Effects of Medium on Sporification of Fusarium oxysporum Schl.

选择8种培养基,采用液体培养法,研究培养基质对尖孢镰刀菌(Fusarium oxysporum Schl.)281菌株小孢子产生量的影响,结果表明,在PDA培养基上培养144h得到最大小孢子产量1.283×109cfu/ml,与其它培养基产孢量差异显著(P=0.05)。培养至192h时,ATCC培养基的小孢子数量达到1.325×109cfu/ml,与PDA培养基之间无显著差异(P=0.05),与其它6种培养基之间差异显著(P=0.05)。综合比较8种培养基,PDA培养基培养时间短,配制简单,是较为理想的产小孢子培养基。The results of eight mediums effect on sporification of Fusarium oxysporum 281 showed that the number of sporification is 1.283×109cfu/ml in PDA medium after 144h,Significant difference were observed between PDA medium and others(P=0.05).The number of sporification is 1.325×109cfu/ml in ATCC medium after 192h,there are no significant difference between ATCC and PDA medium(P=0.05).PDA medium is suitable for sporification.福建省自然科学基金“内生生防菌短芽孢杆菌GFP标记及其对黄瓜枯萎病生防机理的研究”(2006J0068);; 福建省发改委重点项目“生物农药高毒力菌株筛选利用与菌种基因资源库的建立”(闽发改投资[2006]781号

Fingerprint analysis on methyl fatty acid and its applications in microbial study

脂肪酸甲酯谱图分析方法(Fatty Acid Methyl Ester,FAME)是鉴于脂肪酸可作为生物标记物而发展起来的分析技术。本文介绍了FAME谱图分析方法及其在微生物学领域的应用,包括在微生物检测、鉴定和微生物多样性研究中的应用。Fatty acid is an important biomarker.Its application in biology research has become increasingly popular.The method of FAME fingerprint analysis is based on fatty acid's utilization as a biomarker.This paper describes the FAME fingerprint analysis and its applications for microbial studies,including identification of unknown microbes and assessment of microbial diversity.国家863计划项目(2006AA10A211);; 福建省发展和改革委员会重点项目[闽农产(2006)10号];; 福建省青年科技人才创新项目(2003.J046

Researches on Urban Compactness Based on Grid Method： A Case Study of Tianhe District, Guangzhou

High-resolution remote sensing data of Guangzhou Tianhe district is used to extract building by object-oriented method. Then, the extracted information is used as one of basic data to identify an assessment indices system for measuring urban compactness

基于情景分析的广东省建筑节能减排潜力研究

Energy consumption and greenhouse gas（GHG）emissions in construction sector of Guangdong Province in 2020 were predicted and the effects of different policy options on them were analyzed by the scenario analysis method aiming at finding the way to energy-

Analysis and Dynamic Prediction of Building Energy Consumption and GHG Emission in Guangdong Province Based on Scenario Analysis Method

Energy consumption and greenhouse gas（GHG）emissions in construction sector of Guangdong Province in 2020 were predicted and the effects of different policy options on them were analyzed by the scenario analysis method aiming at finding the way to energy-

Impact of Urban form on Private Travel Energy Use of Typical City in China

In this paper, panel data from 2000 to 2010 of five typical city in China used to analyze the effect of population density on private travel energy use. And then, the STIRPAT model was created to estimate the effect of urban form and residents＇income on

基于格网法的城市空间紧凑度研究以广州市天河区为例

High-resolution remote sensing data of Guangzhou Tianhe district is used to extract building by object-oriented method. Then, the extracted information is used as one of basic data to identify an assessment indices system for measuring urban compactness

A Study on POD and EST Isozyme of Different Strains of Fusarium oxysporum from Different Crops

利用聚丙烯酰胺凝胶电泳测定了来自不同寄主枯萎病原菌尖孢镰刀菌过氧化物酶同工酶(POD)和酯酶同工酶(EST).结果表明,过氧化物酶同工酶共有3条主酶带,其迁移率分别为:Rf=0.08,Rf=0.20和Rf=0.4.但不同寄主的尖孢镰刀菌POD同工酶存在着明显的差别,来源于花生的尖孢镰刀菌只有2条POD同工酶酶带,其迁移率为:Rf=0.20和Rf=0.08;来源于甜瓜的尖孢镰刀菌只有1条POD同工酶酶带,其迁移率为:Rf=0.20;来源于不同寄主的镰刀菌的EST同工酶谱之间也存在着较大的差别.来源于黄瓜、甜瓜和西瓜的镰刀菌的EST酶谱较为相似,都具有Rf=0.018,Rf=0.114和Rf=1.000的3条酶带,其它寄主来源的镰刀菌的EST酶谱间互相则存在着很大的差别.但这些菌株基本上都存在着Rf为0.018和1.000的2条酯酶同工酶酶带.The peroxidase (POD) and esterase (EST) isozyme of different Fusarium oxysporum from different crops were analyzed by polyarcylamide gel eletrophoresis.The results showed that there were 3 main POD isozme bands;the relative migration rates were 0.08,0.24,and 0.4.The strain from the earthnut had only 2 POD isozyme bands and their relative migration rates were 0.20 and 0.08.The strain from melon had only 1 POD isozyme bands and its relative migration rate was 0.20.There were many differences found in esterase isozyme of the different strains of F.oxysporum,in whixh the main isozyme bands were similar in cucumber,melon and watermelon and their relative migration rates were 0.018,0.114,1.000,and that of the F.oxysporum from the other crops was much different.But they all had the main EST isozme bands whose relative migration rates were 0.018 and 1.000.国家863计划项目(2002AA244031 2);; 福建省计委项目(闽计农经[2002]48号)资