Transformation of Cm-ADH gene to melon genotype

Alcohol dehydrogenases (ADH) participate in the biosynthetic pathway of aroma volatiles in fruit by interconverting aldehydes to alcohols and providing substrates for the formation of esters. Two highly divergent ADH genes of cantaloupe Charentais melon (Cucumis melo var. cantalupensis 'Védrantais') have been isolated. This research is aimed at identifying the role of the Cm-ADH genes (Cm-ADH1 and 2) in the synthesis of aroma volatiles via the RNAi method. Different regeneration media were investigated to improve transformation efficiency. The best regeneration result was obtained from MS medium supplemented with 2.2 µM BAP and 0.54 µM NAA. Regeneration protocol has been applied to obtain transformed plants, using Agrobacterium tumefaciens which has a vector RNAi pFGC5941-(11406 bp) carrying genes of resistance to kanamycine and Bar. Transformed plants were characterized by PCR analysis.Grant: Thanks to Cukurova University Academic Research and Projects Units (Project No: ZF2009YL36) for financial support

Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata

DOI: 10.1016/S0168-9452(03)00069-4International audienc

Zooplankton abundance and vertical fluxes of sedimentary matter and chemical elements in the White Sea from 15 to 24 June, 2000

A multidisciplinary oceanographic survey of the White Sea was carried out in the Gorlo Straight, Basin, and Kandalaksha Bay regions including estuaries of Niva, Kolvitza and Knyazhaya rivers. Hydrophysical study in the northern part of the Basin revealed long-lived step-like structures and inversions in vertical profiles of temperature and salinity, which formed due to tidal mixing of saline and cold Barents Sea waters and warmer White Sea waters in the Gorlo Straight. Biological studies revealed the main features of spatial distribution, as well as qualitative and quantitative composition of phyto- and zooplankton in all studied areas; tolerance of main zooplankton species to fresh water influence in estuaries was shown. Study of suspended matter in estuaries clearly demonstrated physicochemical transformations of material supplied by the rivers. Data on vertical particle flux in the deep part of the Kandalaksha Bay showed difference between the upper and near-bottom layers, which could result from sinking of spring phytoplankton bloom products and supply of terrigenic suspended matter from the nepheloid layer formed by tidal currents