Design and Implementation of the Online Meal Ordering System

因为网络的发展迅速，人们能在网络中获取实时、更仔细、更有用的信息。在互联网的高速发展的推动下电子商务产生了，人们对网购的喜爱程度越来越深，逐渐成为生活中不可缺少的一部分，更多的人开始喜欢上了网上购物，同时更多的产品出现在网络交易中。开发网上订餐系统的目的就是为了方便广大的消费者生活习惯。 网上订餐是一种新型的餐饮交易方式，对企业而言，是一种新的创业手段，更是对传统餐饮服务的突破。跟传统餐饮业相比较，其优势如下：1方便快捷、消费者不需要到店铺消费；2成本低：不需要占用大量的场地；3管理方便：通过信息化平台进行管理。此网站功能包括前台客户个人操作功能，后台管理员使用功能。因此网站的功能分为前台会...Because of the rapid development of the network, people can get real-time, more detailed and more useful information in the network. Driven by the rapid development of the Internet, e-commerce has been generated, people love the online shopping more and more deeply, and gradually become an indispensable part of life, more people began to like online shopping, and more products appear in the networ...学位：工程硕士院系专业：软件学院_软件工程学号：X201323165

映画に見るアジア系移民 : 北米在住の日中移民像

筑波大学 (University of Tsukuba)201

映画に見るアジア系移民 : 北米在住の日中移民像

筑波大学 (University of Tsukuba)201

Application of proteomics study to red tide algae

国家自然科学基金资助项目(40206018

自组装单层保护金纳米团簇的量子化电容充电

本文研究自组装单层保护金纳米团簇(C12Au MPC)在常温下二氯甲烷溶液中的量子化电容充电效应.示差脉冲伏安曲线显示金核平均直径为2.0 nm的C12Au MPC在-0.6~0.6 V电位区间内有9个明显的量子化电容充电峰,其双电层电容总的变化趋势为在零电荷电位附近最小,随着电位正移或负移电容变大.而且随着该金核尺寸的增大,MPC双电层电容值也变大

Isolation of Total Cellular DNA from Three Species of Lauraceae

为了从富含次生代谢物的樟科植物肉桂、锡兰肉桂、阴香中获得高质量DNA ,研究和改进了CTAB法、高盐低pH法和尿素法。改进方法包括 :1)在裂解液中加入 2 % β -巯基乙醇和 5 %PVP ,以防止氧化褐变的发生 ;2 )在酚 :氯仿抽提前加入 1 5mol L醋酸铵冰浴处理 ,能降低DNA的黏性。所得DNA的质量和产量经电泳、紫外吸收A2 60 A2 80 、PCR扩增和限制性内切酶酶切检测 ,结果表明改进法提取的DNA质量要比常规法的好。其中改进的CTAB法获得的DNA纯度最高 ,能用于PCR扩增和限制性内切酶酶切 ,是提取这 3种樟科植物总DNA的最佳方法。CTAB method,low pH medium with high salt method (LPHS),and urea method were used and improved to extract high-quality DNA from Lauraceae plant Cinnamomum cassia Presl.,C.zeylanicum and C.burmannii,which are rich in secondary metabolites.The modified protocols involved two key steps:1) adding 2% β-mercaptoethnoal and 5% polyvinylpyrrolidone (PVP) in extraction solution to inhibit the oxidization of polyphenols;2) treating with 1.5?mol/L NH 4Ac and incubation at 0℃ prior to supernatant extraction with phenol:chloroform to lower the viscosity of DNA.The yields and qualities of DNA were analyzed by electrophoresis,ratios of A (260)/A (280),PCR amplification and restriction digestion.The results showed that the modified methods are better than the original ones in terms of the quality of total DNA.Among them,the modified CTAB method,which can produce high quality DNA suitable for PCR amplification and restriction digestion,is the best one for DNA extraction from three species of Lauraceae.厦门大学预研基金资

DNA/CdS纳米粒子复合体系的光谱和光电化学性质

在水溶液中以DNA作为模板和稳定剂,构筑了DNA与CdS纳米粒子复合体系(DNA/CdSNPC),研究DNA的含量,单双链等对复合体系光电响应的影响,并综合TEM,UV-Vis,IR和荧光光谱等对其形貌和光谱性质进行表征.结果表明,CdS纳米粒子(CdSNPs)与DNA链之间主要通过静电作用结合;DNA模板对CdSNPs的禁带宽度没有影响;以DNA模板合成的CdSNPs具有较高的表面态密度,其对CdSNPs的荧光有增强作用,而对光电流响应有抑制作用,并且DNA在复合体系中的含量影响荧光增强和光电流减弱的程度.该复合体系在荧光标记检测和DNA的定量分析方面可能具有应用前景

以信用监管为核心 打造新型监管机制

背景材料:近年来,以信用监管为核心的新型监管制度在各个领域被广泛运用。5月15日,《司法部关于印发开展证明事项告知承诺制试点工作方案的通知》确定在13个省(市)和5个国务院部门开展试点,强调转变监管方式,加强信用监管,切实减少繁琐证明。今年3月,国务院办公厅印发《关于全面开展工程建设项目审批制度改

Spectroscopic and photoelectrochemical properties of DNA/CdS nanoparticle composites

DNA/CdS nanoparticle composites (DNA/CdS NPC) containing single or double strands and different concentrations of DNA were constructed in aqueous solutions. The effect of DNA on the photoelectrochemical properties of DNA/CdS NPC was investigated and the spectroscopic properties of DNA/CdS NPC were characterized by TEM, UV-Vis, IR, and fluorescent spectrometry. The results showed that CdS nanoparticles (CdS NPs) were combined with DNA strands through the electrostatic interaction; DNA templates did not affect the band gap of CdS NPs; DNA-templated CdS NPs had a higher density of surface states than that stabilized by Na4P2O7, which enhanced the photoluminescence (PL) intensity whereas restrained the photocurrent response of CdS NPs. Besides, there was certain dependency of both the increase of PL intensity and the decrease of photocurrent response on DNA concentrations in DNA/CdS NPC. The composites were hopeful for applications in both fluorescent tagged detections and quantitative analysis of DNA