Lay perceptions of risk factors for Rift Valley fever in a pastoral community in northeastern Kenya

Authors thank the community in Ijara for participating in the study and Millicent Liani for assisting with data collection and providing access to relevant literature. We acknowledge the Food Safety and Zoonoses Research Program at the International Livestock and Research Institute in Nairobi (funded by the CGIAR research program on Agriculture for Nutrition and Health (A4NH) led by the International Food Policy Research Institute. Part of this work was supported by the project ‘Dynamic Drivers of Disease in Africa: Ecosystems, livestock/wildlife, health and wellbeing: REF:NE/J001422/1” funded by the Ecosystem Services for Poverty Alleviation Programme (ESPA). The ESPA program is funded by the Department for International Development (DFID), the Economic and Social Research Council (ESRC) and the Natural Environment Research Council (NERC).Background: Human behavioral factors have been found to be central in the transmission of Rift Valley fever. Consumption of contaminated meat and milk in particular have been identified as one of the key risk factors for the transmission of Rift Valley fever in humans. In pastoral communities, livestock is the main source of livelihood from which many benefits such as food as well as economic and cultural services are derived. Zoonotic diseases therefore have a great impact on pastoral communities livelihoods. However, lay perceptions regarding the transmission of these diseases including Rift Valley fever hampers their effective control. This study investigated the lay perceptions of risks for Rift Valley fever transmission in a pastoral community in northeastern Kenya. Methods: A qualitative study was carried out in Ijara district, Kenya which was one of the hotspots of Rift Valley during the 2006/2007 outbreak. Data were collected using focus group discussions and narratives guided by checklists. Eight focus group discussions consisting of 83 participants and six narratives were conducted. Data was transcribed, coded and analysed according to Emergent themes. Results: The participants reported that they had experienced Rift Valley fever in their livestock especially sheep and in humans both in 1997/1998 and 2006/2007. However, they believed that infections in humans occurred as a result of mosquito bites and had little to do with their consumption of meat, milk and blood from infected livestock. The participants in this study indicated that they had heard of the risks of acquiring the disease through consumption of livestock products but their experiences did not tally with the information they had received hence to them, Rift Valley fever was not transmissible through their dietary practices. Conclusions: Though the communities in this region were aware of Rift Valley fever, they did not have elaborate information regarding the disease transmission dynamics to humans. To avoid misconception about transmission of the disease, intervention strategies, require to be accompanied by comprehensive explanations of the dynamics of its transmission. It is necessary to develop appropriate interventions that take into consideration, lay perceptions of risk factors for the disease and communities’ livelihood strategies.Publisher PDFPeer reviewe

Antimicrobial Susceptibility Profiles of Non-Typhoidal Salmonella Isolates Obtained from Food Animals in Selected Sites in Kenya

The organisms Salmonella are known to be facultative intracellular pathogens that can survive in the host macrophages. The Strains of Non-Typhoidal Salmonella (NTS) are a major cause of serious bloodstream infections with a case fatality of 20-25%. In most cases, the clinical presentations include: gastroenteritis, bacteremia, focal infection and enteric fever. In other continents of the world, NTS are associated with other clinical presentations but not majorly diarrhea. This study aims to determine the antimicrobial susceptibility profiles and resistance genes transferability of Non Typhoidal Salmonella isolates obtained from food animals (Cattle, Chicken and Pigs) and the environment in selected sites in Kenya at the Center for Microbiology Research (CMR) Institute. During this study, some of the laboratory methods that were carried out included antimicrobial susceptibility testing using the disk diffusion technique for all commonly used antimicrobials and Conjugation Assay to determine Transferable Resistance determinants. This study yielded relevant findings concerning the increasing rate of Salmonellosis in this country. The study first of all agrees with other studies that there is a high rate of NTS in food animals and this has a great connection with the increased population of these organisms in the environment. The high rate in continuous usage of antibiotics for treatment of infections in food animals and also to increase products production especially in chicken has led to the rise of resistance cases to commonly used antibiotics. Conjugation studies carried out revealed that resistant genes transferability from one bacterial species to the other is common.  A total of 289 isolates used in this study, A total of 289 NTS samples were analyzed. Among the 289 samples, 134 (46%; 95% C.I: 40.3%-51.8%) were chicken isolates, 45 (16%; 95% C.I: 11.2%-20.3%) were cow isolates, 27 (9%; 95% C.I: 5.7%-12.3%) were pig isolates, 3 (1%; 95% C.I: -0.15%-2.15%) were isolates from goats and environmental isolates were 80 (28%; 95% C.I: 22.8%-33.2. The highest levels of resistance were against Ampicillin 42.7% had the highest resistance level followed by Sulfamethoxazole/Trimethoprim 38.2% and then Chloramphenicol 22.2%. The proportion of isolates resistant against Gentamicin was 9.4%. Environmental isolates showed a higher resistance profile against Ceftazidime 2.4%, Ceftriaxone 2.4%, Sulfamethoxazole/Trimethoprim 40.2% and Chloramphenicol 25.6% compared to food animal isolates. %). It is of importance that there be strict control on the use of antibiotics in animal production to prevent the emergence of antibiotic resistance in Non-Typhoidal Salmonella. Keywords: Invasive Non-Typhoidal Salmonella, antimicrobial resistance, conjugation, resistance genes transferability, MDRs DOI: 10.7176/JBAH/9-2-0

Survey of urban mosquitoes species (Diptera: Culicidae) with focus on waste water channels as larval habitats in Nairobi industrial area, Kenya

Background: A cross sectional study to establish the levels of heavy metals and other potentially harmful elements (PHEs)present in samples obtained from selected open waste water channels in Nairobi industrial area (Kenya) was carried out. The waste water channels selected were those near the factories or those directly discharging from factories. The samples collected included mosquitoes (larvae and adults), waste water, green algae, and soil. Unmaintained open waste water channels are among the man made features that enhance the breeding of urban mosquitoes because they tend to have overgrown vegetation and trapped solid wastes which slow or inhibit the waste water flow. Different mosquito species have previously transmitted arboviruses including those responsible for dangerous fevers such as West Nile, Rift Valley, Zika, Dengue, Yellow, and Chikungunya among others in different parts of the world. The study area (Nairobi industrial area) neighbors several densely populated informal human settlements. This paper specifically reports on composition and distribution of mosquito species obtained from the study area.Methods: The fourth instars mosquito larvae were collected from waste water channels using the standard dipping method. Adult mosquitoes were trapped using the center for disease control and prevention (CDC) light traps. Purposive random sampling for mosquito adults and larvae was carried out in industrial premises and waste water channels respectively at seven locations. This involved selecting sampling sites from which mosquito samples were likely to be obtained. The mosquitoes were then microscopically identified using taxonomic keys for the Ethiopian and East African region.Results: Out of 2,926 adult mosquitoes trapped, 12 species were identified including Cx. pipiens (95%); Cx. Vansomereni (2.6%); Cx. zombaensis (1.4%); Cx. univittatus (0.34%); Cx. theileri (0.21%); Ae. aegpti (0.14%); An. maculipalpis (0.03%); An. squamosus (0.03%) and other culicid species (0.20%). Of these adult mosquitoes, 94% (2753/2926) were females and 6% (173/2926) were males giving a male: female ratio of 1: 16 when using CDC traps. Of the 4,679 mosquito larvae scooped from the waste water channels, 4 species were identified including Cx. pipiens (99.34%); Cx. vansomereni (0.51%); Toxorhynchites brevipalpis (0.13%) and Aedes mosquito (0.02%).Conclusion: The majority of mosquito species obtained were culicid, Culex pipiens for both adults and larvae. A few Anopheles and Aedes populations were obtained. Unmaintained open waste water channels seen\med to enhance the breeding of urban mosquitoes in the study area. The ecology of these mosquitoes should be studied further to enhance surveillance and controlin order to minimize the risk of mosquito borne viral infections or any other re-emerging mosquito-borne infections to the residents of Nairobi, in particular those living in the informal settlements near Nairobi industrial area, Kenya.Keywords: urban, mosquitoes, Culex pipiens, waste water, industrial area, NairobiAfr J Health Sci. 2017; 30(2):120-13

Modulation of LPS-Induced CD4+ T-Cell Activation and Apoptosis by Antioxidants in Untreated Asymptomatic HIV Infected Participants: An In Vitro Study

Persistent immune activation characterises HIV infection and is associated with depletion of CD4+ T-cells and increased risk of disease progression. Early loss of gut mucosal integrity results in the translocation of microbial products such as lipopolysaccharide (LPS) into the systemic circulation. This is an important source of on-going immune stimulation. The purpose of this study was to determine levels of CD4+ T-cell activation (%CD25 expression) and apoptosis (% annexin V/7-AAD) in asymptomatic, untreated HIV infection at baseline and after stimulation with LPS and incubation with or without vitamin C and N-acetylcysteine. LPS induced a significant ( < 0.03) increase in %CD25 expression, annexin V, and 7-AAD in HIV positive individuals. NAC in combination with vitamin C, significantly ( = 0.0018) reduced activation and early apoptosis of CD4+ T-cells to a greater degree than with either antioxidant alone. Certain combinations of antioxidants could be important in reducing the harmful effects of chronic immune activation and thereby limit CD4+ T-cell depletion. Importantly, we showed that CD4+ T-cells of the HIV positive group responded better to a combination of the antioxidants at this stage than those of the controls. Therefore, appropriate intervention at this asymptomatic stage could rescue the cells before repetitive activation results in the death of CD4+ T-cells

Lay attitudes and misconceptions and their implications for the control of brucellosis in an agro-pastoral community in Kilombero district, Tanzania

Funding: The research for this paper was carried out within the framework of the DELTAS Africa Initiative [Afrique One-ASPIRE /DEL-15-008] (to CMM). Afrique One-ASPIRE is funded by a consortium of donor including the African Academy of Sciences (AAS) Alliance for Accelerating Excellence in Science in Africa (AESA), the New Partnership for Africa’s Development Planning and Coordinating (NEPAD) Agency, the Wellcome Trust [107753/A/15/Z] and the UK government. The authors were funded by Afrique One ASPIRE to conduct this study.Brucellosis is a priority zoonotic disease in Tanzania that causes ill-health in people and affects livestock productivity. Inadequate awareness and behavior risking transmission can impede control efforts. We conducted a cross-sectional survey of 333 livestock owners in three villages in the Kilombero district, Tanzania, to understand their awareness, knowledge and behavior associated with brucellosis. Six Focus Group Discussions (FGDs), two in each village, were conducted, as well as an additional FGD with male herders from one of the villages. Factors associated with knowledge on brucellosis, food consumption and animal husbandry behavior risking transmission of this disease, were identified using generalized linear models. Predictors for knowledge of brucellosis were being male and having a higher educational level, while age was positively associated with a higher level of knowledge. Faith and ethnicity were associated with the performance of practices risking transmission. Following traditional religion and belonging to the Wamaasai ethnicity significantly increased the odds of carrying out these practices. Qualitative analysis gave insight into risk practices and reasoning. Of the 333 respondents, 29% reported that they had experienced abortions in their herds, 14% witnessed retained placentas, and 8% had seen still-births in their cattle within the previous year. However, survey results also showed that only 7.2% of participants had heard about brucellosis as a disease in livestock. Of those who had heard about brucellosis in livestock, 91% associated abortions with it and 71% knew that humans can get infected through raw milk consumption. People overwhelmingly attributed symptoms and transmission of brucellosis in livestock to infection with trypanosomiasis and to supernatural reasons instead. In the community, consumption of raw milk was valued and handling of aborted material was not considered a risk for infection. This agro-pastoralist community holds on to long-held beliefs and practices and lacks understanding of the biomedical concept of brucellosis. Transmission routes and symptoms of brucellosis in humans and livestock are completely unknown. The disparity between risk perception and actual transmission risk related to animal handling and consumption of animal products presents a challenge for disease awareness communication. This study recommends focused community engagement and sensitization to address the limited awareness and misconceptions among agro-pastoralists.Publisher PDFPeer reviewe

Wet market biosecurity reform : three social narratives influence stakeholder responses in Vietnam, Kenya, and the Philippines

Funding: This research was funded by the Australian Agricultural Centre for International Agricultural Research (ACIAR), grant number LS/2020/204. The funders played no role in the design or implementation of the research. Grant recipients included: KB, RG, SB, TH, CM. All authors received salary support from the grant.In 2020, Covid-19 led to global policy statements promoting bans and reforms to wet markets in Asia and Africa to prevent future pandemics. We conducted a comparative, exploratory qualitative study in 2021 in three countries (Kenya, Vietnam and the Philippines) to understand the social and political dimensions to biosecurity reform at wet markets. This included 60 key informant interviews and rapid ethnographic research in 15 markets, as well as a review of policy documents and online media articles. We found no evidence that the rhetoric of pandemic spillover that emerged in 2020 had any influence on policy or reform efforts apart from those related to Covid-19 infection control. Rather, we identified three main narratives that frame the problem of biosecurity and preferences for reform. The first, a human health narrative, questioned global framings about pandemic risk, viewed markets as sources for food security rather than disease, emphasized the need to strengthen the control of endemic diseases, and conceptualized health through the lens of ‘freshness’ rather than biomedical categories. A second modernization narrative approached biosecurity as part of a broader process of socio-economic development that emphasized infrastructural gaps, spatial arrangements, cleanliness and a conflict between reform and economic interests. A third narrative centered on local livelihoods and the tension between local market stakeholders and biosecurity and modernization efforts. This final narrative called into question the appropriateness of certain regulations and policies, including bans and closures, emphasized the importance of preserving cultural heritage and highlighted the need for collective political action to resist certain veterinary policies. In conclusion, wet market biosecurity strategies occur in the context of three contrasting narratives that emphasize different aspects of health and risk, and reflect different worldviews and interests. Within this context, there is a need for local government to strengthen market management and biosecurity in ways that enhance the agency of market stakeholders and strengthen local livelihoods and food security as part of a pluralistic and democratic politics.Publisher PDFPeer reviewe

Returns to research and outreach for integrated pest management of western flower thrips infesting French bean and tomato in Kenya

Thrips, Frankliniella occidentalis (Pergande), is a major invasive pest that causes extensive yield losses in French bean and tomato in Kenya. Thrips management is based on the application of pesticides. In addition to increased environmental risks associated with pesticides, frequent use of these chemicals increases production costs and pesticide resistance. Furthermore, exports are restricted due to non-compliance to maximum residue levels in important consumer export markets, especially the European Union (EU). This study was conducted to estimate the potential benefits of the effectiveness of the icipe-developed strategy for control of western flower thrips before dissemination of the technology in Kenya, using the economic surplus model. We calculated the benefit–cost ratio, the Net Present Value (NPV) and the Internal Rate of Return (IRR) using Cost–Benefit Analysis (CBA). Assuming a maximum conservative adoption rate of 1% and a 10% discount rate for the base deterministic scenario, the NPV of the research was estimated at US$2.2 million, with an IRR of 23% and a BCR of 2.46. Sensitivity analyses indicated that the NPV, IRR and BCR increased at an increasing rate as adoption rates increased. However, as elasticities of supply and demand increased, the NPV, IRR and BCR increased at a decreasing rate. The findings demonstrate that farmers from developing countries can gain when they obtain access to suitable pest management innovations such as integrated pest management technologies. Consequently, investment in IPM technologies for suppression of western flower thrips should be enhanced

Assessing food security among young farmers in Africa: evidence from Kenya, Nigeria, and Uganda

Open Access JournalFood insecurity remains a serious challenge for many households in Africa and the situation is even more prevalent among young people. However, there is a dearth of empirical evidence on youth food security status in Africa. We assessed the level and determinants of food security among young farmers in Africa. We adopted a multi-stage sampling technique to select 400, 429, and 606 young farmers in Kenya, Nigeria, and Uganda, respectively. Individual food consumption was assessed following a 7 days recall method. The Food Consumption Score, which combines dietary diversity and consumption frequency was used to assess food security status while the determinants of food security were identified using a logistic regression model. Results suggest low dietary diversity across the three countries. Also, the majority of the respondents had an unacceptable food consumption score, suggesting that despite being food producers, young farmers are still food insecure. The odds of being food secure was positively determined by access to extension services, participation in the ENABLE TAAT business incubation programme, and access to market information but, negatively by access to credit, number of employees, Covid-19 pandemic, and location. Additionally, the food security status of young female farmers was positively influenced by age, suggesting that younger youths are less food secure compared to older ones. These results suggest that more efforts should be directed towards improving the food security of young African farmers and that policy- and programme-level interventions should support access to extension services, market information, and land. Additionally, more investments should be directed towards developing need-based agribusiness incubation programmes with an effort to scale existing programmes beyond the regular one-time period