQI AWSAJ PLANT (LYCIUM BARBARUM L.) FRUITS AND THE STUDY OF ITS ANTIBACTERIAL ACTIVITY

This study was performed to evaluate the in vitro antimicrobial activity and the flavonoid content of Iraqi awsaj plant (Lycium barbarum L.) fruits. The fruits extracts contain important amounts of flavonoids. The results obtained in the antimicrobial tests revealed that flavonoid compound was more active than alcoholic extract both for Gram-positive and Gram-negative bacterial strains. The results suggest that these species are valuable sources of flavonoids with relevant antimicrobial activities

Synthesis, characterization and evaluation of antiinflammatory properties of novel α, β-unsaturated ketones

Purpose: To prepare and characterize alicyclic aromatic chalcone derivatives, and study their antibiotic and anti-inflammatory properties.Methods: Claisen-Schmidt (aldol condensation) base-catalyzed condensation was used for preparation of chalcone derivatives (compounds I - IV), and the products were characterized using ultraviolet-visible spectroscopy (UV), FT-IR spectroscopy, proton nuclear magnetic resonance (1H-NMR), carbone 13CNMR and mass spectroscopy (MS). The antibacterial effect of the compounds was determined against Baci. cereus, Staph. Aureus, E. coli, and Pseudomonas Aeruginosa. In addition, their anti-inflammatory effects were assayed using cotton granule-induced granuloma in mice. The results were compared with those for diclofenac, a standard drug. The synthesized derivatives were subjected to theoretical studies on their stabilities, and some chemical parameters were calculated using density function theory [DFT].Results: Using Claisen-Schmidt reaction, it was possible to prepare stable chalcone derivatives, such as derivatives of 2-(3-phenyl  acryloyl)cyclopentan-1-one, with good results. Depending on the substituted group, it was also shown that the derivatives had effective biological effects. Compound IV displayed a noticeable antibacterial effect against Staph. aureus and E. coli. The prepared chalcone derivatives exerted markedly variable anti-inflammatory effects.Conclusion: These results indicate that Claisen-Schmidt reaction is not limited to the preparation of chalcone derivatives from diphenyl structures  only. Stable alicyclic aromatic structures can also be used. This results in derivatives with good biological effects. Keywords: Chalcones, Claisen-Schmidt, Anti-inflammatory effects, Granuloma, Aldol Condensatio

ISOLATION OF FLAVONOID COMPOUND FROM IRAQI AWSAJ PLANT (LYCIUM BARBARUM L.) FRUITS AND THE STUDY OF ITS ANTIBACTERIAL ACTIVITY

This study was performed to evaluate the in vitro antimicrobial activity and the flavonoid content of Iraqi awsaj plant (Lycium barbarum L.) fruits. The fruits extracts contain important amounts of flavonoids. The results obtained in the antimicrobial tests revealed that flavonoid compound was more active than alcoholic extract both for Gram-positive and Gram-negative bacterial strains. The results suggest that these species are valuable sources of flavonoids with relevant antimicrobial activities