亚贡及同属植物化学成分研究进展

亚贡Smallanthus sonchifolius产于南美安第斯地区,是当地印第安人的传统水果。该植物具有抗糖尿病、抗氧化、防衰老、促进肠蠕动、防止便秘等多方面的保健功能。近年来,亚贡在我国成功引种,为了更好地了解并开发这一植物资源,对该属植物的化学成分研究作一综述

亚贡叶的化学成分研究

目的研究新引种的抗糖尿病植物亚贡Smallanthus sonchifolius叶的化学成分。方法应用多种柱色谱方法进行分离和纯化,NMR和MS等波谱解析化合物结构。结果从亚贡叶的乙醇提取物中分离出6个化合物,其结构分别鉴定为:5,8-二羟基-(5H,8H)-β-紫罗兰醇(Ⅰ)、对映-贝壳杉烷-3β,16β,17-三醇(Ⅱ)、对映-贝壳杉烷-16β,17-二醇-19-酸(Ⅲ)、3,4-二羟基苯甲醛(Ⅳ)、正二十五醇(Ⅴ)和正二十八醇(Ⅵ)。结论化合物~均为首次从本植物中分离得到,化合物为首次发现的β-紫罗兰醇型新化合物,命名为亚贡醇(sonchifolol)

Quantitative Determination of Smaditerpenic Acids A and C in Leaves of Smallanthus sonchifolius by HPLC-MS

通信作者：[email protected][中文文摘]建立了同时测定亚贡(Smallanthus sonchifolius)叶中亚贡二萜酸A和C含量的方法.采用高效液相色谱-质谱法,多反应监测(MRM)模式,大黄素为内标,色谱柱:UltimateTM XB-C18(150mm×4.6mm,5μm);流动相:V(甲醇)∶V(0.5%醋酸水溶液)=90∶10;流速:1.0mL/min;柱温:25℃.亚贡二萜酸A和C的线性范围均为0.2～10.0μg/mL(r≥0.999),平均加样回收率(n=6)分别为96.6%和92.4%,相对标准偏差(RSD)分别为1.9%和3.2%.实验数据表明,该方法操作简单、准确,且重复性好,可用于亚贡叶的质量控制.[英文文摘]A new fast and sensitive high-performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS) method was developed and validated for smaditerpenic acids A and C in leaves of Smallanthus sonchifolius using emodin as internal standard(IS).The calibration curves of smaditerpenic acids A and C were investigated with a good linear relationship within the rangs of 0.2-10.0 μg/mL(r≥0.999),and the recoveries(n=6) were 96.6% and 92.4% with RSD of 1.9% and 3.2%,respectively.The analytes were detected in negative ion mode using multiple reaction monitoring(MRM).The method was validated and the specificity,linearity,lower limit of quantitation(LLOQ)precision,accuracy,recoveries and stability were determined.福建省自然科学基金项目(2009J01192);厦门市重大科技计划项目(3502Z20100006