选择8种培养基,采用液体培养法,研究培养基质对尖孢镰刀菌(Fusarium oxysporum Schl.)281菌株小孢子产生量的影响,结果表明,在PDA培养基上培养144h得到最大小孢子产量1.283×109cfu/ml,与其它培养基产孢量差异显著(P=0.05)。培养至192h时,ATCC培养基的小孢子数量达到1.325×109cfu/ml,与PDA培养基之间无显著差异(P=0.05),与其它6种培养基之间差异显著(P=0.05)。综合比较8种培养基,PDA培养基培养时间短,配制简单,是较为理想的产小孢子培养基。The results of eight mediums effect on sporification of Fusarium oxysporum 281 showed that the number of sporification is 1.283×109cfu/ml in PDA medium after 144h,Significant difference were observed between PDA medium and others(P=0.05).The number of sporification is 1.325×109cfu/ml in ATCC medium after 192h,there are no significant difference between ATCC and PDA medium(P=0.05).PDA medium is suitable for sporification.福建省自然科学基金“内生生防菌短芽孢杆菌GFP标记及其对黄瓜枯萎病生防机理的研究”(2006J0068);; 福建省发改委重点项目“生物农药高毒力菌株筛选利用与菌种基因资源库的建立”(闽发改投资[2006]781号
