Bone Proteomic Analysis About Chinese Medicine Action on Rat Glucocorticoid-induced Model of Osteonecrsis.

目的:用蛋白质组学的研究方法进行激素性骨坏死的病理和中药作用机理的研究,有利于临床治激素性骨坏死。方法:按照常用激素性骨坏死的造模方法,建立大鼠坏死模型,并设立中药治疗组及空白对照组,经过6周处理后经骨形态学检查,确定骨坏死造模成功,处死动物取股骨和肱骨,提取骨组织蛋白质样品,双向电泳分离,得到各组骨组织总蛋白质分子解剖图谱,用图像分析软件,找到各组间差异蛋白质点,进一步行胶内酶切,M AD ITOF/M S质谱分析,得到各差异点的蛋白质肽指纹图谱,结合蛋白质生物信息库(M atrix sc ience L td database),对各蛋白质进行初步鉴定。结论:初步鉴定了3个差异蛋白,分别为阻凝蛋白重链ⅡB、磷脂谷胱甘肽过氧分酶及泛素化酶E 2(MW:17 kd),初步认为这三种蛋白质在激素性骨坏死的发病及中药治疗过程中发挥着重要调控作用。Objective:To study the mechanism about glucocorticoid-induced osteonecrosis and Chinese Medicine action on osteonecrosis in proteomics,it is beneficial to prevention and cure this disease.Methods:Rat was randomly divided into three groups:cortrol group,osteonecrosis model group and Chinese Medicine therapy group.In the present proteomic study ,we characterized the potential effects of glucocorticoids and Chinese Medicine on protein expression in rat bone.Using two-dimensional gel electrophoresis,mass spectrometry,and Matrix science Ltd database,we elementarily identified three variational proteins.Conclusion:Three proteins were identified as proteins similar to Myosin heavy chain ⅡB,Phospholipid hydroperoxide glutathione peroxidase and ubiquitin-conjugating enzyme E2-17kD.These proteins have been documented to be glucocorticoid-relted proteins.These results can provide valuable experimental evidences for the research of the molecular mechanism of osteonecrosis response to glucocorticoids and Chinese Medicine in bone.获得国家自然科学基金资助(30273631,3010244);; 广东省自然科学基金(020778);; 广东省教育厅基金(Z02011

Biomass pyrolysis technologies based on infrared heating and key parameters of reaction device

生物质是一种清洁的可再生能源,生物质快速热解技术对生物质的利用很重要.本文提出将红外加热应用于生物质热解反应.根据生物质及其热解过程中的大分子中间产物的红外光谱图分析确定生物质热解过程中的红外吸收带,选择红外涂料.设计出基于红外加热的生物质热解反应器的炉体结构,应用Ansys Fluent对炉体燃烧室进行燃烧数值模拟,确定了最佳温度场分布

厚表层Si柔性绝缘衬底上SiC薄膜的外延生长

利用LPCVD方法,在厚表层Si(SOL≈0.5μm)柔性绝缘衬底(SOI)(001)上外延生长出了可与硅衬底上外延晶体质量相比拟的SiC/SOI,表明SOI是一种很有潜力的柔性衬底. Raman 光谱结果表明SiC/SOI外延层比SiC/Si外延层有更大的残存应力,对此从理论上进行了解释.利用X射线衍射(XRD)、原子力显微镜(AFM)、扫描电镜(SEM)和喇曼散射光谱(RAM)技术研究了外延材料的晶体结构、界面性质和应变情况

The Method of Extracting the Protein Specimen from Rat Bone for Further Proteomics Analysis

蛋白质组学是目前研究的重要热点.该文拟建立大鼠骨组织蛋白质样品提取的最佳方法,用于双向电泳分离,为进一步进行蛋白组学分析奠定基础.实验对3种不同方法制备的样本进行同一条件的二维电泳(2- DE)图谱,比较2- DE的结果.结果发现,丙酮沉淀法最佳.这些结果提示,只有结合骨组织成分特征与双向电泳的具体要求,选择恰当的样品提取液,采用合理的提取步骤,获得蛋白质样品,才能得到清晰高分辨率的电泳图谱,满足蛋白质组学分析需要.Proteomics is a challenging field that has been growing rapidly in the postgenomic era.Protein preparation is a key procedure for two-dimensional electrophoresis (2-DE) proteomics.In the current study,three procedures of protein sample preparation,namely basic,dialysis and acetone methods,were compared for establishment of an optimal method to extract proteins from rat bone,based on 2-DE maps from protein samples prepared by the three methods.Of the three methods,acetone method is the best one.Using this method to purify proteins from rat bone,high quality of peptide mass fingerprint can be achieved.The procedure of the method is as follows.Prepared bone (1.0 g) was placed in an earthen bowl to grind into powder in liquid nitrogen and then 3 mL ice-cold buffer (1.44 g urea,0.46 g sulfocarbamide,0.12 g CHAPS,0.047 g DTT,0.015 g Tris-base,30 μL 100 mmol/L PMSF) was added to it.After stored at 4℃ overnight,these samples were centrifuged at 12 000 g for 30 min.The supernatant was dialyzed against 0.01 mol/L PBS for 24 h.Then ten times of volumes of cooled acetone were added to the specimen for the concentration of proteins.Followed by the store at -20℃ for 2 h,the proteins were obtained by centrifugation and then dry at 4℃.Before the isoelecric focusing,the sample was redissolved in the buffer (7 mol/L urea,4% CHAPS,100 mmol/L DTT,40 mmol/L Tris,2 mol/L sulfocarbamide,0.5% ampholyte (pH 5~7),1 mmol/L PMSF) at the room temperature for 2 h.The results may provide a valuable method of sample preparation for study of bone proteomics.国家自然.3010244);; 广东省自然科学基金(020778);; 广东省教育厅基金(Z02011)资

The Application of Proteomics on Modernized Study of TCM

蛋白质组学是后基因组时代的最重要研究课题,能够深化对生命现象本质的了解。对于中医药现代化研究,蛋白质组学作为高通量平行筛选技术在理论体系上与中医药的整体观相契合,能够深化证候的内在本质研究及中药复方的组成与作用机理研究,并能有力促进中药新药开发随着蛋白质组学相关技术的发展完善,其对中医药现代化研究的帮助将进一步扩大。Proleomics is the most important subject of postge-nomic period, which can deepen the understanding of the essence of life. In the modernized study of TCM, the high - flux paralled -screening technique of proteomics in coincident with the wholistic concept of TCM, and can deepen the study of intrinsic quality of TCM syndrome and the composition and mechanism ol herbal formula and strongly promote the development of new herbal medicine. Following with the development and consummation of proteomics and its relevant technique, the modernized study will be beneficial and further developed.国家自然科学基金(编号:30273631) 广东省自然科学基金(编号:020778

日光诱导叶绿素荧光估算中国典型生态系统总初级生产力的能力

陆地生态系统总初级生产力(GPP,Gross Primary Productivity)是陆地生态系统碳循环的重要分量,提高其估算精度具有重要的科学意义。由于受多种因子的影响,GPP的时空变异明显,其估算结果存在较大的不确定性。日光诱导叶绿素荧光(SIF,Sun-Induced Chlorophyll Fluorescence)与GPP密切相关,近年来被应用于估算区域和全球GPP,但其在中国生态系统的适用性尚不清楚。以中国8个典型植被生态系统为研究对象,驱动两叶光能利用率模型(TL-LUE,TwoLeaf Light Use Efficiency Model)模拟以站点为中心0.5°×0.5°区域内的月GPP,验证SIF估算GPP的能力。结果表明,SIF具有监测中国典型植被生态系统GPP的能力,月SIF与TL-LUE模拟的月GPP之间显著相关,其中5个生态系统中两者的R2高于0.8,最高达到0.91,GPP与SIF变化的斜率随生态系统类型变化。模拟的GPP与SIF遥感数据的季节变化特征相同,两者之间的一致性在生长季节好于非生长季节;SIF能更好地监测农田GPP的季节变化