Ectomicorrizas presentes en la plantación trufera “Los Quejigares” (Soria)

En este trabajo se describen las ectomicorrizas presentes en 16 plantas de Quercus ilex L. subsp. ballota (Desf.) Samp. de 22 años, de la finca de 600 ha que gestiona AROTZ-CATESA en el término municipal de Villaciervos (Soria). Además se presentan los porcentajes de micorrización de las ectomicorrizas más interesantes. Este estudio se incluye dentro del proyecto LIFE 99 ENV/E/000356 “Revalorización de bosques productores de trufa: un ejemplo de gestión sostenible”. SUMMARY: This study describes the ectomycorrhiza occurring in 16 twenty-two years old seedlings of Quercus ilex L. subsp. ballota (Desf.) Samp., in the plot of 600 ha that AROTZ-CATESA manages in of Villaciervos (Soria). Mycorrhization percentages of the most interesting ectomycorrhiza are also reported. This study is included in the project LIFE 99 ENV/E/000356 "Truffled Mediterranean forest improvement: an example of sustanaible management”

Quercirhiza quadratum: a revision of the characters and identity of the ad type ectomycorrhiza

The well-known AD type, described first by Giraud in 1988, is considered as a competitor in black truffle (Tuber melanosporum Vittad.) plantations. It has been mainly observed in T. melanosporum and T. magnatum Pico plantations in France and Italy. This ectomycorrhiza has always been observed on roots of oak (Quercus ilex L. and Q. faginea Lam.) and hazelnut (Corylus avellana L.) plantations with “burnt” areas around the trees, even in those that do not produce black truffle sporocarps, so it can create false expectations in young plantations. The AD type has also been described in nurseries, as a competitive ectomycorrhiza on seedlings inoculated with black truffle. In Spain, AD type has been detected in black truffle plantations and natural holm oak stands in Navarra, Soria, Huesca, Zaragoza, Teruel, Castellón and Valencia. In 2005, De Román & De Miguel, suggested that AD type could be a telephoroid type due to its anatomical and morphological characters. In 2006, Baciarelli-Falini et al. using molecular techniques identified this type as an Ascomycotina belonging to Pezizales. The detailed anatomical, morphological and molecular study of the AD type led to a description as Quercirhiza quadratum (Águeda et al. 2008). Based on the anatomical and morphological characters, the AD type belongs to the Ascomycotina. The presence of Woronin bodies on hyphal septa, and the sometimes slightly dissolved septa, are two typical characters of this group. The DNA sequences obtained from the AD types studied showed close similarities with members of Pyronemataceae and Sarcosomataceae (Pezizales). Both taxonomic groups correspond to the same AD type as found by Baciarelli Falini et al., (2006). One of the studied sequences showed a close identity (100% maximum identity, 84% coverage) with Trichophaea woolhopeia (Cooke & W. Phillips) Arnould, although records of this fungal species are scarce in the Iberian Peninsula

Characterization and identification of field ectomycorrhizae of Boletus edulis and Cistus ladanifer

Field ectomycorrhizae sampled under Boletus edulis and Cistus ladanifer have been characterized and described in detail based on standard morphological and anatomical characters. The described ectomycorrhiza has traits typical of Boletales: whitish with three differentiated plectenchymatous layers in the mantle in plan view forming ring-like structures and rhizomorphs with highly differentiated hyphae. The inflated, smooth cystidia-like clavate end cells on the surface of the rhizomorphs and their slightly twisted external hyphae are additional characterizing features. The Hartig net occupies 1 1/2 rows of cortical cells, partly reaching the endodermis. Not all hyphae have clamps. The identification of the fungal symbiont as B. edulis was confirmed by ITS rDNA sequence comparison between mycorrhizas and sporocarps. The singularity of this symbiotic association, as well as its ecological and practical implications, are discussed

Caracterización de ectomicorrizas en encinares productores de trufa negra del noreste de Soria

Black truffle sporocarp production is determined, amongst many other factors, by an optimum mycorrhization degree in the roots of the host-tree. This process is triggered in balance with other fungal species that does not inhibit its sporocarp production. So, there is an ectomycorrhizal fungal community associated with the black truffle sporocarp productive host-trees that runs as any other living beings community, producing a certain fungal biodiversity and establishing connections in dynamic balance that will evolve. The absence of black truffle sporocarps production in some host-trees will be determined by its ectomycorrhiza absence, or because between the fungal community there are one or more species that inhibit this process or displace it. With the aim of study those topics, the ectomycorrhizae present in 23 adult holmoak trees (Quercus ilex subsp. ballota (Desf.) Samp.) of seven Tuber melanosporum Vittad. productive areas in the North-West of Soria (inner Spain) are characterized and quantified. During the spring and the autumn of 1999 and 2000, black truffle productive and non-productive holmoaks were studied following the global method (Verlhac et al., 1990, La truffe guide pratique). Ectomycorrhizal types were characterized following the guidelines of Agerer (1999). Tuber melanosporum, T. aestivum Vittad., T. brumale Vittad., Cenococcum geophylum Fr., Pisolithus arrhizus (Scop.) Rauscher, Cantharellus tubaeformis (Bull.) Fr., Hebeloma cf. sinapizans (Fr.) Sacc., Tomentella galzinii Bourdot, AD type, Cortinarius sp., Hebeloma sp. and Scleroderma sp. and many others Telephorales, Tuberales and Boletales ectomycorrhizal types were found. Tuber melanosporum mycorrhizae are present both in productive and non-productive hosttrees, as it happens for T. aestivum, while T. brumale ectomycorrhizae are only present in nonproductive holmoaks. The rest of identified ectomycorrhizal types are present in productive and non-productive host-trees

Ectomycorrhizal status of a mature productive black truffle plantation

The truffle-plantation «Los Quejigares» was planted in 1971 by AROTZ-CATESA company. It is a 600 ha plot of Quercus ilexmycorrhizated with Tuber melanosporum at 1,250 m a.s.l. on calcareous soil. This plantation is the largest of the world and one of the eldest truffle-plantations of Spain and it is in full production. Knowledge of the mycorrhizal status of a mature black truffle plantation is significant for the improvement of truffle cultivation. Ectomycorrhizae were studied for knowing T. melanosporum persistence and diversity and abundance of other ectomycorrhizal types. Roots of 16 holmoaks were sampled, 12 trees produce truffle sporocarps and four did not. It was found a mean of about 35% of T. melanosporum ectomycorrhizae in the studied trees, being this significantly higher in the productive trees. Also, 105 more different ectomycorrhizal types were found. In spite of the high number of morphotypes found, it seems that they do not replace T. melanosporum, showing that there is a coexistence between species in the fungal community associated to the roots

Síntesis de ectomicorrizas entre Cistus sp. y las especies del complejo Boletus edulis

En los jarales resultantes de la reiteración de incendios forestales en el oeste de Castilla y León (León, Salamanca y Zamora) es habitual la presencia de fructificaciones de Boletus edulis Bull. La recolección de estos hongos micorrícicos comestibles en los jarales dominados exclusivamente por Cistus ladanifer, supone una oportunidad económica alternativa a los usos tradicionales para zonas con bajos ingresos procedentes del monte. El objetivo de éste trabajo es la obtención de ectomicorrizas de las especies del complejo B. edulis con Cistus sp. bajo condiciones controladas, y las descripciones anatómicas detalladas de las mismas. La identificación de los aislados fúngicos de Boletus aereus Bull., B. edulis, B. reticulatus Schaeff. y B. pinophilus Pilát & Dermek fue confirmada por métodos moleculares. Se han obtenido ectomicorrizas de Boletus aereus, B. edulis y B. reticulatus con Cistus albidus L. y C. ladanifer en condiciones de síntesis en cultivo puro, en un substrato de turbavermiculita estéril y solución nutritiva. Las ectomicorrizas formadas se describieron siguiendo los caracteres anatómicos y morfológicos habituales. Las tres ectomicorrizas son muy similares, de color blanco, con ramificación monopodial-pinnada, manto formado por tres capas plectenquimatosas y con rizomorfos de tipo boletoide

Kon-tiki enhances PS2 integrin adhesion and localizes its ligand, Thrombospondin, in the myotendinous junction

Cell-extracellular-matrix adhesion is mediated by cell receptors, mainly integrins and transmembrane proteoglycans, which can functionally interact. How these receptors are regulated and coordinated is largely unknown. We show that the conserved transmembrane Drosophila proteoglycan Kon-tiki (Kon, also known as Perdido) interacts with the αPS2βPS integrin (αPS2 is encoded by inflated and βPS by myospheroid) to mediate muscle-tendon adhesion. kon and inflated double mutant embryos show a synergistic increase in muscle detachment. Furthermore, Kon modulates αPS2βPS signaling at the muscle attachment, since phosphorylated Fak is reduced in kon mutants. This reduction in integrin signaling can be rescued by the expression of a truncated Kon protein containing its transmembrane and extracellular domains, suggesting that these domains are sufficient to mediate this signaling. We show that these domains are sufficient to properly localize the αPS2βPS ligand, Thrombospondin, to the muscle attachment, and to partially rescue Kon-dependent muscle-tendon adhesion.We propose that Kon can engage in a protein complex with αPS2βPS and enhance integrin-mediated signaling and adhesion by recruiting its ligand, which would increase integrin-binding affinity to the extracellular matrix, resulting in the consolidation of the myotendinous junction.B.E. was funded by the Ramón y Cajal program and the Universidad Pablo de Olavide. Research was funded by the Ministerio de Economía y Competitividad (Spanish Ministry of Science and Innovation) (BFU2008-036550, BFU2011-26745). J.J.P.-M. was funded by the Proyecto de Excelencia of the Consejerıa de Economía, Innovación, Ciencia y Empleo, Junta de Andalucıa (PO9-CVI-5058). A.G.E.-Z. and C.B.G.-C. were funded by projects of the Ministerio de Ciencia e Innovación (Spanish Ministry of Science and Innovation) (BFU2008-036550, BFU2011-26745)Peer Reviewe

Ectomicorrizas presentes en la plantación trufera “Los Quejigares” (Soria)

En este trabajo se describen las ectomicorrizas presentes en 16 plantas de Quercus ilex L. subsp. ballota (Desf.) Samp. de 22 años, de la finca de 600 ha que gestiona AROTZ-CATESA en el término municipal de Villaciervos (Soria). Además se presentan los porcentajes de micorrización de las ectomicorrizas más interesantes. Este estudio se incluye dentro del proyecto LIFE 99 ENV/E/000356 “Revalorización de bosques productores de trufa: un ejemplo de gestión sostenible”. SUMMARY: This study describes the ectomycorrhiza occurring in 16 twenty-two years old seedlings of Quercus ilex L. subsp. ballota (Desf.) Samp., in the plot of 600 ha that AROTZ-CATESA manages in of Villaciervos (Soria). Mycorrhization percentages of the most interesting ectomycorrhiza are also reported. This study is included in the project LIFE 99 ENV/E/000356 "Truffled Mediterranean forest improvement: an example of sustanaible management”

Quercirhiza quadratum: a revision of the characters and identity of the ad type ectomycorrhiza

The well-known AD type, described first by Giraud in 1988, is considered as a competitor in black truffle (Tuber melanosporum Vittad.) plantations. It has been mainly observed in T. melanosporum and T. magnatum Pico plantations in France and Italy. This ectomycorrhiza has always been observed on roots of oak (Quercus ilex L. and Q. faginea Lam.) and hazelnut (Corylus avellana L.) plantations with “burnt” areas around the trees, even in those that do not produce black truffle sporocarps, so it can create false expectations in young plantations. The AD type has also been described in nurseries, as a competitive ectomycorrhiza on seedlings inoculated with black truffle. In Spain, AD type has been detected in black truffle plantations and natural holm oak stands in Navarra, Soria, Huesca, Zaragoza, Teruel, Castellón and Valencia. In 2005, De Román & De Miguel, suggested that AD type could be a telephoroid type due to its anatomical and morphological characters. In 2006, Baciarelli-Falini et al. using molecular techniques identified this type as an Ascomycotina belonging to Pezizales. The detailed anatomical, morphological and molecular study of the AD type led to a description as Quercirhiza quadratum (Águeda et al. 2008). Based on the anatomical and morphological characters, the AD type belongs to the Ascomycotina. The presence of Woronin bodies on hyphal septa, and the sometimes slightly dissolved septa, are two typical characters of this group. The DNA sequences obtained from the AD types studied showed close similarities with members of Pyronemataceae and Sarcosomataceae (Pezizales). Both taxonomic groups correspond to the same AD type as found by Baciarelli Falini et al., (2006). One of the studied sequences showed a close identity (100% maximum identity, 84% coverage) with Trichophaea woolhopeia (Cooke & W. Phillips) Arnould, although records of this fungal species are scarce in the Iberian Peninsula

Ectomycorrhizal status of a mature productive black truffle plantation

The truffle-plantation «Los Quejigares» was planted in 1971 by AROTZ-CATESA company. It is a 600 ha plot of Quercus ilexmycorrhizated with Tuber melanosporum at 1,250 m a.s.l. on calcareous soil. This plantation is the largest of the world and one of the eldest truffle-plantations of Spain and it is in full production. Knowledge of the mycorrhizal status of a mature black truffle plantation is significant for the improvement of truffle cultivation. Ectomycorrhizae were studied for knowing T. melanosporum persistence and diversity and abundance of other ectomycorrhizal types. Roots of 16 holmoaks were sampled, 12 trees produce truffle sporocarps and four did not. It was found a mean of about 35% of T. melanosporum ectomycorrhizae in the studied trees, being this significantly higher in the productive trees. Also, 105 more different ectomycorrhizal types were found. In spite of the high number of morphotypes found, it seems that they do not replace T. melanosporum, showing that there is a coexistence between species in the fungal community associated to the roots