Microglia maintain structural integrity during fetal brain morphogenesis

Microglia (MG), the brain-resident macrophages, play major roles in health and disease via a diversity of cellular states. While embryonic MG display a large heterogeneity of cellular distribution and transcriptomic states, their functions remain poorly characterized. Here, we uncovered a role for MG in the maintenance of structural integrity at two fetal cortical boundaries. At these boundaries between structures that grow in distinct directions, embryonic MG accumulate, display a state resembling post-natal axon-tract-associated microglia (ATM) and prevent the progression of microcavities into large cavitary lesions, in part via a mechanism involving the ATM-factor Spp1. MG and Spp1 furthermore contribute to the rapid repair of lesions, collectively highlighting protective functions that preserve the fetal brain from physiological morphogenetic stress and injury. Our study thus highlights key major roles for embryonic MG and Spp1 in maintaining structural integrity during morphogenesis, with major implications for our understanding of MG functions and brain development.</p

Verification of measured PV energy yield versus forecast and loss analysis

Yield forecasting of the commercial design tool PVsyst (v5.6) is compared to the performance measurements of multi crystalline modules in Dietikon, Zurich, during 2011. The total measured horizontal irradiance was 1146 kWh/m2 in 2011. This pyranometer measurement covers 91.5% of the total annual irradiance (determined using IDAWEB data with 100% uptime for Affoltern). The historical irradiance data averaged over decades are about 11.4% (Meteonorm) or 13.6% (PVGIS) lower than the IDAWEB data in 2011. The standard deviation between 2001 and 2011 is about 4.9% with respect to IDAWEB. The measured DC performance ratio (PRDC) is 0.937 with a measurement uncertainty of ±0.031 (k=2). Simulations with manufacturer and optimised parameters showed a deviation to the measurement between 4% and 8%. A second analysis considers shading losses for different shading angles. Calculated losses are about 2% lower than simulated losses with PVsyst using a model of partial shading for a shading angle of 20°. Interesting results could be achieved by determining the loss upon limiting the inverter output power. There is only 4.4% annual loss when the inverter output power is set to a limit of 70 % for the location Dietikon in 2011

Evaluation of PV system performance of five different PV module technologies

Based on the outdoor test PV power plant near Zurich the performance regarding single module and grid connected string of five different cell technologies has been analyzed and compared to indoor measurements. Good correlation between indoor and outdoor behavior has been shown for irradiance around 1000W/m2. For multi c- Si, mono c-Si and a-Si/?c-Si the determined performance values agree within ±2% between indoor and outdoor measurement. Regarding the lowlight performance, evaluation depends on the irradiance reference sensor. While the performance is well determinable regarding self-reference to ISC using c-Si based reference cells shows increasing instability towards lower irradiance due to spectral mismatch. Nevertheless the c-Si reference cell based model has proven to predict energetic output of single module well below measurement uncertainty within ±0.6% for all technologies for the analyzed timeframe and revealed problems in the inverter’s maximum power point tracking

Energy rating based on thermal modelling of five different PV technologies

Minute by minute outdoor measurements of five commercial PV modules including multi c-Si, HIT, a-Si/μc-Si, CIS, CdTe over one year were used to develop a model for predicting the individual module temperatures. The simulated module temperatures over a whole year in minute interval correspond to the outdoor measured values within a RMSE of 1.5°C. Input of the model includes irradiance measurements, which are extracted from the measured short circuit current ISC, the measured wind speed and the ambient temperature. The measured energy weighted module temperature for Zurich was found to be between 33.3°C and 38.0°C for the different module types with a maximum difference of 0.8°C relative to the simulated average module temperature. Due to the high module temperature, the annual average efficiency was reduced relative to the STC values by only 2.6% (HIT) and 5.6% (CIS). Another reduction of the STC efficiency could clearly be attributed to the efficiency at low ISC values which classify low irradiance values. The value is a decrease of the annual energy output of 4.7% and an increase of 1.1%. A further loss analysis was performed to separate spectrum related losses resulting in a relative difference of 2% for the tandem compared to the multi c-Si module

Effect of a four-week isocaloric ketogenic diet on physical performance at very high-altitude ::a pilot study

Background: A ketogenic diet (KD) reduces daily carbohydrates (CHOs) ingestion by replacing most calories with fat. KD is of increasing interest among athletes because it may increase their maximal oxygen uptake (VO2max), the principal performance limitation at high-altitudes (1500–3500 m). We examined the tolerance of a 4-week isocaloric KD (ICKD) under simulated hypoxia and the possibility of evaluating ICKD performance benefits with a maximal graded exercise bike test under hypoxia and collected data on the effect of the diet on performance markers and arterial blood gases. Methods: In a randomised single-blind cross-over model, 6 recreational mountaineers (age 24–44 years) completed a 4-week ICKD followed or preceded by a 4-week usual mixed Western-style diet (UD). Performance parameters (VO2max, lactate threshold [LT], peak power [Ppeak]) and arterial blood gases (PaO2, PaCO2, pH, HCO3−) were measured at baseline under two conditions (normoxia and hypoxia) as well as after a 4-week UD and 4-week ICKD under the hypoxic condition. Results: We analysed data for all 6 participants (BMI 19.9–24.6 kg m−2). Mean VO2max in the normoxic condition was 44.6 ml kg−1 min−1. Hypoxia led to decreased performance in all participants. With the ICKD diet, median values for PaO2 decreased by − 14.5% and VO2max by + 7.3% and Ppeak by + 4.7%. Conclusion: All participants except one could complete the ICKD. VO2max improved with the ICKD under the hypoxia condition. Therefore, an ICKD is an interesting alternative to CHOs dependency for endurance performance at high-altitudes, including high-altitude training and high-altitude races. Nevertheless, decreased PaO2 with ICKD remains a significant limitation in very-high to extreme altitudes (> 3500 m). Trial: registration Clinical trial registration Nr. NCT05603689 (Clinicaltrials.gov). Ethics approval CER-VD, trial Nr. 2020-00427, registered 18.08.2020—prospectively registered

Educational level is related to physical fitness in patients with type 2 diabetes ::a cross-sectional study

Introduction : Low educational level (EL) and low physical fitness are both predictors of increased morbidity and mortality in patients with type 2 diabetes. It is unknown if EL is related to physical fitness. This would have important implication for the treatment approach of patients of low EL. Materials and Methods : In 2011/12, we invited participants of a new nationwide Swiss physical activity program for patients with type 2 diabetes to participate in this study. EL was defined by self-report and categorized as low (mandatory education), middle (professional education) or high (high school/university). Physical fitness was determined using 5 validated measures that assessed aerobic fitness, functional lower limb muscle strength, walking speed, balance and flexibility. Potential confounder variables such as other socio-cultural factors, physical activity level, body composition, diabetes-related parameters and complications/co-morbidities as well as well-being were assessed. Results : All invited 185 participants (mean age 59.6 ±9.8 yrs, 76 women) agreed to be included. Of all patients, 23.1% had a low, 32.7% a middle and 44.2% a high EL; 41.8% were professionally active. The study population had a mean BMI of 32.4±5.2 kg/m2 and an HbA1c of 7.3±1.3%. The mean diabetes duration was 8.8±7.4 years. In the baseline assessment, higher EL was associated with increased aerobic fitness, increased functional lower limb muscle strength, and increased walking speed using linear regression analysis (values for low, middle and high EL, respectively: 91.8 ± 27.9, 116.4 ± 49.7 and 134.9 ± 60.4 watts for aerobic fitness (p = 0.002), 15 ± 4.7, 13.9 ± 2.7, 12.6 ± 2.9 seconds for strength (p = 0.001) and 8.8 ± 1.6, 8.3 ± 1.4, 7.8 ± 1.4 seconds for walking speed (p = 0.004)). These associations were independent of potential confounders. Overall, aerobic fitness was 46%, functional limb muscle strength 16%, and walking speed 11% higher in patients of high compared to those of low EL. EL was not related to balance or flexibility. Discussion : A main strength of the present study is that it addresses a population of importance and a factor (EL) whose understanding can influence future interventions. A second strength is its relatively large sample size of a high-risk population. Third, unlike studies that have shown an association between self-reported fitness and educational level we assessed physical fitness measures by a quantitative and validated test battery using assessors blinded to other data. Another novelty is the extensive evaluation of the role of many relevant confounder variables. Conclusions : In conclusion, we show that in patients with type 2 diabetes EL correlates favorably and independently with important health-related physical fitness measures such as aerobic fitness, walking speed, and lower limb strength. Our findings underline that diabetic patients with low EL should be specifically encouraged to participate in physical activity intervention programs to further reduce social disparities in healthcare. Such programs should be structured and integrate the norms, needs and capacities (financial, time, physical capacities and self-efficacy) of this population, and their effectiveness should be tested in future studies

Relationships of educational level with physical fitness.

<p>Relationships of educational level with physical fitness.</p

Description of the number of persons included and the number of patients used in our analysis.

<p>Description of the number of persons included and the number of patients used in our analysis.</p

Educational Level Is Related to Physical Fitness in Patients with Type 2 Diabetes – A Cross-Sectional Study

<div><p>Introduction</p><p>Low educational level (EL) and low physical fitness are both predictors of increased morbidity and mortality in patients with type 2 diabetes. It is unknown if EL is related to physical fitness. This would have important implication for the treatment approach of patients of low EL.</p><p>Materials and Methods</p><p>In 2011/12, we invited participants of a new nationwide Swiss physical activity program for patients with type 2 diabetes to participate in this study. EL was defined by self-report and categorized as low (mandatory education), middle (professional education) or high (high school/university). Physical fitness was determined using 5 validated measures that assessed aerobic fitness, functional lower limb muscle strength, walking speed, balance and flexibility. Potential confounder variables such as other socio-cultural factors, physical activity level, body composition, diabetes-related parameters and complications/co-morbidities as well as well-being were assessed.</p><p>Results</p><p>All invited 185 participants (mean age 59.6 ±9.8 yrs, 76 women) agreed to be included. Of all patients, 23.1% had a low, 32.7% a middle and 44.2% a high EL; 41.8% were professionally active. The study population had a mean BMI of 32.4±5.2 kg/m² and an HbA1c of 7.3±1.3%. The mean diabetes duration was 8.8±7.4 years. In the baseline assessment, higher EL was associated with increased aerobic fitness, increased functional lower limb muscle strength, and increased walking speed using linear regression analysis (values for low, middle and high EL, respectively: 91.8 ± 27.9, 116.4 ± 49.7 and 134.9 ± 60.4 watts for aerobic fitness (p = 0.002), 15 ± 4.7, 13.9 ± 2.7, 12.6 ± 2.9 seconds for strength (p = 0.001) and 8.8 ± 1.6, 8.3 ± 1.4, 7.8 ± 1.4 seconds for walking speed (p = 0.004)). These associations were independent of potential confounders. Overall, aerobic fitness was 46%, functional limb muscle strength 16%, and walking speed 11% higher in patients of high compared to those of low EL. EL was not related to balance or flexibility.</p><p>Discussion</p><p>A main strength of the present study is that it addresses a population of importance and a factor (EL) whose understanding can influence future interventions. A second strength is its relatively large sample size of a high-risk population. Third, unlike studies that have shown an association between self-reported fitness and educational level we assessed physical fitness measures by a quantitative and validated test battery using assessors blinded to other data. Another novelty is the extensive evaluation of the role of many relevant confounder variables.</p><p>Conclusions</p><p>In conclusion, we show that in patients with type 2 diabetes EL correlates favorably and independently with important health-related physical fitness measures such as aerobic fitness, walking speed, and lower limb strength. Our findings underline that diabetic patients with low EL should be specifically encouraged to participate in physical activity intervention programs to further reduce social disparities in healthcare. Such programs should be structured and integrate the norms, needs and capacities (financial, time, physical capacities and self-efficacy) of this population, and their effectiveness should be tested in future studies.</p><p>Trial Registration</p><p>University of Lausanne clinicaltrials.gov <a href="https://clinicaltrials.gov/ct2/show/NCT01289587" target="_blank">NCT01289587</a></p></div

Presentation of the baseline characteristics of the study population.

<p>Presentation of the baseline characteristics of the study population.</p