Study on Fast Sample Preparation Techniques for Pesticide Residue Analysis in Teas and Vegetables

Abstract Solid-phase microextraction (SPME) is a novel environment-friendly sample preparation technology developed in 90’s last century and evolving rapidly. The technique needs no organic solvents and the operation is simple and convenient. SPME combines extraction, concentration and sample injection procedures into one single step. SPME was developed from solid-phase extraction (SPE) te...学位：理学硕士院系专业：化学化工学院化学系_分析化学学号：20022503

On the Inner_Party Inspection Policy of Communist Party of China

中国共产党的巡视工作是在监督理论的指引下，结合我国历史的和现实的经验而形成的一种加强党内监督、推进反腐倡廉工作的重要制度安排。长期以来，对党员领导干部的有效监督和科学管理，是中国共产党党员干部监督管理体制中相对薄弱的环节之一。中国共产党成立后不久，就在党内尝试开展巡视工作，有力地推进了早期党的组织建设，使党的组织不断地发展和壮大。中国共产党执政以来，特别是党的十六大以来，针对新形势下党的建设特别是反腐倡廉建设中出现的新情况、新问题，中共中央把建立党内巡视工作制度作为完善党内监督机制、加强党内监督的一项重大举措，取得了明显成效。该制度的施行在一定程度上克服了上级党委、纪委与下级党组织领导干部的“...Combined with China's historical and realistic experiences, the inspection of the Communist Party of China has developed into a kind of strengthened inner-party supervision under the guidance of the Monitoring Theory. There has been for a long time in want of an effective and scientific system of supervision and management over party’s leading cadres as well as party’s members. Soon after its esta...学位：公共管理硕士院系专业：公共事务学院_公共管理硕士(MPA)学号：X200913619

树立环境价值观,引导可持续发展

树立环境价值观是解决环境生态问题、实现可持续发展的根本性对策。本文简述了目前关于环境价值观理论分析的一些主要研究结果,以及环境价值与马克思主义劳动价值论的关系,在此分析基础上指出,开展环境价值研究,尤其是对环境价值的量化方法和将环境核算纳入国民经济核算体系的途径的研究,对推动我国可持续发展具有重要的现实意义

慢性乙型肝炎潜在治疗靶点和新药研发进展

尽管预防性疫苗显著减少了乙型肝炎病毒(hepatitis B virus,HBV)新发感染,但目前全球仍有超过2.4亿慢性HBV感染者,其中每年因HBV感染相关的终末肝病和肝癌引起的死亡人数高达68万。目前用于慢性乙型肝炎(chronic hepatitis B,CHB)治疗的抗病毒药物包括干扰素和核苷/核苷酸类似物两大类,但均难以实现理想的临床治疗终点,即乙肝表面抗原(HBsAg)阴转或血清学转换。针对CHB患者尚未被满足的巨大医疗需求,国内外团队正在针对HBV生活周期的各个关键步骤以及潜在的宿主因子,尝试研发更为有效的CHB治疗药物,本文简要综述了当前处于临床开发阶段以及部分临床前阶段的CHB候选药物研发进展

Preparation of inorganic coagulants from waste stone powder and its effects of turbidity removal

利用废石粉渣制备无机混凝剂，并研究所得初产品对硅藻土悬浮液和受污湖水样品进行处理的适宜操 作条件以及除浊净水效果。结果表明，利用某些废石粉值制备的混凝剂有较好效果，但制备工艺有待改善。In order to recover coagulation-effective substances from waste stone powder, some inorganic coagulants were prepared from waste stone powder.Then the effects of the prepared coagulants on turbidity removal from polluted lake water and diatomile suspended solution were tested, some parameters, such as dosage and pH, were also studied.The results showed that the coagulants had good effects on turbidity removal.厦门市夕阳红科学基

Progress on development and application of coagulants and flocculent in water and wastewater treatment

分三部分论述了 90年代以来国内外混凝剂和絮凝剂研究的主要进展 :1 )混凝、絮凝的微观机理及其行为的研究成果和实验研究手段 ;2 )混凝剂和絮凝剂的开发 ;3)混凝和絮凝方法在废水处理中的净化效率的提高、条件优化和在线监控等。s:The recent development of coagulants and flocculent was reviewed.Three topics were discussed:(1)Mechanisms of coagulants and flocculent,and the experimental methods of process study;(2)Development of coagulants and flocculent;(3)Researches on improvement of coagulants and flocculent efficiency,process optimization and on line monitoring and controlling as well.厦门市科委夕阳红基金会资

乙型肝炎病毒体外感染和复制的细胞模型

慢性乙型肝炎病毒(Hepatitis B virus,HBV)感染是严重威胁人类生命健康的世界性公共卫生问题。基于现有抗HBV药物的治疗策略,仅能在极少部分患者中实现慢性乙肝的功能性治愈。发展更为有效的抗HBV药物,需要更加透彻全面地认识各个病毒组分和关键宿主因子在HBV感染和复制生命周期中发挥的功能和机制,并在此基础上发现鉴定新的治疗靶点。支持HBV体外感染和复制的细胞模型,是研究HBV生活史的重要工具,并在治疗新靶点的发现和候选药物功效评估等研究工作中发挥关键作用。本文对支持HBV感染和复制细胞模型的新近研究进展进行梳理分析,并对这些模型的应用特点和局限性、新近研究进展和未来发展方向进行系统阐述和讨论。国家自然科学基金(81672023)~

细胞穿膜肽研究应用的新进展

细胞穿膜肽(Cell-penetrating peptides,CPPs)是一类能够穿过细胞膜或组织屏障的短肽。CPPs可通过内吞和直接穿透等机制运载蛋白质、RNA、DNA等生物大分子进入细胞内发挥其效应功能。相比于其他非天然的化学分子,CPPs具有生物相容性佳、对细胞造成的毒性小、完成入胞转运后可降解、并能与生物活性蛋白直接融合重组表达等优点,因此成为以胞内分子为靶标的药物递送技术发展的重要工具,并在生物医学研究领域具有良好的应用前景。文中针对CPPs的分类特点、入胞转运机制及其治疗应用的新近研究进展进行综述和讨论。国家自然科学基金(No.81672023)资助~

Simultaneous Determination of Residues of Organochlorine and Pyrethroid Pesticides in Tea by Microwave Assisted Extraction Solid Phase Microextraction-Gas Chromatography

建立了微波辅助萃取-固相微萃取-气相色谱(MAE-SPME-GC)同时测定茶叶中六六六(α-BHC,β-BHC,γ-BHC,δ-BHC 4种异构体)、滴滴涕类(DDD,DDE,o,p′-DDT,p,p′-DDT)、氯氰菊酯(cypermethrin)和氰戊菊酯(fen-valerate)等10种农药残留的方法。采用外标法定量,除氰戊菊酯外,农药的质量浓度与其色谱峰面积在一定范围内有较好的线性关系,相关系数为0.970 5~0.998 4。10种组分的加标回收率为64%~121%,相对标准偏差为10.4%~22.9%,检测限为1~50 ng/L。应用该方法测定了市场上3种茶叶中上述农药残留的含量。The issue of residues including organochlorine and pyrethroid pesticides in tea has made a great attention of researchers and customers in the world.Although some analytical methods have well been established to detect these pesticides,the sample preparations are generally laborious,time-consuming,and some of the procedures caused environmental pollution.Solid phase microextraction(SPME) is a novel sample preparation technique since it is of simplicity,high efficiency and solvent free.SPME was firstly developed to address the need to facilitate environment monitoring.In recent years,great efforts have been made in the analysis of pesticide residues in agricultural products by SPME.In this research,a microwave assisted extraction solid phase microextraction-gas chromatography(MAE-SPME-GC) was applied for the simultaneous determination of organochlorine and pyrethroid pesticides,such as benzene hexa chlorides(α-BHC,β-BHC,γ-BHC and δ-BHC),1-(2-chlorophenyl)-1-(4-chlorophenyl)-2,2,2-trichloroethane(o,p′-DDT),2,2-bis(4-chlorophenyl)-1,1-dichloroethylene(DDE),2,2-bis(4-chlorophenyl)-1,1-dichloroethane(DDD),2-(2-chlorophenyl)-2-(4-chlorophenyl)-1,1,1-trichloroethane(p,p′-DDT),cypermethrin and fenvalerate.All the tested pesticides were baseline separated under the experimental conditions,except for DDD and o,p′-DDT.The concentration of each pesticide was calibrated by external standard method.The linearities were found to be in the correlation coefficient range of 0.9705 to 0.9984 for the tested pesticides between the mass concentrations and peak areas,except for fenvalerate.The recoveries,relative standard deviations and detection limits for the 10 pesticides were 64%-121%,10.4%-(22.9%,) and 1-50 ng/L,respectively.The proposed method was successfully applied to the determination of organochlorine pesticide residues in three kinds of teas from the local market.The experimental results demonstrated that MAE-SPME-GC was a fast,sensitive and green technique for the analysis of trace quantity of pesticides in solid substrate foods.福建省科技计划项目(2005I-030,2006Y0026

一种联合检测乙型肝炎病毒前S1抗原与核心抗原方法的建立及其与病毒核酸检测结果的一致性

本研究以与血清中HBV DNA含量高度相关的两种HBV抗原(前S1抗原与核心抗原)为靶标,建立了联合检测这两种HBV核酸相关抗原(NRAg)的双抗体夹心法ELISA试剂。对系列稀释血清的检测表明,该试剂的平均分析灵敏度为103.2基因组拷贝/mL(95%可信限102.2-4.2基因组拷贝/mL),显著高于前S1抗原或核心抗原的单独检测。对994份HBsAg阴性血清的检测结果表明NRAg ELISA的特异性为99.7%(95%可信限:99.1%~99.9%)。对271份临床慢性肝炎血清进行检测,结果NRAg ELISA与HBV DNA结果的总符合率达96.3%(95%可信限:93.3%~98.2%),NRAg ELISA的读值/临界值比(S/CO)与HBV基因组拷贝数呈正相关。利用NRAg试剂,发现了1例HBsAg"a"抗原表位突变的变异株。这些结果显示HBV NRAg ELISA与HBV DNA具有高度相关性,并能够检测出HBsAg抗原变异株,有望成为HBsAg变异株筛选的有力工具,并为广大基层医疗单位提供一种便捷的替代HBV DNA定性检测的手段