研究HIMSS EMRAM 7级评审在住院药房中的应用

目的探讨美国医疗信息与管理系统学会电子病历应用模型(Healthcare Information and Management Systems Society Electronic Medical Record Adoption Model,HIMSS EMRAM)7级评审在住院药房工作中的应用价值。方法回顾性统计厦门大学附属第一医院住院药房2016年10月1日—2017年7月31日HIMSS EMRAM 7级评审前每条医嘱(非包药机摆药医嘱,以下简称医嘱)平均调剂时间、人力成本、医嘱调配差错率、耗材使用量等指标,与2017年9月1日—2018年6月30日HIMSS EMRAM 7级评审后的相应指标进行比较分析。结果 (1)评审后每条医嘱平均调剂时间为(19.24±0.31)s,低于评审前的(29.73±0.56)s(t=244.73,P <0.01)。(2)同样调剂1 642 867条医嘱,评审后比评审前节省人员2.75人,节省人力成本137 500元。(3)评审后的医嘱调配差错率为1.35‰(2 219/1 642 867),低于评审前的2.13‰(3 455/1 625 694)(χ2=282.89,P <0.01)。(4)评审后打印纸消耗、打印机维保及打印机损耗等耗材使用量仅为评审前的5.14%,可节约43 597.67元。结论 HIMSS EMRAM 7级评审过程,通过对住院药房进行信息化管理,缩短了医嘱平均调剂时间、减少了医嘱调配差错率、节约人力成本、减少耗材消耗,是实现药品安全质量管理目标的有效措施

Optimization of mixed fermentation technology of Lactobacillus helveticus andLactobacillus rhamnosus based on high viable count

以瑞士乳杆菌和鼠李糖乳杆菌混合发酵培养,利用两菌株细胞代谢的差异,以静置发酵24 h后得到的活菌数目为指标,通过单因素实验和正交实验,研究了瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最适发酵条件。结果表明,瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最优条件为:发酵温度37℃,初始pH=6.8,接种量6%,瑞士乳杆菌:鼠李糖乳杆菌=1∶2,瑞士乳杆菌优先接种3 h。最终得到乳酸菌总活菌数为7.2×10~9m L~(-1)。与在相同条件下单独发酵的瑞士乳杆菌和鼠李糖乳杆菌活菌数相比,分别提高了1∶8倍和10.2倍。为乳酸菌的高活菌数发酵奠定了基础

人死亡受体5全长基因的构建及转染胶质瘤细胞

目的构建人死亡受体5(death receptor5,DR5)全长基因真核细胞表达载体pcDNA3.1/DR5,pcDNA3.1/GFP/DR5,并观察其转染胶质瘤细胞U138的效果。方法通过重叠PCR获得DR5全长编码序列,构建pcDNA3.1/GFP/DR5,pcDNA3.1/DR5表达载体,利用脂质体转染试剂盒,分别将2种质粒pcDNA3.1/GFP/DR5、pcDNA3.1/GFP共转染胶质瘤细胞U138,转染后72h,半定量逆转录聚合酶链反应(RT-PCR)检测DR5mRNA的表达,流式细胞术检测DR5的表达强度、检测Anti-DR5对胶质瘤细胞U138生长的影响。结果获得了DR5全长编码序列,成功瞬时转染U138,RT-PCR、流式细胞术检测结果表明,转染后U138细胞DR5mRNA、蛋白水平的表达明显增加,Anti-DR5可以明显抑制U138细胞的生长。结论获得了DR5全长编码序列,探索到成功转染DR5的最佳方法,为稳定筛选高表达DR5的U138细胞提供依据

Analysis of DcR3 in rat model of adjuvant arthritis

目的研究诱骗受体DcR3对佐剂型关节炎(AA)大鼠模型的作用及其机理。方法注射弗式完全佐剂建立大鼠佐剂型关节炎(AA)模型,尾静脉注射DcR3蛋白,观察大鼠关节肿胀度、间接ELISA检测血清和滑膜液中细胞因子IL-1β、TNF-α、IFN-γ的变化。RT-PCR检测滑膜和淋巴细胞中DcR3、Fas、FasL mRNA的表达以及脾脏中TGF-β、IFN-γ、TNF-α、IL-4、IL-10 mRNA的表达。Western blot分析滑膜细胞中Caspase-8、Caspase-3、Caspase-9、Bcl-2蛋白的表达。结果DcR3治疗AA大鼠后,足肿胀度降低;血液和滑膜液的IL-1β、TNF-α、IFN-γ水平下降;脾脏中TGF-β、IFN-γ、TNF-αmRNA表达下调,IL-4、IL-10 mRNA表达上调;滑膜细胞中Caspase-8、Caspase-3蛋白表达上调,Bcl-2蛋白表达下调。结论DcR3可以用于实验性大鼠AA的治疗,其治疗机制与调节滑膜细胞FasL、Fas mRNA的表达和血液淋巴细胞中Fas mRNA的表达,促进滑膜细胞和自身反应性淋巴细胞的凋亡;调节脾脏细胞Th1/Th2细胞因子... 【英文摘要】 Objective To study the immunoregulatory mechanisms of DcR3 on rats adjuvant arthritis(AA).Methods AA was induced by complete freund s adjuvant(CFA)in rats,and the rats were injected subcutaneously with DcR3.The perimeters of rat hind soles were measured before and after the injection of CFA.The pathological changes of inflammatory knee joints were observed under optical microscope.The changes of IL-1β,TNF-α,and IFN-γ in serum and synovial liquid were detected by indirect ELISA,respectively.The DcR3,Fas,and ...厦门大学科研启动基金(Z03103

Key bacteria during microbial degradation of pyrene

[Objective] This study aimed to identify the key bacteria during the microbial degradation of pyrene in the sediments from Bohai sea and the potential interactions among these bacteria. [Methods] We set up the microcosms system with pyrene as the sole carbon source, apllied the Illumina Hiseq 2500 to reveal the bacterial communities, and then predicted the bacterial ecological interactions using CCLasso algorithm. [Results] The concentration of pyrene decreased by (67.072.37)% after 30 days and meanwhile the structures of bacterial communities were distinctly changed. The significantly enriched population consisted of Alphaproteobacteria, Flavobacteriia and Planctomycetia, whereas the relative abundances of Deltaproteobacteria, Anaerolineae and Spirochaetes decreased. The microbial ecological network was constructed and composed of 29 nodes and 143 edges. The classified genera with relatively high degree values included Erythrobacter and Planctomyces. The strong associations were observed between the genus Erythrobacter and some unclassified genera affiliated to the family Flavobacteriaceae and the class Alphaproteobacteria. [Conclusion] It is possible to address scientific questions from the classic ecology to identify the key bacteria during the biodegradation of polycyclic aromatic hydrocarbons compounds by marine microbial ecological networks. Our study discovered close interactions among key bacteria represented by the genera Erythrobacter

Structure characteristics of core bacterial communities in surface sediments and analysis on their responses to environmental factors in the inlet of Bohai Bay

[Background] In recent years, the increasingly polluted environment of Bohai Bay has impacted the sustainability of offshore ecosystems. [Objective] To explore the distribution characteristics of bacterial communities in surface sediments and their response to environmental factors, we selected surface sediment samples from 21 stations for study. [Methods] We investigated the microbial community structure by sequencing the V3-V4 hypervariable regions of bacterial 16S rRNA gene using the Illumina HiSeq 2500. Bacterial community composition, spatial distribution characteristics and environmental factors were combined and analyzed in an attempt to understand the key interactions. [Results] The 16S rRNA gene clone library analysis indicated that the most common phyla that were widely distributed in the 21 sites were: Proteobacteria (56.8%), Acidobacteria (8.9%), Chloroflexi (8.1%), Bacteroidetes (6.2%). Moreover, γ- Proteobacteria and δ- Proteobacteria were the most dominant classes in Proteobacteria. Total organic carbon and total organic nitrogen content in the near shore sediments is high due to the input of anthropogenic materia and gradually decreased offshore. The most significant influence on species composition and community structure within the study area were found to be from particle size (content<4 μm) and organic nitrogen load. [Conclusion] The microbial diversity in the inlet of Bohai Bay is very high and is significantly related to a variety of environmental parameters. Human activities play an important role in structuring the microbial diversity, community structure and distribution

一种混合乳酸菌发酵方法

一种混合乳酸菌发酵方法，包括如下步骤：将瑞士乳杆菌和鼠李糖乳杆菌菌种分别进行活化处理，得到瑞士乳杆菌种子液和鼠李糖乳杆菌种子液；将瑞士乳杆菌种子液中的瑞士乳杆菌和鼠李糖乳杆菌种子液中的鼠李糖乳杆菌接种至改良的番茄汁肉汤培养基中，发酵22h-24h，其中，瑞士乳杆菌和鼠李糖乳杆菌的体积比为1:1-1:2，接种量为5%-7%，改良的番茄汁肉汤培养基的初始pH值为6.6-7.0，发酵温度为30℃-37℃，得到发酵后的混合乳酸菌。上述混合乳酸菌发酵方法，瑞士乳杆菌有较强的蛋白水解能力，可将培养基中的大分子蛋白水解成小分子，给共同发酵的鼠李糖乳杆菌提供一定的小分子蛋白，从而充分利用培养基中的营养成分，从而同时获得较多的瑞士乳杆菌和鼠李糖乳杆菌的活菌数

Optimization of mixed fermentation technology of LactobaciUus helveticus and LactobaciUus rhamnosus based on high viable count

以瑞士乳杆菌和鼠李糖乳杆菌混合发酵培养，利用两菌株细胞代谢的差异，以静置发酵24h后得到的活菌数目为指标，通过单因素实验和正交实验，研究了瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最适发酵条件。结果表明，瑞士乳杆菌和鼠李糖乳杆菌高活菌数混合发酵的最优条件为：3K酵温度37oC，初始pH=6．8，接种量6％，瑞士乳杆菌：鼠李糖乳杆菌=1：2，瑞士乳杆菌优先接种3h。最终得到乳酸菌总活菌数为7．2x10。mL。与在相同条件下单独发酵的瑞士乳杆菌和鼠李糖乳杆菌活菌数相比，分别提高了1：8倍和10．2倍。为乳酸菌的高活菌数发酵奠定了基础