From Linkage to GWAS: A Multifaceted Exploration of the Genetic Risk for Alcohol Dependence

Family, twin and adoption studies consistently suggest that genetic factors strongly influence the risk for alcohol dependence (AD). Although the literature supports the role of genetics in AD, identification of specific genes contributing to the etiology of AD has proven difficult. These difficulties are due in part to the complex set of risk factors contributing to the development of AD. These risk factors include comorbidities with other clinical diagnoses and behavioral phenotypes (e.g., major depression), physiological differences that contribute to the differences between people in their level of response to ethanol (e.g., initial sensitivity) and finally the large number of biological pathways targeted by and involved in the processing of ethanol. These complexities have probably contributed to the limited success of linkage and candidate gene association studies in finding genes underlying AD. The powerful and unbiased genome-wide association study (GWAS) offers promise in the study of complex diseases. However, due to the complexities of known risk factors, GWAS data has yet to provide consistent, replicable results. In light of these difficulties, this dissertation has five specific aims which attempt to investigate genetic risk loci for AD and related phenotypes through improved methods for candidate gene selection, analysis of a pooled genome-wide association study, genome-wide analyses of initial sensitivity and maximum alcohol consumption in a twenty-four hour period and finally, creation of a multivariate AD/internalizing phenotype

The Relationship Between Exercise and Depression and Anxiety in College Students

The literature shows an inverse association between exercise and mental disorders. The aim of this study is to further elaborate on this association with regards to exercise and its relationship with anxiety and depression in a college sample. The subject group focused on seniors in the Spit for Science data set which incorporated a total of 821 students. Physical activity was assessed using the International Physical Activity Questionnaire (IPAQ) to estimate the overall metabolic equivalents (MET’s) each student spent in walking, moderate, or vigorous activity levels in the previous week. Sum scores were used to measure depression and anxiety. Overall,the data showed that students 124 students had a walking or low activity level, 255 had a moderate activity level, and 442 had a vigorous or high activity level. There is a significant mean difference in anxiety and depression sum scores between moderate compared to vigorous and moderate compared to low exercise classifications, however no significant mean differences were found between vigorous compared to low exercise groups. Tests showed the correlation between overall MET’s per week compared to anxiety and depression was significant, with an inverse association between the two. This inverse relationship showed that as the overall MET’s increased, the sum score of depression and anxiety both decrease and vice versa. Regression analyses are underway, and covariates are being assessed, for further analyses to determine the relationship between exercise and depression and anxiety. The results of this study can lead to understanding the link between how much exercise is needed to derive a mental benefit as well as where the threshold amount of exercise needed to reverse detrimental effects of inactivity is.https://scholarscompass.vcu.edu/uresposters/1221/thumbnail.jp

Neuroticism as a Moderator of the Relationship Between Family History of Drinking Problems and College Alcohol Use

Literature has long suggested a correlation between family history and alcohol dependence; heredity plays a role in risk for alcohol dependence. To investigate the influence of the personality trait neuroticism as a moderating factor of the relationship between family history of alcohol dependence, and an individual’s alcohol consumption, analyses were conducted in the Fall 2011 Spit for Science cohort. Survey questions examined the binary variable of whether participants reported a family history of drinking problems, as well as the continuous variable measuring the number of alcoholic drinks participants had consumed in the past 30 days. There were a total of 779 responses that included answers to both questions about family history as well as drinking scores in the data and on these, a linear regression and ANOVA was performed. Results show that reports of family history and high levels of neuroticism are both correlated with increased frequency of alcohol use. Analyses continue to examine the moderating effect of neuroticism on the relationship between family history of alcohol use and an individual’s alcohol consumption. These results add more basic information to the literature on alcohol use in college students.https://scholarscompass.vcu.edu/uresposters/1016/thumbnail.jp

The Metabolism of Alcohol: Risk and Protective Factors

Purpose: Abstract for poster submission to VCU Poster Symposium for Undergraduate Research and Creativity Title: The Metabolism of Alcohol: Risk and Protective Factors Background: In 2002, it was reported by the National Institutes of Health that 60.3% of college aged students (18-22) drank alcohol in the past month of being asked, as compared to 51.9% of those not in college. They also found that 20% of college students met the criteria for at least one alcohol use disorder (AUDs)1. Many genes have been linked to an increased risk for AUDs and how individuals with various ethnic backgrounds respond to alcohol. Genes that metabolize alcohol are obvious candidate genes for alcohol-related phenotypes. The purpose of this presentation is to synthesize information about the key genes involved in alcohol metabolism, as documented in the literature. Methods: The information about each gene was found through a literature search using databases including PubMed, Google Scholar, and cited references from relevant papers. For database searches, the names of the genes were used as well as terms such as AUDs, alcohol metabolism, and alcohol and aldehyde dehydrogenase. The focus of the presentation will be relevant to college-aged students, but rely on research done in college and adult populations due to a lack of sufficient college- aged gene-based studies in this area. Results: Alcohol metabolism genes affect how individuals process and respond to alcohol. The main genes that have been studied are alcohol and aldehyde dehydrogenase genes. Alcohol dehydrogenase genes, like ADH1B and ADH1C, first metabolize ethanol (alcohol) into the by-product acetaldehyde and acetaldehyde is then metabolized by aldehyde dehydrogenase genes, like ALDH2, into the waste product acetate2. Many of the adverse effects associated with alcohol consumption are due to the build up of acetaldehyde. Some of these effects include facial blushing, nausea, headaches, and other similar alcohol sensitivity symptoms3,6. Acetaldehyde builds up in an individuals body after alcohol consumption if he or she has a super- active isoenzyme from the ADH1B and/or ADH1C genes and/or a slow ALDH2 enzyme. Individuals with the super-active alcohol dehydrogenase isoenzyme and slow aldehyde dehydrogenase enzyme are less likely to develop AUDs due to the negative effects from excess acetaldehyde in the body4,5,6. However, this also implies that individuals who do not exhibit the described protective phenotype are more likely to endorse increased drinking behaviors and AUDs. Spit for Science: the VCU Student Survey is currently investigating these genes and their association with alcohol consumption and alcohol use disorder symptoms. Due to the diverse nature of the sample, the researchers are able to explore ethnic differences in these alcohol metabolism genes and their effects. Implications: In general, the more public knowledge about these genes and research findings, the more at-risk individuals can get help and diagnosis. A better understanding of aggregate effects of alcohol metabolism genes, as well as more information regarding ethnic differences in the distribution of genetic variants which impact alcohol processing, will assist researchers and health professionals working with those at risk for AUDs.https://scholarscompass.vcu.edu/uresposters/1137/thumbnail.jp

Population Attributable Fraction of Early Age of Onset of Alcohol Use in Alcohol Abuse and Dependence: A 3-Year Follow-Up Study in University Students

Background: we aimed to determine the risk factors and associated population attributable fractions (PAFs) for the age of onset of alcohol use and also to identify protective factors. Methods: we analyzed follow-up data collected between autumn 2011 and spring 2016 (n = 5170) from the first two cohorts (2011, 2012) of the Spit for ScienceTM project. The dependent variables were alcohol abuse and dependence, and the independent variables were age of drinking onset, residence, ethnicity, religiosity, sexual orientation and work status. We determined the odds ratios (OR) using multilevel logistic regression for repeated measures in SPSSv.20. Results: the early onset of alcohol use was associated with an increased risk of alcohol abuse and dependence among females (OR = 14.98; OR = 11.83) and males (OR = 7.41; OR = 6.24). The PAFs for the early onset of alcohol use in alcohol abuse and dependence were respectively 80.9% and 71.7% in females and 71.0% and 63.5% in males. Among females, being white (OR = 1.58; OR = 1.51), living off-campus (OR = 1.73; OR = 2.76) and working full-time (OR = 1.69; OR = 1.78) were also risk factors. Strong religious beliefs were found to protect males from alcohol abuse (OR = 0.58), while same-gender sexual orientation increased the risk among females (OR = 2.09). Conclusion: delaying the age of onset by one year would reduce alcohol abuse among young adultsSpit for Science: The Student Survey was supported by the Virginia Commonwealth University, the NIAAA [P20 AA107828, R37AA011408, K02AA018755, and P50 AA022537] and the National Center for Research Resources and National Institutes of Health Roadmap for Medical Research [UL1RR031990]S

Extending the Apprenticeship of Observation: How Mentee Experience Shape Mentors

Although the importance of mentor teachers in clinical teacher preparation is well established, few researchers explore the social identity development of these individuals. Through our study we contribute to the body of research by exploring mentor teachers\u27 social identity development through the concept of Apprenticeship of Observation - specifically, how they felt their own mentoring experiences influenced their approaches to mentoring. The multi-case study includes findings about mentoring beliefs and practices during the laboratory school component of an Alternate Route to Licensure program. Incorporating semi-structured interviews and video analysis, the findings demonstrate how four mentor teachers\u27 prior experiences as mentees - including Alternate Route to Licensure, traditional teacher preparation programs, and inservice teaching - influenced their interactions with teacher candidates as mentors. Recommendations for practice and implications for future research are provided

Prevalence of Mental Health Disorder Symptoms and Rates of Help-seeking Among University-Enrolled, Black Men

Background. Black men in college represent a subgroup of emerging adults who are at increased risk of developing mental health disorders (MHDs), such as anxiety and depression. Such risk has been attributed to disproportionate experiences with everyday racial discrimination and high levels of psychological distress. Despite being at higher risk, university-enrolled, Black men are not utilizing mental health or health resources at optimal rates. The current evidence base describing prevalence of MHDs and health services utilization among Black men in college is limited. The present study addresses this by examining mental health prevalence among university-enrolled, Black men and their rates of health services utilization. Methods. We analyzed data (N ~ 2500) from a student survey, Spit for Science, a longitudinal, ongoing, research study at a mid-Atlantic, public university. Participants are given surveys in their freshman year and follow-up surveys every spring thereafter. Measures included: mental health disorders (depression and anxiety, as measured by the Symptom Checklist 90) and campus health service utilization (counseling center, health services, wellness center, and recreational sports). We conducted descriptive analyses to determine MHD symptom prevalence and utilization rates; Mann Whitney U tests to compare prevalence rates to White men and Black women; and, Chi-squared tests to compare rates of utilization among groups. Results. During their Freshman year, greater than 60% of students from each ethnic group reported at least one anxiety symptom and greater than 80% reported at least one depressive symptom. By senior year, reporting rates decreased significantly for Black men (49.6%) but remained high for White men (69.1%) and Black women (63%); p \u3c0.000. For depression, results were similar; however, only significant differences between Black men (72.7%) and Black women (87.1%); p\u3c0.000. Black men (20.4%), though reporting high levels of symptoms, still utilized counseling services at lower rates compared to White men (37.76%); p = 0.024. Conclusion. Findings suggest that Black men underutilize available campus health resources despite reporting one or more symptoms associated with anxiety and depression. Further research and prevention efforts are needed to improve help-seeking among this vulnerable population.https://scholarscompass.vcu.edu/gradposters/1077/thumbnail.jp

Filling in the Gaps: Providing Intersectional Support and Intercultural Engagement at a Liberal Arts College

This workshop will share best practices for creating a campus climate that authentically and strategically engages in intersectional competency and support as well as the promotion of intercultural engagement at a small liberal arts college. We will highlight aspects of first generation programming, alliance building between Centers, cultural competency trainings, and inclusive pedagogy initiatives for faculty that contribute to inching closer to social justice and equity. We will conclude the workshop with a conversation about assessing these practices

Patterns of substance use across the first year of college and associated risk factors

Starting college is a major life transition. This study aims to characterize patterns of substance use across a variety of substances across the first year of college and identify associated factors. We used data from the first cohort (N = 2056, 1240 females) of the “Spit for Science” sample, a study of incoming freshmen at a large urban university. Latent transition analysis was applied to alcohol, tobacco, cannabis, and other illicit drug uses measured at the beginning of the fall semester and midway through the spring semester. Covariates across multiple domains – including personality, drinking motivations and expectancy, high school delinquency, peer deviance, stressful events, and symptoms of depression and anxiety – were included to predict the patterns of substance use and transitions between patterns across the first year. At both the fall and spring semesters, we identified three subgroups of participants with patterns of substance use characterized as: (1) use of all four substances; (2) alcohol, tobacco, and cannabis use; and (3) overall low substance use. Patterns of substance use were highly stable across the first year of college: most students maintained their class membership from fall to spring, with just 7% of participants in the initial low substance users transitioning to spring alcohol, tobacco, and cannabis users. Most of the included covariates were predictive of the initial pattern of use, but covariates related to experiences across the first year of college were more predictive of the transition from the low to alcohol, tobacco, and cannabis user groups. Our results suggest that while there is an overall increase in alcohol use across all students, college students largely maintain their patterns of substance use across the first year. Risk factors experienced during the first year may be effective targets for preventing increases in substance use

Prevalence of Nicotine Delivery Systems by Biological Sex in the Spit for Science Study

Nicotine intake usage trends have changed over recent decades given the wide variety of nicotine delivery systems including cigarettes, vaping, hookah, and snubs/chewables. These trends also vary by demographic factors, such as race/ethnicity, sex, and socioeconomic status (SES). For example, studies in rat populations, as well as humans, have found that females tend to be more dependent on nicotine products and have a more difficult time quitting than male rats and humans (Pogun et al., 2017). Also, race/ethnicity may impact the frequency of nicotine usage in different populations; in that non-white Hispanics were more susceptible to smoking through adolescence with a peak at ages 12 and 16; whereas non-Hispanic Asian Americans were less susceptible to smoking at ages 11 and 15 (El-Toukhy et al., 2016). Certain nicotine delivery methods may be more available or perhaps more socially accepted by certain groups of people. While lower SES is associated with more prevalent cigarette advertisements and usage, individuals with a higher SES were associated with an increased prevalence of e-cigarette advertisements, leading to an increased frequency of e-cigarette usage among adolescents (Simon et al., 2018). This project aims to document rates of nicotine use across different nicotine delivery systems in college students by demographic factors. We use the Spit for Science (S4S) database to investigate prevalence rates and study if they differ by sex, race/ethnicity, or SES. It is hypothesized that higher SES individuals will have an increased frequency of use with nicotine products that are non-cigarette based, non-white Hispanics will have greater frequency with nicotine usage, and females within the study sample will display a higher dependency on nicotine products than males. Preliminary analyses reveal that there are more female participants than males throughout the S4S cohorts collected between 2020 and 2022. Across cohorts, prevalence of all nicotine delivery systems differs in female and male participants across all products. Larger differences in prevalence between females and males are observed for cigarettes, smokeless tobacco, and cigars than for products that have been introduced more recently, such as hookah, vaping, and heat-not-burn products. Further analyses will focus on patterns of use in relation to race/ethnicity and SES. Understanding nicotine usage trends within our sample could pave the way for additional research (i.e., genetic studies) and allow for the development of prevention/intervention models tailored to our sample populations.https://scholarscompass.vcu.edu/uresposters/1436/thumbnail.jp