Expression ，Role and Mechanism Studies on pygo2 gene in brain Glioma

Pygo2基因在脑胶质瘤中的表达、作用及机制研究目的：探讨Wnt信号重要成员Pygopus2(Pygo2)在人脑胶质瘤中的表达水平及与脑胶质瘤恶性程度之间的关系,构建pygo2外源基因过表达质粒及RNA干扰质粒。通过建立外源pygo2过表达和pygo2表达抑制的细胞模型，探讨pygo2过表达对胶质瘤细胞的生长以及某些表型特征的影响。通过体内、体外RNA干扰实验来探究pygo2表达下调对脑胶质瘤恶性增殖的影响以及可能的机制，为脑胶质瘤的基因治疗提供基因靶向策略及理论依据。方法：采用免疫组化方法检测Pygo2蛋白在80例脑胶质瘤和5例正常脑组织标本中的表达水平，利用实时荧光定量PCR的方法检测5个...Expression ，Role and Mechanism Studies on pygo2 gene in brain Glioma Purpose:To study the expression level of Pygopus 2(Pygo2)，a new regulation protein of Wnt signaling in human glioma and glioma cell lines, and its relationship with its malignancy grading.to construct exogenous pygo2 gene overexpression plasmid and RNAi plasmid。by transfecton of the plasmid into glioma cell lines,to study the e...学位：博士后院系专业：生命科学学院生物医学科学系_肿瘤学学号：BH1700019

与时俱进的纲领——浅析七大、十五大和十七大的党章修改

党章是党的行动纲领。一个政党的理论水平、发展进步在党章中都会有所反映。中国共产党从诞生至今,与时俱进,对党章进行了十几次的修改,其中党的七大、十五大和十七大对党章的修改具有划时代的重大意义

校园宣讲会的营销传播价值分析

校园宣讲会作为企业常用的一种校园招聘形式,是当前大学生求职的重要渠道,是每年大学校园关注的热点事件之一。本文通过对校园宣讲会的分析,发现在这个过程中受众对企业信息接受存在主动性、企业信息传播互动与深入、受众对企业信息多级传播、可以培养企业潜在目标客户等特点,使得校园宣讲会具有营销传播价值。而如何更大地实现这种价值,基于此,一是要让相关人员真正认知到这种价值的存在,二是在认知基础上围绕品牌形象进行具体的诠释与传播

Classification Analysis Based on Literature about TCM Syndrome Types of Depressive Disorder

在遵循循证医学原则的前提下,系统检索近10年来抑郁症中医及中西医结合临床研究类文献。并将检索的文献按照证候研究、方药治疗、辨证论治、综述进行分类,根据各类文献的研究特点采用不同的统计方法,对抑郁症证候分类进行分析研究,以期总结归纳出抑郁症临床常见证候,为抑郁症中医证候规范化、客观化提供参考依据。统计分析结果显示抑郁症临床常见证候依次为:肝气郁结、肝郁脾虚、心脾两虚、痰气郁结、肝郁肾虚、气郁化火。On the premise of following evidence-based medicine,we searched the clinical research literature of traditional Chinese medicine( TCM) and combined traditional Chinese and western medicine on depressive disorder in nearly a decade comprehensively.We classified these literature according to the syndrome types research,clinical therapy,syndrome differentiation and treatment and literature review.On the basis of the characteristics of literature and different statistical methods,we analyzed the syndrome classification of depressive disorder to summarize the common syndrome types of clinical depressive disorder,providing some references for the standardization and objectification of syndromes for depressive disorder.Statistical analysis results show that the depressive disorder common clinical syndromes in the order:stagnation of liver qi,stagnation of liver qi and spleen deficiency,heart-spleen deficiency,phlegm-qi stagnation,liver depression and kidney deficiency,qi depression transforming into fire.福建省自然科学基金项目(2014J01374);; 厦门市科技计划项目(3502Z20133007

LAS在滇池典型入湖河口缺氧水体中的生物降解

利用"河水衰减法"研究了不同培养条件下阴离子表面活性剂LAS在滇池典型入湖河口(海河口)缺氧水体中的生物降解及其动力学,探讨了温度、pH值、LAS的初始浓度、添加营养盐(NH4Cl或NaH2PO4)以及曝气等因素对LAS生物降解的影响.结果表明,在河口水体中的不同条件下,试验26 d后LAS的生物降解率都达95%以上,且降解服从二级动力学模型.温度、LAS的初始浓度、添加营养盐以及曝气均对LAS的降解有一定的影响.当水温从10℃增至25℃时,其降解速率p从0.21 d-1增至0.90 d-1;在连续曝气

从舌辨治月经后期临床体会

月经后期为妇科常见病证,临床常因无明显症状或症状多而杂,给辨证带来一定难度。舌诊作为中医诊病的特色诊法,辨治月经后期具有重要作用。文章通过医案2则,探讨舌诊在诊治月经后期中的临床意义,以期更好地指导临床诊疗。国家自然科学基金项目(No.81673660)~

Construction of cDNA library of gills from Pagrus major with the cloning of hepcidin

【中文摘要】 以细菌攻毒的真鲷鳃组织为材料,分离出mRNA,合成双链后,回收500~4 000bp cDNA片段,构建了λZAP表达型cDNA文库。初级文库的容量为1.75×105个重组子,扩增文库的滴度达到1×109pfu.mL-1,随机挑选噬菌斑的插入片断在500~2000bp之间。以hepcidin特异性引物做PCR扩增文库,获得了hepcidin抗菌肽基因cDNA序列,证明所建立的文库可用于免疫相关基因的筛选。本文库可作为筛选真鲷免疫相关基因的重要资源。 【英文摘要】 With the layers of mucus and nonspecific immune defenses,the fish gill is exposed to the external environment and forms the initial barrier to invasion by pathogens.As one of the mucosa-associated lymphoid tissues in teleost fishes,gill contains populations of leucocytes.In order to probe the immune-related genes in red seabream(Pagrus major),the cDNA library of gills was constructed from the red seabream inoculated with several species of pathogenic bacteria.The total RNA was extracted by TRIZOL and mRNA w...福建省重大科技项目(2003I005);; 厦门市高新技术项目(3502Z20021052

基于一维TiO2纳米管阵列薄膜的β伏特效应研究

介绍了一种采用宽禁带半导体二氧化钛纳米管阵列薄膜材料制备β伏特效应同位素电池的方法.通过对金属钛片的电化学阳极氧化制备了垂直定向、有序排列的二氧化钛纳米管阵列薄膜,研究了退火条件对二氧化钛纳米管阵列薄膜半导体光电性能的影响.通过与镍-63辐射源的集成封装,形成三明治结构镍-63/二氧化钛纳米管阵列薄膜/钛片的β伏特同位素电池.实验结果表明,基于氩气氛围下450?C退火的黑色二氧化钛纳米管阵列薄膜具有高的氧空位缺陷浓度和宽的可见-紫外吸收光谱.在使用β辐射总能量为10 m Ci的镍-63辐射源时,同位素电池的开路电压为1.02 V,短路电流75.52 n A,最大有效转换效率为22.48%.国家自然科学基金(批准号:61574117);;深圳市科技计划项目(批准号:JCYJ20170306141006600)资助的课题~

LED Lighting Design of Underground Parking Lots Based on DILAUX

目前由于汽车的广泛普及,对城市的地下车库照明提出了更多的要求,但随着新一代照明光源lEd照明技术进一步的发展与完善,使得地下车库照明有了更节能、更环保、更经济的可能。在dIlAuX中建模57x33.3M2的中型地下车库,并利用dIlAuX计算软件对地下车库的lEd照明进行模拟、计算、优化,不断的调整灯具位置以及灯具间隔来到达最佳的照明效果,通过实景分析来探讨lEd在地下车库照明领域应用的可行性及其应用前景,并为地下车库照明提供一种设计上的借鉴。More requirements for the light system of civil underground parking lot lighting are concerned since cars are wildly-used nowadays.And it becomes possible to make the light system of underground parking lot more energy-saving,more environmental friendly and more economic as the new generation of LED technology gets more and more developed and improved.DILAUX is a kind of lighting calculation software,and it is used to model an underground parking lot with a medium 57 × 33.3m2and some simulations,calculations,and optimization on the LED light of the parking lot are also done by DILAUX.Therefore,constantly adjust locations and intervals of the lighting fixtures to achieve the optimal results.Furthermore,the feasibilities and application prospects of LED lighting applications in the underground parking lot field can also be explored through the real analysis.Overall,it shows an example to design the light system for civil underground parking lots.国家自然科学基金(61301009); 福建省重大科技项目资助(2006H0092); 福建省自然科学基金计划资助项目(2013J01252

Construction of eukaryotic vector pcDNA3.1-flag-pygo2 and expression in C6 cell

目的构建pcDNA3.1-flag-pygo2真核表达载体并在C6细胞中进行表达。方法从小鼠脑胶质细胞中提取总RNA,RT-PCR法反转录合成c DNA,设计引物,调取目的片段,与pcDNA3.1-flag载体连接后转化大肠杆菌DH5α,LB平板筛选菌落,提取质粒。重组质粒pcDNA 3.1-flag-pygo2经过酶切鉴定及测序后,阳离子脂质体法转染C6细胞并经免疫细胞化学染色及蛋白印迹检测重组体的表达。结果重构质粒pcDNA 3.1-flag-pygo2经限制性核酸内切酶EcoRⅠ,HindⅢ酶切分析及测序检查,表明真核表达载体构建正确;瞬时转染C6细胞后,免疫细胞荧光染色及蛋白印迹检测表明转染细胞能够表达外源Pygo2基因。结论成功构建了pcDNA3.1-flag-pygo2真核表达载体并能够在真核细胞中进行表达,这为今后研究pygo2基因在胶质瘤中的作用机制奠定了基础。Objective To construct the eukaryotic expression vector pcDNA3.1-flag-pygo2 and express the combined protein in C6 cell line.Methods To extract total RNA from primary glial cell of mouse and to synthesize c DNA by RT-PCR,then design primer and clone whole segment of pygo2 gene.After the targeted gene was inserted into vector pcDNA3.1-flag,the recombined plamid was transformed into E coli DH5α for LB agar plate screening and the recombined plasmid were extracted and purified.After verification by double enzyme digestion and sequencing.,the constructed eukaryotic expression plamid was transfected to C6 cell line by lipofectamin method,finally the protein expression was detected by immunocytochemical staining as well as western blot.Results The new constructed vector was confirmed by restricted enzyme Eco R I,HindIII digestion assay and correct Pygo2 was verified by sequenceing.finally,pcDNA3.1-flag-hpygo2 can express exogenous pygo2 gene in glioma C6 cell line after transient transfection by the determination of immunocytofluorescent staining and western blot.Conclusion The new plamid pcDNA3.1-flag-pygo2 was constructed successfully,and can express fused protein in eukaryotic cell,which establish the foundation for future research on pygo2 gene function in human glioma