EVALUATING THE MECHANISMS OF UNCONJUGATED BILIRUBIN TOXICITY THROUGH ANALYSIS OF THE DISULFIDE PROTEOME

Unconjugated bilirubin (UCB), the end product of heme catabolism, is an important physiological antioxidant at nMolar concentrations, but becomes a pro-oxidant at pMolar concentrations by causing the release of reactive oxygen species (ROS) from mitochondria. We employed diagonal redox two-dimensional polyacrylamide gel electrophoresis (R2D-PAGE), to separate and identify specific proteins by mass spectrometry and determined the proteomic signature of the disulfide proteome in different subcellular fractions of Hepa lclc7 cells treated with pro-oxidant (50 pM) or antioxidant (70 nM) concentrations of UCB in Hepa lclc7 cells. Our results demonstrate that treating cells with pro-oxidant or antioxidant concentrations of UCB had a significant and different change on disulfide bonded proteins in the cytosol, with notable effects on peroxiredins (Prx). Pro-oxidant concentrations of UCB led to the irreversible oxidation of Prxl with no effect on Prx2, whereas antioxidant concentrations led to an enhanced reduction in Prx2 with no effect on Prxl

Hubungan Tingkat Stres Remaja Dengan Kemampuan Beradaptasi Di Asrama Pondok Pesantren Sabrun Jamil

Severe stress can make the adaptability of adolescents adaptive or maladaptive and threaten a person's ability to deal with their environment. The aim of this study was to determine the relationship between adolescent stress levels and adaptability. This is a quantitative observational analytical study with a cross sectional design. The sample size was 69 teenagers who were selected using proportional stratified random sampling. Data were collected using the Depression Anxiety Stress Scale (DASS 42) questionnaire for stress levels and the Individual Adaptability (I-ADAPT) questionnaire for adaptability. The data were analyzed using the chi-square test statistical test. The results showed that adolescents who experienced severe stress with maladaptive adaptability of 30 (54.5%). The results of the analysis show that there is a significant relationship between adolescent stress levels and adaptability with p value = 0.000 = 0.05. there is a relationship between stress levels and adaptability in adolescents. counseling guidance is needed for students who experience stress and the ability to adapt

RELATIONSHIP BETWEEN BODY MASS INDEX (BMI) AND AGE OF MENARCHE IN ADOLESCENT GIRLS AT MTs NEGERI 3 GORONTALO REGENCY

Menarche adalah haid yang pertama kali dialami oleh setiap remaja putri yang sudah memasuk masa pubertas. Setiap remaja putri memiliki usia menarche yang berbeda-beda, hal ini bisa dipengaruhi oleh faktor keturunan, faktor gizi, dan kesehatan umum lainnya. Kebaruan penelitian ini karena meneliti tentang hubungan Indeks Massa Tubuh (IMT) dengan usia menarche pada remaja putri di MTs Negeri 3 Kabupaten Gorontalo. Penelitian ini bertujuan untuk mengetahui hubungan indeks massa tubuh dengan usia menarche pada remaja putri di MTs Negeri 3 Kabupaten Gorontalo. Penelitian ini merupakan penelitian kuantitatif dengan pendekatan observasional menggunakan desain Cross Sectional, pengumpulan data menggunakan lembar observasi, analisis data menggunakan uji non parametrik dengan analisis spearman rank. Hasil penelitian yaitu siswi yang memiliki usia menarche normal terdapat 70 responden (56,5%) dan yang memiliki usai menarche lambat terdapat 54 reponden (43,5%), dengan rata-rata indeks massa tubuh yaitu 19,65. Hubungan indeks massa tubuh dan usia menarche diperoleh dengan nilai koofisien korelasi sebesar -0,622 dengan nilai p-value 0,000 ≤ α = 0,05. Simpulan terdapat hubungan antara indeks massa tubuh dengan usia menarche pada remaja putri di MTs Negeri 3 Kabupaten Gorontalo. Kata Kunci : IMT; Usia Menarche; Remaja Putri.  Abstract            Menarche is the first menstruation experienced by every teenage girl who has begun puberty. Every young woman has a different age of menarche; this can be influenced by heredity, nutritional factors, anf other general health. The novelty of this study is because it examines the relationship between Body Mass Index (BMI) and menarche age in young women in MTs Negeri 3, Gorontalo Regency. This study answered the question of whether  there is a relationship between body mass index (BMI) and age of menarche in adolescent girls at MTs Negeri 3 Gorontalo Regency. This research is quantitative research with an observational approach using a cross sectional design, data collection using observation sheets, and data analysis using a non-parametric test with spearman rank analysis. The results of the study show that students who had normal menarche age were 70 respondents (56.5%), and those who had late menarche were 54 respondents (43.5%), followed by an average body mass index of 19.65. The relationship between body mass index and age of menarche was obtained with a correlation coefficient of -0.622 with a p-value of 0.000—0.05. The conclusion is that there is a relationship between body mass index and age of menarche in adolescent girls at MTs negeri 3 Gorontalo Regency. Keywords: BMI; Age of Menarche; Adolescent Girl

Co-management mangrove ecosystem in The Langge Village, Anggrek District, North Gorontalo Regency

This research aimed to 1) determine the existing condition of Langge Village's mangroves and their utilization, 2) analyze stakeholders, the influence and mapping of co-management, and 3) analyze the form of co-management, roles, and responsibilities of stakeholders towards mangrove management in Langge Village. This research was conducted in August-November 2021. Mangrove vegetation data was collected using the line transect method; while the stakeholder data collection is done by open interviews. The data obtained from mangrove vegetation are frequency and density; while the stakeholders are in the form of interests, influence, implementation of co-management, and the roles and responsibilities of stakeholders. Based on this study, 16 species of true mangroves were found at 3 observation stations from Avicennia, Bruguiera, Ceriops, Rhizophora, Sonneratia, and Xylocarpus groups. Mangrove management in Langge Village is at the Advisory Committee stage or the advocacy stage.Keywords: Co-Management, stakeholders, management, mangrov

Pelaksanaan Sistem Informasi Manajemen (SIM) Di Puskesmas Sipatana Menggunakan Metode Human Organization Technology Fit (Hot-Fit)

Sistem Informasi Manajemen Puskesmas adalah Tatanan manusia/peralatan yang menyediakan informasi untuk membantu proses manajemen puskesmas mencapai sasaran kegiatan. Pelaksanaan SIMPUS di Puskesmas Sipatana belum maksimal. Masih ada bagian pemegang program yang belum menggunakan SIMPUS, hanya melakukan pencatatan dan pelaporan secara manual dan kadang juga petugas masih sering terlambat dalam penginputan data. Jenis penelitian adalah kuantitatif. Dengan menggunakan strategi total sampling, jumlah sampel dalam penelitian ini sama dengan jumlah populasi yaitu 25 responden yang menggunakan SIMPUS. Instrumen penelitian menggunakan kuisioner. Teknik analisis data menggunakan Metode Human Organization Technology Fit. Hasil penelitian ini menunjukan bahwa dari hasi hitung yang dilakukan menggunakan metode HOT-Fit di peroleh aspek human bernilai (3,10), aspek organization bernilai (2,99), aspek technology bernilai (2,97) dan aspek benefit bernilai (3). Pelaksanaan SIMPUS di Puskesmas Sipatana sudah baik berdasarkan metode HOT-Fit. Namun masih ada beberapa kendala yang ditemukan seperti kurangnya pemahaman mengenai sistem yang digunakan dan kendala jaringan

A Competitive Study Using Electrospinning and Phase Inversion to Prepare Polymeric Membranes for Oil Removal

Polyacrylonitrile (PAN) is a popular polymer that can be made into membranes using various techniques, such as electrospinning and phase inversion. Electrospinning is a novel technique that produces nonwoven nanofiber-based membranes with highly tunable properties. In this research, electrospun PAN nanofiber membranes with various concentrations (10, 12, and 14% PAN/dimethylformamide (DMF)) were prepared and compared to PAN cast membranes prepared by the phase inversion technique. All of the prepared membranes were tested for oil removal in a cross-flow filtration system. A comparison between these membranes’ surface morphology, topography, wettability, and porosity was presented and analyzed. The results showed that increasing the concentration of the PAN precursor solution increases surface roughness, hydrophilicity, and porosity and, consequently, enhances the membrane performance. However, the PAN cast membranes showed a lower water flux when the precursor solution concentration increased. In general, the electrospun PAN membranes performed better in terms of water flux and oil rejection than the cast PAN membranes. The electrospun 14% PAN/DMF membrane gave a water flux of 250 LMH and a rejection of 97% compared to the cast 14% PAN/DMF membrane, which showed a water flux of 117 LMH and 94% oil rejection. This is mainly because the nanofibrous membrane showed higher porosity, higher hydrophilicity, and higher surface roughness compared to the cast PAN membranes at the same polymer concentration. The porosity of the electrospun PAN membrane was 96%, while it was 58% for the cast 14% PAN/DMF membrane.</p

Performance of Kampung Unggul Balitnak (KUB) Chickens with the Additions of Soya Flour in the Diet

Ayam Kampung Unggul Balitnak (KUB) is the result of selecting native Indonesian kampong chicken strains for six generations that have advantages in terms of maintenance due to the level of feed consumption that is less. The aim of this research was to the effect of soybean flour on the performances of Balitnak Superior Kampong Chicken (KUB). This study used a completely Randomised Design consisting of 5 treatments and 4 replicates. The treatments consisted of T1: basal + 0% soya flour; T2: basal + 2,5% soya flour; T3: basal + 5% soy flour; T4: basal + 7.5% soya flour and T5: basal + 10% soya flour. The results showed that the use of soya flour in the ration of KUB chickens gave an increase in ration consumption, weight gain and could increase the efficiency of ration use. The best concentration of soya flour in the KUB chicken ration was 10%

IDENTIFIKASI RISIKO KESELAMATAN DAN KESEHATAN KERJA PADA PENGOPERASIAN INCINERATOR

Keselamatan dan kesehatan kerja (K3) adalah usaha untuk sedapat mungkin menjamin keselamatan dan kesehatan pada pekerja sehingga pekerja dapat merasa aman dan nyaman bekerja ditempatnya. Tujuan penelitian untuk mengidentifikasi risiko keselamatan dan kesehatan kerja pada pengoperasian incinerator di Rumah Sakit Umum Daerah (RSUD) Prof. Dr. H. Aloei Saboe Kota Gorontalo. Desain penelitian ini merupakan desain deskriptif dengan pendekatan mixed method. Informan penelitian terdiri dari 1 informan kunci, 1 informan utama dan 1 informan pendukung. Pengumpulan data melalui observasi, wawancara terstruktur dan dokumentasi. Hasil penelitian bahwa pada setiap pekerjaan di area pengoperasian incinerator berpotensi bahaya mekanis, listrik, biologi, kimia, dan ergonomi. Hasil penilaian risiko didapatkan 1 risiko tinggi sehingga memerlukan tindakan lanjutan dan 60 risiko rendah sehingga risiko dapat diterima. Faktor penyebab risiko tertinggi didapatkan 31 akar penyebab permasalahan seperti pekerja tidak mengikuti SOP dan pekerja kurang pengetahuan. Rekomendasi pengendalian terhadap faktor penyebab risiko tertinggi dan didapatkan 10 rekomendasi pengendalian administratif. Simpulan bahwa terdapat 1 risiko tertinggi yaitu cedera ringan/berat akibat tidak menggunakan APD lengkap