A Role for KLF4 in Promoting the Metabolic Shift via TCL1 during Induced Pluripotent Stem Cell Generation

Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is accompanied by morphological, functional, and metabolic alterations before acquisition of full pluripotency. Although the genome-wide effects of the reprogramming factors on gene expression are well documented, precise mechanisms by which gene expression changes evoke phenotypic responses remain to be determined. We used a Sendai virus-based system that permits reprogramming to progress in a strictly KLF4-dependent manner to screen for KLF4 target genes that are critical for the progression of reprogramming. The screening identified Tcl1 as a critical target gene that directs the metabolic shift from oxidative phosphorylation to glycolysis. KLF4-induced TCL1 employs a two-pronged mechanism, whereby TCL1 activates AKT to enhance glycolysis and counteracts PnPase to diminish oxidative phosphorylation. These regulatory mechanisms described here highlight a central role for a reprogramming factor in orchestrating the metabolic shift toward the acquisition of pluripotency during iPSC generation

「学科間プロジェクト」のカリキュラム開発に関する実践的研究/「有馬温泉ゆけむり大学」を事例として

本研究では、「有馬温泉ゆけむり大学」を実践的なデザイン教育の場として研究を行った。すなわち、「学科間プロジェクト」の教育的効果を再考するために、本イベントの「Tシャツ」、「チラシ」デザイン、及び有志学生による「神戸芸工大雑貨屋さん」、「浴衣DEファッションショー」を開催した。その他イベントとして、「有馬温泉クリスマスツリープロジェクト」のワークショップ、「有馬節分会」の「チラシ」デザインを行った。また、武蔵野美術大学・群馬県立女子大学・立命館アジア太平洋大学に出向き、聞き取り調査を行った。研究の結果、「有馬温泉ゆけむり大学」に参加した学生は、他の学科及び学年を超えた交流の機会ができた。また、特に大阪音楽大学・近畿大学・武庫川女子大学の学生たち及び有馬温泉のスタッフとも交流ができたことは、大変貴重な経験となり、様々な教育効果が期待できる。したがって、「学科間プロジェクト」のカリキュラム開発のための基礎資料を得ることができた。In this studies, we studied that the "Arima Onsen Yukemuri University" was for design practical education. That is, in order to rethink the educational effect of "Interdepartmental Project", of this event "T-shirt", "Flyer" design, and by volunteer students "Kobe Design University Zakkaya san", "Yukata DE Fashion show" were held. As other events, workshop of "Arima Onsen Christmas tree project" , "Flyer" design for "Arima Setsubun E".In addition, visited to Musashino Art University, Gunma Prefectural Women\u27s University, Ritsumeikan Asia Pacific University, which were investigated.Results of the studies, students who participated in the "Arima Onsen Yukemuri University" was able opportunity of exchange beyond the school year and other departments. In addition, it becomes a very valuable experience, that the staff of Arima Onsen and students of Osaka College of Music, Kinki University, Mukogawa Women\u27s University also could exchange in particular can be expected to be teaching a variety of effects. Therefore, it was possible to obtain the basic data for curriculum development "Interdepartmental Project"

修士論文要旨 : F. 臨床心理学研究領域

departmental bulletin pape

Distribution of Arbuscular Mycorrhizal Fungi in Upland Field Soil of Japan 1. Relationship Between Spore Density and The Soilenvironmental Factor

To Quantify The Effects of Arbuscular Mycorrhizal Fungi (Am Fungi) On The Growth of Upland Field Crops Cultivated in Japan, We Analyzed Soil Samples From 124 Sites in 18 Japanese Prefectures For Available P Content, Ph and Am Fungal Spore Density. The Am Fungal Spore Density in The 124 Soil Samples Was 1.7 Per G Dw On The Average, and Lower Than 1.0 Per G Dry Soil (Dw) in About Half of The Soil Samples. The Maximum Spore Density Was 20.6 Spores Per G Dw. The Density of Am Fungal Spore Did Not Vary Significantly With The Sampling Site and The Kind of Cultivated Crop in The Sampling Field. The Ph of The Soil With A High Spore Density Ranged From 6 To 8, and in The Soil Samples With A Ph Lower Than 6 and Higher Than 8, TheSpore Density Was Lower Than 5 Spores Per G Dw. Thus, in The Acid Or Alkaline Soil, The Sporogenesis of Am Fungi Is Suppressed. Because Available P Content Was Consistently Low in The Soil Samples With A High Spore Density, P Content Was Considered To Correlate With The Am Fungal Spore Density. Therefore, Crop Cultivation With Limited P Fertilizer Application and Reduced Available P Content May Be Important To increase Am Fungal Spore Density in Upland Field Soil

Recent trends and perspectives in reconstruction and regeneration of intra/extra-oral wounds using tissue-engineered oral mucosa equivalents

Many conditions, including cancer, trauma, and congenital anomalies, can damage the oral mucosa. Multiple cultures of oral mucosal cells have been used for biocompatibility tests and oral biology studies. In recent decades, the clinical translation of tissue-engineered products has progressed significantly in developing tangible therapies and inspiring advancements in medical science. However, the reconstruction of an intraoral mucosa defect remains a significant challenge. Despite the drawbacks of donor-site morbidity and limited tissue supply, the use of autologous oral mucosa remains the gold standard for oral mucosa reconstruction and repair. Tissue engineering offers a promising solution for repairing and reconstructing oral mucosa tissues. Cell- and scaffold-based tissue engineering approaches have been employed to treat various soft tissue defects, suggesting the potential clinical use of tissue-engineered oral mucosa (TEOMs). In this review, we first cover the recent trends in the reconstruction and regeneration of extra-/intra-oral wounds using TEOMs. Next, we describe the current status and challenges of TEOMs. Finally, future strategic approaches and potential technologies to support the advancement of TEOMs for clinical use are discussed

A Role for KLF4 in Promoting the Metabolic Shift via TCL1 during Induced Pluripotent Stem Cell Generation

Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is accompanied by morphological, functional, and metabolic alterations before acquisition of full pluripotency. Although the genome-wide effects of the reprogramming factors on gene expression are well documented, precise mechanisms by which gene expression changes evoke phenotypic responses remain to be determined. We used a Sendai virus-based system that permits reprogramming to progress in a strictly KLF4-dependent manner to screen for KLF4 target genes that are critical for the progression of reprogramming. The screening identified Tcl1 as a critical target gene that directs the metabolic shift from oxidative phosphorylation to glycolysis. KLF4-induced TCL1 employs a two-pronged mechanism, whereby TCL1 activates AKT to enhance glycolysis and counteracts PnPase to diminish oxidative phosphorylation. These regulatory mechanisms described here highlight a central role for a reprogramming factor in orchestrating the metabolic shift toward the acquisition of pluripotency during iPSC generation

R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease

The intestinal microbial ecosystem is actively regulated by Paneth cell-derived antimicrobial peptides such as α-defensins. Various disorders, including graft-versus-host disease (GVHD), disrupt Paneth cell functions, resulting in unfavorably altered intestinal microbiota (dysbiosis), which further accelerates the underlying diseases. Current strategies to restore the gut ecosystem are bacteriotherapy such as fecal microbiota transplantation and probiotics, and no physiological approach has been developed so far. In this study, we demonstrate a novel approach to restore gut microbial ecology by Wnt agonist R-Spondin1 (R-Spo1) or recombinant α-defensin in mice. R-Spo1 stimulates intestinal stem cells to differentiate to Paneth cells and enhances luminal secretion of α-defensins. Administration of R-Spo1 or recombinant α-defensin prevents GVHD-mediated dysbiosis, thus representing a novel and physiological approach at modifying the gut ecosystem to restore intestinal homeostasis and host-microbiota cross talk toward therapeutic benefits