Probing Hydroxyl Radical by Fluorescence

提出了fEnTOn - 荧光探针表征·OH 的新方法， 并以吖啶黄为荧光探针研究了此方法的光谱及动力学特征. 在最佳反应条件下， 对多种·OH 清除剂的能力进行了表征.In this article,we present a new method for probing the generation or scavenging of hydroxyl radical.The method is based on the generation of hydroxyl radical by Fenton reaction and the determination of hydroxyl radical by fluorescence.The spectral and dynamic characteristics of the probing system were investigated.福建省自然科学基金!（D9920001

五氯化磷辅助下氨基酸的自组装成肽反应研究

L α 氨基酸和D α 氨基酸可与五氯化磷直接发生磷酰化反应 ,随后自组装成多肽 ,但 β 氨基酸不能成肽 ,DL α 氨基酸成肽困难 ;在SOCl2 存在下 ,α 氨基酸也不能成肽 .用电喷雾质谱研究了氨基酸的自组装反应 .反应过程中有五元环状的氨基酸五配位磷中间体生成 ,使用硅烷基保护的氨基酸 ,在 3 1PNMR中可观察到五配位磷中间体

献血者中戊型肝炎病毒血症研究

目的　了解湖州地区献血者戊型肝炎病毒感染情况。方法　采用ELISA法检测 30 4 7名无偿献血者血清抗 HEVIgM和IgG抗体 ,巢式RT PCR检测抗 HEVIgM阳性血清中HEVRNA。 结果　 30 4 7名无偿献血者中抗 HEVIgG的检出率为 4 1.7% ,抗 HEVIgM检出率为 1.5 %。 4 6份抗 HEVIgM阳性中发现 6份HEVRNA阳性。献血者中病毒血症阳性率为 0 .2 %。 6名HEV病毒血症者中 4名为HEV基因Ⅰ型 ,另 2例为HEV基因Ⅳ型。结论　在献血者中HEV病毒血症并不罕见 ,有必要对输血后戊肝的潜在危险进行评估

Zooplanktonic diversity in the western Pacific

西太平洋区是全球海洋生物种源中心,许多类群的最高物种多样性都出现于该区域,因此,在该区开展种类多样性的研究不仅重要和必要,而且具有在跨国尺度上进行综合管理和相互合作的迫切性。本文在西太平洋的浮游动物样品鉴定分类、编目、文献资料整理和分析的基础上,记录和编入西太平洋10个浮游动物类群2,658种(含亚种),隶属于206科675属,其中水螅水母类99科251属697种,栉水母类12科22属59种,浮游软体动物14科35属86种,介形类8科89属416种,桡足类51科156属908种,糠虾类4科58属202种,磷虾类2科8属56种,十足类8科22属105种,毛颚类5科8属48种,被囊类5科26属81种。The western Pacific region has been operating as a centre for the origin of marine biodiversity: the richest diversity of many marine taxa was found in these waters.Therefore,biodiversity research and con-servation efforts in this area are necessary in order to promote the integrative and international management of this resource.The present work is a compilation of numbers of all the families,genera and species of ma-jor taxa of zooplankton known in the western Pacific Ocean(106°–150°E,0°–44°N).In all,2,658 zooplank-tonic species(including subspecies) belonging to 206 families and 675 genera have been recorded from taxonomic identifications and literature,99 families,251 genera and 697 species belong to the Medusozoa,12 families,22 genera and 59 species to the Ctenophora,14 families,35 genera and 86 species to the pelagic Molluscs(Pteropoda and Heteropoda),8 families,89 genera and 416 species to the Ostracoda,51 families,156 genera and 908 species to the Copepoda,4 families,58 genera and 202 species to the Mysidcea,2 fami-lies,8 genera and 56 species to the Euphausiacea,8 families,22 genera and 105 species to the Decapoda,5 families,8 genera and 48 species to the Chaetognatha,5 families,26 genera and 81 species to the Tunicata.908专项“中国海洋生物种类名录和图谱”(908-ZC-II-02)和908专项海洋生物样品库;科技部基础工作专项“我国和邻近西北太平洋海洋生物物种编目和分布图集编制”(2006FY220700

Clover探测器的极化测量

介绍了利用Colver探测器测量γ射线的线性极化方法.用~(60)Co源对其进行测试,有效地测量了γ射线的电磁属性,从而确定了能级的宇称

双奇核~(174)Re中的准粒子形状驱动效应

对双奇核174Re的在束γ谱学实验数据作了仔细分析,提取出了带间与带内E2γ跃迁分支比,并导出了其约化跃迁概率的比值。用带混合理论对约化跃迁概率的比值进行了分析,得到带间相互作用强度及转动带形变的信息。发现不同组态的转动带其跃迁电四极矩具有明显的差异。与相邻奇A核的结构特征类似,这种跃迁电四极矩的差异很可能是由于1/2-[541]准质子或1/2-[521]准中子对原子核的形状驱动效应造成的

Studies on the Fluorometric Method for Determining Activity of Catalase and Its Application to Marine Biosamples

建立了一种简便、灵敏的测定过氧化氢酶的方法 .方法的线性范围为 1 .7× 1 0 - 3～ 1 .7× 1 0 - 2 U/m L,检测限 (LD=3 S0 /S)为 8.5× 1 0 - 4U/m L.将其用于海洋生物样品中过氧化氢酶活力的测定 ,结果令人满意A new promising method for determining activity of catalase and its application are presented. The measurement of catalase activity is based on the inhibition of catalase on Fenton reaction. Catalase catalyzes the dissociation of H 2O 2, one of the main reactants of Fenton reaction which yields ·OH stoichiometrically, and restrains the increment of the fluorescence intensity, produced as a result of quinoline hydroxylation. The decrease of fluorescence intensity was linearly related to the activity of catalase and the linear range covered from 1 7×10 -3 U/mL to 1 7×10 -2 U/mL. A detection limit of 8 5×10 -4 U/mL catalase was obtained. This method has been applied to the detection of catalase in marine biosamples and satisfactory results were obtained.福建省自然科学基金 (批准号 :D992 0 0 0 1)资