Plan de emprendimiento para la creación de empresas en la IV región del país

El presente plan de negocio consiste en una propuesta de emprendimiento de la pequeña microempresa CARSOL, dedicada a la producción y comercialización de adornos a base de cartón, cuyo propósito es abastecer el mercado de objetos utilitarios, que a la vez sirvan como adornos para mantener ordenados pequeños espacios en oficinas y hogares. Esta pequeña empresa estará ubicada en el municipio de Nandaime del departamento de Granada. Para el diseño de la propuesta de inversión se realizaron los estudios pertinentes para demostrar su factibilidad comercial, técnica, organizativa y financiera. En la parte comercial se realizó un sondeo de mercado que permitió conocer el potencial de éxito del nuevo negocio. Además, se definieron las estrategias comerciales que permitirán a CARSOL posicionarse en el mercado de artesanías. En el plan de producción se identificaron las necesidades de instrumentos de trabajo necesarios para impulsar el proceso productivo, se cuantificaron las necesidades y fuentes de materias primas, así como el proceso productivo que se deben realizar para la producción de los diferentes bienes que generará CARSOL. En el aspecto organizativo se identificaron los requisitos legales y administrativos que debe cumplir el negocio para su operación además de las necesidades y costos de mano de obra. Por último, en el plan financiero, se estructuraron todos los estados financieros en los que se plasman los datos generados por las ventas y los costos de operación durante los cinco años de vida útil del negocio, así como los indicadores financieros que demuestran la factibilidad de la puesta en marcha de CARSO

Genetic structure in the paternal lineages of South East Spain revealed by the analysis of 17 Y-STRs

Supplementary information accompanies this paper at https://doi.org/10.1038/s41598-019-41580-9.The content of this article is part of the Ph.D. thesis of María Saiz which was conducted at the University of Granada under the doctoral programme “Biomedicine”. The authors thank all of the participants who donated buccal swabs and all those who helped in the sample collection—namely, María Luisa Aceituno Villalva, Leticia Olga Rubio Lamia, and Verónica Delgado López. In addition, the authors want to thank Xiomara Gálvez for the technical assistance in the laboratory.The genetic data of 17 Y chromosome short tandem repeats in 146 unrelated donor residents in the provinces of Granada, Málaga, and Almería (GMA) were analyzed to determine the genetic legacy of the male inhabitants of the former Kingdom of Granada. A total of 139 unique haplotypes were identified. Observed allele frequencies and haplogroup frequencies were also analyzed. By AMOVA and STRUCTURE analysis, the populations of the 3 provinces could be treated genetically as a single population. The most frequent haplogroup was R1b1b2 (58.22%). By network analysis of all individuals, we observed a distribution according to haplogroup assignment. To improve the characterization of GMA population, it was compared with those of North Africa, the Iberian Peninsula, and southern Europe. In our analysis of allele frequencies and genetic distances, the GMA population lay within the Spanish population group. Further, in the STRUCTURE analysis, there was no African component in the GMA population, confirming that, based on our genetic markers, the GMA population does not reflect any male genetic influence of the North African people. The presence of African haplogroups in the GMA population is irrelevant when their frequency is compared with those in other European populations

Interaction of 8 He with 208Pb at near-barrier energies: 4 He and 6 He production

Spanish Ministry of Economy and Competitiveness-FPA-2010-22131-CO2-01 (FINURA) y FPA2013-47327-C2-1-RMinistry of Science and Higher Education of Poland-N202 033637National Science Centre of Poland-2013/08/M/ST2/00257 (LEA-COPIGAL) y 2014/14/M/ST2/00738 (COPIN-INFN Collaboration)European Science Foundation-EUI2009-04163432 (EUROGENESIS

Identification of MiRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer

MiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis. Here, we report the role of several miRNAs’ expression patterns, such as miR-miR-93-5p, miR-23c, miR-210-3p, miR-221-3p, miR-592, miR-141, miR-375, and miR-130b, with relevance in processes like cell proliferation and MET. Using Trizol® extraction protocol and TaqMan™ specific probes for amplification, we performed miRNAs’ analysis of 159 PC fresh tissues and 60 plasmas from peripheral blood samples. We had clinical data from all samples including PSA, Gleason, TNM, and D’Amico risk. Moreover, a bioinformatic analysis in TCGA (The Cancer Genome Atlas) was included to analyze the effect of the most relevant miRNAs according to aggressiveness in an extensive cohort (n = 531). We found that miR-210-3p, miR-23c, miR-592, and miR-93-5p are the most suitable biomarkers for PC aggressiveness and diagnosis, respectively. In fact, according with our results, miR93-5p seems the most promising non-invasive biomarker for PC. To sum up, miR-210-3p, miR-23c, miR-592, and miR93-5p miRNAs are suggested to be potential biomarkers for PC risk stratification that could be included in non-invasive strategies such as liquid biopsy in precision medicine for PC management.Regional Ministry of Health and Families of the Andalusian Government (ref: PI-0319-2018)FIBAO (Andalusian Public Foundation for Biomedical Research in Eastern Andalusia, “Alejandro Otero”

Voltammetric study of the adsorbed thermophilic plastocyanin from Phormidium laminosum up to 90 °c

Redox thermodynamics and kinetics of plastocyanin from Phormidium laminosum, and of azurin from Pseudonomas aeruginosa, have been investigated as a function of temperature by protein film voltammetry. To this purpose, both proteins have been physisorbed on a pyrolytic graphite edge electrode. A pronounced negative shift of the plastocyanin standard potential, compared to a slight shift in the case of azurin, has been found upon increasing the temperature. Hence, significant conformational and/or solvation changes accompany the redox conversion of plastocyanin. Lower electron transfer rate constants (by c.a. one order of magnitude) and higher activation enthalpies have been found for plastocyanin as compared to azurin. The voltammetric response of azurin vanishes irreversibly at temperatures close to 60 °C, whereas the redox properties of plastocyanin remain unaltered, except for some loss of electroactive protein, after heating the electrode at temperatures as high as 90 °C.Ministerio de Economía y Competitividad CTQ 2008-00371, BFU2009-07190Junta de Andalucía P07-FQM-02492, P06-CVI-0171

Combined Genome, Transcriptome and Metabolome Analysis in the Diagnosis of Childhood Cerebellar Ataxia

Supplementary Materials: Supplementary Materials can be found at https://www.mdpi.com/1422 -0067/22/6/2990/s1.The project was approved by the Biomedical Research Ethics Committee of the Andalusian Public Health System in Granada, Spain on Oct. 29, 2015, (project identification code AP163052016). Data recording, sample collection and all in vitro experiments were conducted in accordance with ethical guidelines following the Nuremberg Code, Belmont Report, and the Declaration of Helsinki.Acknowledgments: We are grateful for the support and cooperation of the subjects, as well as their families.Ataxia in children is a common clinical sign of numerous neurological disorders consisting of impaired coordination of voluntary muscle movement. Its most common form, cerebellar ataxia, describes a heterogeneous array of neurologic conditions with uncountable causes broadly divided as acquired or genetic. Numerous genetic disorders are associated with chronic progressive ataxia, which complicates clinical management, particularly on the diagnostic stage. Advances in omics technologies enable improvements in clinical practice and research, so we proposed a multi-omics approach to aid in the genetic diagnosis and molecular elucidation of an undiagnosed infantile condition of chronic progressive cerebellar ataxia. Using whole-exome sequencing, RNA-seq, and untargeted metabolomics, we identified three clinically relevant mutations (rs141471029, rs191582628 and rs398124292) and an altered metabolic profile in our patient. Two POLR1C diagnostic variants already classified as pathogenic were found, and a diagnosis of hypomyelinating leukodystrophy was achieved. A mutation on the MMACHC gene, known to be associated with methylmalonic aciduria and homocystinuria cblC type, was also found. Additionally, preliminary metabolome analysis revealed alterations in our patient’s amino acid, fatty acid and carbohydrate metabolism. Our findings provided a definitive genetic diagnosis reinforcing the association between POLR1C mutations and hypomyelinating leukodystrophy and highlighted the relevance of multi-omics approaches to the disease.Fundación Mutua Madrileña, Spain XIII Call on Research Grants 2016, reference number AP16305201

Simultaneous analysis of the elastic scattering and breakup channel for the reaction 11 Li + 208 Pb at energies near the Coulomb barrier

We present a detailed analysis of the elastic scattering and breakup channel for the reaction of Li11 on Pb208 at incident laboratory energies of 24.3 and 29.8 MeV, measured at the radioactive ion beam facility of TRIUMF, in Vancouver, Canada. A large yield of Li9 fragments was detected by four charged particle telescopes in a wide angular range. The experimental angular and energy distributions of these Li9 fragments have been compared to coupled-reaction-channel and continuum-discretized coupled-channel calculations. The large production of Li9 fragments at small angles can be explained by considering a direct breakup mechanism, while at medium-large angles a competition between direct breakup and neutron transfer to the continuum of the Pb208 target was observed.Proyecto Nacional (España) PA2009-08848 PA2009-07387 PA2010-22131-C02-01 FPA2009-07653 FPA2012-32443Programa Consolider-Ingenio 2010 (España) CSD2007-0004

Cuentos felinos 3

El cuento en la región Caribe de Colombia es un género consolidado, siempre en alza. Es una tipología narrativa que les viene a la medida a autores que tradicionalmente le han echado mano para indagar con plenos poderes en los más intrincados caminos de su historia. Aunque pertenecientes a distintas generaciones, dueños de particulares obsesiones temáticas, a todos los hermanos una poética que encuentra en el cuento un vehículo de reflexión sumamente dúctil. Esta selección de Cuentos felinos 3, ampliada con el mayor de los cuidados, reitera en los autores una concepción de la literatura en la que nunca faltan la complicidad, el humor y el juego

Scattering of the Halo Nucleus 11Be on 197Au at Energies around the Coulomb Barrier

Angular distributions of the elastic, inelastic, and breakup cross sections of the halo nucleus 11Be on197 Au were measured at energies below (Elab =31.9 MeV) and around (39.6 MeV) the Coulomb barrier. These three channels were unambiguously separated for the first time for reactions of 11Be on a high-Z target at low energies. The experiment was performed at TRIUMF (Vancouver, Canada). The differential cross sections were compared with three different calculations: semiclassical, inert-core continuum-coupled-channels and continuum-coupled-channels ones with including core deformation. These results show conclusively that the elastic and inelastic differential cross sections can only be accounted for if core-excited admixtures are taken into account. The cross sections for these channels strongly depend on the β (E1) distribution in 11Be, and the reaction mechanism is sensitive to the entanglement of core and halo degrees of freedom in 11Be.Ministerio de Economía y Competitividad FPA2015-64969-P, FIS2014-53448-C2-1-P, FPA2013-47327-c02-01-R, FPA2012-32443, FIS2013-41994-PCentro Nacional de Física de Partículas, Astropartículas y Nuclear CSD2007- 00042European Collaborative Research EUI-2009-04162European Unions 654002Nuclear Astrophysics Virtual Institute VH-VI-417European Commission, Seventh Framework Program 60037