DDEFL1的结构和功能研究进展

DDEFL1又称为ASAP3(、ACAP4或UPLC1,属于小G蛋白家族中Arfs家族的一种ArfGAP。人的肝和肺是DDEFL1表达水平较高的两个组织[1]。了解DDEFL1的结构及功能,可以为肝癌或其它癌症患者提供一种新的基因治疗方案

Detection of 2-Deoxyglucose by Pre-column Derivation Reversed Phase High Performance Liquid Chromatography

采用对氨基苯甲酸乙酯为衍生化试剂,建立柱前衍生化反相高效液相色谱法(rP-HPlC)测定2-去氧葡萄糖的含量。结果表明,最适的衍生化条件为反应温度85℃,反应时间1.5H,衍生化试剂(AbEE)和单糖(2-dg/glu)的摩尔比为25∶1;色谱条件为色谱柱HyPErSIl OdS2(250MMx4.6MM,5μM),流动相为0.02%TfA-乙腈(80∶20,V/V),流速1.0Ml/MIn,波长307nM。本方法在1~700Mg/l浓度范围内与峰面积呈良好的线性关系(r=0.9999);定量限(S/n=10)及检出限(S/n=3)分别为450和100μg/l;平均加标回收率为101.7%;rSd为0.7%;日内及日间精密度均小于0.7%。表明本方法灵敏度高,测定结果准确,重复性好,可用于2-去氧葡萄糖样品的准确定量分析。A method was developed for the determination of 2-deoxyglucose(2-DG)by reversed phase high performance liquid chromatography(RP-HPLC) after pre-column derivated with p-aminobenzoic ethyl ester(ABEE).The results showed that the optimum derivation conditions were as follows: reaction temperature was 85 ℃,reaction time was 1.5 h and molar ratio of derivative reagent(ABEE) to monosaccharide(2-DG/Glu) was 25∶1.The derivative was analyzed on a Hypersil ODS 2(250 mm×4.6 mm,5 μ) column with a mixture of acetonitrile to 0.02% trifluoroacetic acid(TFA)(2∶80,V/V) as the mobile phase,at the flow rate of 1.0 mL/min and the detection wavelength at 307 nm.This method has a good linearity at the concentration from 1 mg/L to 700 mg/L(r=0.9999).The limit of detection was 100 μg/L while the quantitative limit was 450 μg/L.The average recovery of 2-DG was 101.7% and the relative standard deviation(RSD) was 0.7%.The inter-day and intra-day precision were both less than 0.7%,This method is of high sensitivity,precision and repetition,indicating that it is suitable for precise quantitative determination of 2-DG.海洋公益性行业科研专项经费基金(No.201005020);福建省科技重大专项经费基金(No.2010NZ0001-2);厦门市海洋与渔业局科技计划项目经费基金(厦海渔合[2010]9号)资助项

大连蛇岛蝮蛇类凝血酶基因克隆与表达研究

根据同源性设计引物，通过RT－PCR方法从大连蛇岛蝮蛇毒腺总RNA中合成扩增出类凝血酶基因，并由此推测出其相应的氨基酸序列。将该基因克隆到表达载体pPIC9K中，经电激转化后整合至毕氏酵母细胞中获得表达。该表达产物经两步柱层析后，进行聚丙烯酰胺凝胶电泳，获得单一条带，并表现较强的生色底物活性

大连蛇岛蝮蛇类凝血酶基因克隆与表达研究

根据同源性设计引物,通过RT-PCR方法从大连蛇岛蝮蛇毒腺总RNA中合成扩增出类凝血酶基因,并由此推测出其相应的氨基酸序列.将该基因克隆到表达载体pPIC9K中,经电激转化后整合至毕氏酵母细胞中获得表达.该表达产物经两步柱层析后,进行聚丙烯酰胺凝胶电泳,获得单一条带,并表现较强的生色底物活性

河南省鸟类新纪录烟腹毛脚燕

2016年6月22日,在河南省南阳市内乡县夏馆镇葛条爬村发现烟腹毛脚燕,此次发现为烟腹毛脚燕在河南省鸟类新纪录。本文报道了其形态特征,并对该物种的分布状况进行了讨论

循环流化床热解油田采油污泥的实验研究[J]

以新疆克拉玛依油田采油污泥为对象,将煤与其混合后考察了混合物的失水情况,并采用热重分析仪和小型循环流化床热解反应器研究了混合物的热解过程及其热解效果.结果表明,与煤混合后油泥的干燥速度明显加快,干燥后的混合物90％以上粒径分布在1～6 mm范围内.混合物热解主要经历了轻质组分挥发、重组分快速热解失重和缓慢失重3个阶段.流化床热解结果表明,在600℃下热解3 min时,油、气总产率最高