Status，Problems and Countermeasure of X Airport Terminal Business Franchise

中国经济经过几十年的发展，民航业在国民经济中占有的地位越来越重要，传统意义上机场的航空服务主业就是为飞机起降、停泊、旅客候机提供服务，非航空服务业务并不受重视。但是，从国际上先进大机场的经验来看，非航空服务业务的发展在机场的主营业务中不可或缺，也能带来更高的收益。候机楼商业是非航空服务业务的重要组成部分，它的发展不仅可以确保民航业尽快收回初始投资成本并实现盈利，也能为旅客、商业投资者、城市居民及内部服务后勤人员，创造和谐的旅行、生活、工作及娱乐的共享空间。 国内研究候机楼商业特许经营的学术论文较少，一般旅客对候机楼商业消费的体验评价也不佳，这似乎是个偏僻、被遗忘的荒芜角落。本文力图通过对候机...After decades of development of the Chinese economy, civil aviation plays a more and more important role in the national economy. Traditionally, the main industry of the airport includes taking off and landing of the aircraft, parking, and passenger terminal services, non-aeronautical services and business are neglected. However, from the advanced experience of international airports, the developm...学位：工商管理硕士院系专业：管理学院工商管理教育中心（MBA中心）_工商管理硕士(MBA)学号：X200715513

荧光分光光度法测定天然水中微量氟

本文叙述新建立的氟的Al-荧光镓试剂络合物荧光熄灭测定法,该法激发波长365nm,测试波长575nm。pH3.0和pH4.0时其线性范围分别为5-80ppbF-和5-300ppbF~-。40ppbF~-时标准偏差为2ppbF~-,几种水样经和氟化镧电位法,茜素络合剂分光法进行对照测定,结果一致

湖北贫困县规划统筹策略及脱贫路径

我国扶贫开发已进入\"啃硬骨头\"、攻城拔寨的冲刺期,而中西部地区是我国扶贫攻坚的重点地区。文章运用自组织理论,分析了湖北25个国家级贫困县的人口、产业和空间的城镇化\"自极化\"特征,揭示了外部作用薄弱—边界作用抑制—内部作用极化的形成机制,从空间、等级和职能三个方面提出了城镇体系的规划统筹策略,并结合湖北贫困县的实践经验提出\"两个转移,一个对接\"的脱贫路径。国家自然科学基金项目(51608404、51708233、51478199、51538004、51708471);;中央高校基本科研项目(2016IVA031、2017IVB044

An Investigation of the Professional Development of Primary and Secondary School P. E. Backbone Teachers in Fujian Province

采用文献法、调查法、统计法、逻辑法等研究方法,以参加2013—2015年福建省基础教育万名骨干教师培训中的190名中小学体育骨干教师为调查对象,对教师基本情况和教师专业发展的认识、态度、知识、能力、继续教育等现状进行调查,在此基础上对教师专业发展现状进行分析,总结出我省中小学体育骨干教师专业发展的特点及存在的问题,并提出相应的建议,给我省中小学体育教师培训机构的改革提供依据,同时为我省中小学体育教师专业发展提供参考与借鉴,为进一步深化我省中小学体育教育改革提供人才保障.结果表明:1)目前,福建省中小学体育骨干教师以年富力强者居多,年龄、职称结构较合理;教师专业发展意识强,学历层次由大专逐步向本科、硕士过度,但硕士比例小;2)教师专业发展态度端正,职业的稳定性和责任感强烈,但部分年轻教师缺乏专业发展的自主性;3)教师对专业知识的掌握比较扎实,主要通过互联网、图书资料、专家报告或讲座等方法来获取专业知识;4)专业能力水平方面,教师在体育教学、课外指导、体育保健、现代教育技术运用等方面能力比较强,而创新教育和科研两方面能力相对比较薄弱;5)当下,我省中小学体育骨干教师最需要强化的培训是课题申报技能、论文写作能力、专题讲座能力、教育科研方法、提高学历层次、教学设计能力,其中提高科研能力和水平是教师们最迫切的愿望.2014年福建省中青年教师教育科研项目(社科A类)(JAS14023

EXPERIMENTAL STUDY ON FRACTAL DIMENSION CHARACTERISTICS of ACOUSTIC EMISSION of ROCK UNDER MULTILEVEL UNIAXIAL CYCLIC LOADING

通过对三种岩石试样进行单轴循环加载试验,获得岩石试样加载过程中声发射事件率、能量率和空间位置分布数据。运用相空间重构理论直接从时间序列上通过g-P算法求得事件率和能量率关联分维,根据柱覆盖法求解得到声发射事件源空间分布关联分维。研究结果表明,声发射事件率、能量率和空间分布都具有分形特性,且声发射源空间分布分形特性最为显著。相空间的选取对关联维数有一定影响,相空间取值为4时能够较好地计算关联维数。将不同加卸载循环声发射源空间分布的变化趋势和声发射源空间分布关联维数变化趋势进行对比发现,声发射关联维数能够很好地反映岩石内部损伤破坏的发展。在等压加卸载条件下,声发射源空间分布关联维数显著突增。随着加卸载循环应力的增加,关联维数总体呈下降趋势,在达到较高应力时,关联维数则在较小区间内波动或下降趋势变得极其缓慢。该特点可以作为矿山地压微震监测预警的参考依据。Experiments on acoustic emission(AE) characteristics of fractal were carried out under uniaxial multilevel cyclic loading.AE rate, energy rate and spatial distribution of different rock specimens were obtained.Relevant fractal dimensions of AE rate and energy rate were figured out through G-P algorithm based on the theory of reconstruction of phase space.Relevant fractal dimensions of AE spatial distribution was figured out through column-covering method.The results show that AE rate, energy rate and AE spatial distribution are of fractal characteristics,and among them, AE spatial distribution has the most obvious fractal characteristics.Phase space has some effects on relevant fractal dimensions, and it is better to choose 4 as the basic dimension for correlation dimension calculation.The comparison of development trend of AE spatial distribution and relevant fractal dimensions under different cycles shows that fractal characteristics can reflect the statistical evolution of the micro-cracks in rock.Relevant fractal dimension increases under isobaric cyclic loading and decreases with the increasing of stress.Relevant fractal dimensions fluctuate in a small range or decrease slowly when the higher stress has been reached.The characteristics above can be used as the reference for micro-seismic monitoring warning on ground pressure in mining.国家“十二五”科技支撑计划项目(2013BAB02B06); 国家科技部科研院所技术开发研究专项资金项目(2013EG215024

The Multi-criteria Evaluation of Rockburst Proneness on Deep Buried Large Tunnel

研究目的:高铁北武夷山隧道是一座单洞双线隧道,隧道最大埋深达1 100 M,属于高应力区,开挖过程中可能会出现岩爆现象。通过对隧道高应力地区采集的岩样进行室内岩爆倾向性实验,并结合声发射监测技术,得到岩石单轴抗压强度等性能参数,先采用强度脆性系数法、变形脆性系数法、弹性应变能指标法、切向应力准则四种单指标评价方法进行岩爆倾向性评价,然后运用四指标模糊数学综合评价法进行评价。研究结论:(1)结合声发射监测技术进行岩石力学实验,获得的变形脆性系数、弹性应变能指标系数相比常规实验更加精确;(2)根据岩体性质和围岩的应力情况,在传统综合评价方法基础上增加了变形脆性系数法评判指标,结果表明采用模糊数学综合评价方法得到的评价结果与单指标评价相比,更加符合隧道岩爆的实际情况;(3)本研究成果为隧道岩爆预测提供了可行方法,对于类似工程提前防治岩爆有借鉴意义。Research purposes: North Wuyi Mountain of high-speed railway is a single hole bidirectional tunnel,and the maximum depth of the tunnel is as deep as 1100 meters,so it belongs to high stress area that may cause rockburst in the process of excavation.The indoor experiments about rockburst proneness of rock samples which were collected in high stress area of the tunnel are recorded in this paper,and some property parameters of rock like the uniaxial compressive strength were got combining acoustic emission testing.Four kinds of single index evaluation method were used to evaluate rockburst proneness firstly,including the intensity of brittleness coefficient method,the deformation brittleness coefficient method, the elastic strain energy method and the tangential stress criterion.Then the comprehensive evaluation method of fuzzy mathematical was used to evaluate.Research conclusions:( 1) Combined with acoustic emission monitoring technology,we can get two indexes more accurately than conventional experiment.( 2) According to rock properties and surrounding rock stress,we increase the evaluation indicator of deformation brittleness coefficient method.It showed that the results from the comprehensive evaluation method of fuzzy mathematical are more in line with the actual situation of the rock burst of tunnel compared with the single index evaluation.( 3) The research result provides a feasible method to predict rockburst and it is significant to prevent and control of rockburst in advance for similar engineering

Design and Fabrication of Al_2O_3/SiO_2 Double-Layer Antireflection Coatings on 4H-SiC Substrate

在4H-SiC基底上设计并制备了Al2O3/SiO2紫外双层减反射膜,通过扫描电镜(SEM)和实测反射率谱来验证理论设计的正确性。利用编程计算得到Al2O3和SiO2的最优物理膜厚分别为42.0nm和96.1nm以及参考波长λ=280nm处最小反射率为0.09%。由误差分析可知,实际镀膜时保持双层膜厚度之和与理论值一致有利于降低膜系反射率。实验中应当准确控制SiO2折射率并使Al2O3折射率接近1.715。用电子束蒸发法在4H-SiC基底上淀积Al2O3/SiO2双层膜,厚度分别为42nm和96nm。SEM截面图表明淀积的薄膜和基底间具有较强的附着力。实测反射率极小值为0.33%,对应λ=276nm,与理论结果吻合较好。与传统SiO2单层膜相比,Al2O3/SiO2双层膜具有反射率小,波长选择性好等优点,从而论证了其在4H-SiC基紫外光电器件减反射膜上具有较好的应用前景。Al2O3/SiO2 double-layer UV antireflection coatings were designed and fabricated on 4H-SiC substrate,and the validity of theoretical design was further verified by scanning electron microscope (SEM) and reflection spectrum. The optimal physical thickness of Al2O3 and SiO2 is 42.0 nm and 96.1 nm respectively by programming calculation. And then the minimum reflectance of 0.09% is obtained at reference wavelength λ=280 nm. According to error analysis,keeping the sum of double-layer thickness consistent with theoretical value is helpful to reduce the reflectance. In addition,the refractive index of SiO2 should more accurate and the refractive index of Al2O3 should be controlled close to 1.715 in the experiment. Al2O3/SiO2 double-layer coatings were deposited on 4H-SiC substrate by electron beam evaporation and the physical thickness is 42 nm and 96 nm respectively. SEM images show that the deposited layers and the substrate perform good adhesion to each other. The practical minimum reflectance is 0.33% at λ=276 nm which is close to theoretical value. Compared with conventional SiO2 single layer,Al2O3/SiO2 double-layer coatings show low reflectance and better wavelength selectivity. These results make the possibility for 4H-SiC based UV optoelectronic devices with Al2O3/SiO2 films as antireflection coatings

基于矩张量分析的特大山体破坏前兆孕震机制研究

工程岩体震源机制研究是岩体破坏灾害监测预警研究和应用的基础。针对一次特大爆破诱发的采空区上覆岩体产生特大破坏案例的前兆微震定位数据,采用矩张量理论对前兆微震源定位事件进行震源机制解反演,计算监测所得微震定位事件的矩张量并进行分解,获得纯双力偶成分MDC分量;采用Feigner和Young矩张量破裂判据计算得到破坏类型判别参数R值,对前兆微震事件的岩体破裂类型进行判断,同时根据矩张量分量计算震源体积不变部分参数T和体积变化部分参数k,据此绘制并研究了哈德森震源类型-T k图,分析表明前兆微震源的破裂类型主要为剪切破坏;进一步根据矩张量分解所得纯双力偶成分MDC分量,解得岩体震源处的断层参数。将震源机制解分析得到的前兆微震事件剪切破裂类型与现场山体宏观剪滑破坏相对比,其结果是基本一致的。研究表明基于微震矩张量理论对前兆震源机制解的分析,可较为准确地判断中尺度工程岩体破裂类型,该研究可作为进一步的中尺度工程岩体破裂机制研究以及岩体工程灾害预警研究参考。国家自然科学基金资助项目(51674218);;\n国家重点研发计划项目(2016YFC0600702

Promotion of proliferation of luminal B breast cancer cells by mesenchymal stem cells and its underlying molecular mechanisms

目的分析人脐带间充质干细胞(hUC-MSCs)对Luminal B型乳腺癌细胞生长增殖的影响,并初步探讨其可能的分子机理。方法绿色荧光蛋白(GFP)和荧光素酶共表达慢病毒感染人Luminal B型乳腺癌细胞BT474,并经嘌呤霉素筛选两周后,于荧光显微镜下观察GFP的表达情况,IVIS Kinetic成像系统拍照以观察和记录慢病毒感染后BT474细胞荧光素酶的表达情况;荧光显微镜下直接观察,结合MTS实验分析hUC-MSCs共培养或其浓缩上清处理对GFP和荧光素酶共表达BT474细胞生长增殖的影响;Western blot法检测hUC-MSCs浓缩上清处理对BT474细胞Akt和MAPK信号通路激活情况以及下游细胞周期调控蛋白Cyclin D1表达的影响;常规RT-PCR法检测hUC-MSCs中NRG-1、NRG-2、IGF-Ⅰ、IGF-Ⅱ和EGF等配体的表达。荧光素酶表达强度与细胞数量的相关性经由Excel软件行统计学分析,MTS实验数据则经由SPSS13.0统计软件行统计学分析。结果荧光显微镜和IVIS Kinetic成像系统的观察结果分别证实,GFP和荧光素酶经慢病毒载体系统的介导可在BT474细胞中成功地共表达,且荧光素酶的表达强度与细胞数量呈直线相关。MSCs共培养或其浓缩上清处理均可显著促进Luminal B型乳腺癌细胞BT474的生长增殖,其细胞存活比例分别为各自对照组的148.06%(P<0.005)和147.99%(P<0.001);MSCs浓缩上清处理同时激活BT474细胞内Akt和MAPK信号通路,并上调细胞周期调控蛋白Cyclin D1表达。此外,RT-PCR结果显示,hUC-MSCs中NRG-1和EGF的mRNAs水平呈高表达,而NRG-2、IGF-Ⅰ和IGF-Ⅱ等配体的mRNAs表达也可见。结论 MSCs可通过表达并分泌NRG-1等配体,从而激活Luminal B型乳腺癌细胞BT474的下游Akt和MAPK信号转导通路以上调细胞周期调控蛋白Cyclin D1的表达,进而促进其生长增殖。Objective To investigate the effect of human umbilical cord mesenchymal stem cells(hUC-MSCs) on proliferation of luminal B breast cancer cells and its underlying molecular mechanisms. Methods Human luminal B breast cancer cells BT474 were infected with GFP and luciferase co-expressing lentiviruses and then subjected for selection with Puromycin for 2 weeks. The expression of GFP and luciferase was detected by fluorescent microscopy and IVIS Kinetic image system, respectively. The effect of coculture or treatment with conditioned medium of hUCMSCs on proliferation of BT474 was analyzed with MTS assay. Western blot was carried out to detect the effect of treatment with conditioned medium of hUC-MSCs on the activation of both Akt and MAPK signalings in BT474, as well as the expression of downstream cell cycle regulator Cyclin D1. Regular RT-PCR was applied to analyze the mRNAs expression of ligands such as NRG-1, NRG-2, IGF-Ⅰ, IGF-Ⅱ, and EGF in hUC-MSCs. The correlation between relative luciferase activity and cell number was analyzed with Excel software, while MTS assay data was statistically analyzed with SPSS 13.0 software. Results The co-expression of GFP and luciferase in BT474 via lentiviral expression system was visualized by fluorescent microscopy and IVIS Kinetic image system. The linear correlation between relative luciferase activity and cell number was determined by curve fitting analysis. Coculture or treatment with conditioned medium of hUC-MSC significantly promoted the proliferation of BT474, with survival rates being 148.06 %(P < 0.005)and 147.99 %(P < 0.001)of control, respectively. In addition, treatment with conditioned medium of hUC-MSC was shown to induce activation of both Akt and MAPK signalings, which further upregulated the expression of Cyclin D1. Moreover, high mRNAs expression levels of both NRG-1 and EGF, as well as moderate mRNAs expression levels NRG-2, IGF-Ⅰ, and IGF-Ⅱ were showed by RT-PCR. Conclusion Our results here demonstrated that MSCs may promote the proliferation of luminal B breast cancer cells through paracrine of ligands such as NRG-1, which in turn results in the activation of both Akt and MAPK signalings and upregulation of the expression of Cyclin D1.国家自然科学基金面上项目(81272922);; 福建省自然科学基金面上项目(2016J01577);; 福州总医院院内课题国际合作研究专项(2015G01

间充质细胞外泌体促进小鼠胰岛内皮细胞血管生成的研究

目的探讨间充质细胞（MSC）外泌体对低氧条件下胰岛内皮细胞（MS-1）血管生成的影响。方法 MSC无血清低氧条件培养48 h,超滤离心法富集条件培养基中的外泌体,采用电镜和Western Blot的方法进行鉴定;通过血管形成试验比较分析不同条件下:常氧培养组（NOR组,21%O2、5%CO2）、低浓度氧培养组（HYP组,2%O2、5%CO2）、外泌体+低浓度氧共培养组（HYP+EXO组,2%O2、5%CO2）,MS-1细胞的血管形成能力;image J软件分析血管形成长度;PCR、Q-PCR检测血管内皮生长因子（VEGF） RNA水平的表达,Western Blot检测VEGF、HIF1α蛋白水平表达以及mTOR信号通路激活情况。采用单因素方差分析和SNK-q检验统计学分析。结果超滤离心法富集的MSC条件培养基中的外泌体,大小为30～100 nm,表达CD9,CD63,CD81等外泌体表面标志物;血管形成试验结果显示,低氧促进MS-1血管生成,HYP+EXO组形成明显的血管网状结构;HYP+EXO组血管形成相对长度（2386.0±137.7）像素与NOR组（393.3±174.2）像素和HYP组（1467.0±230.0）像素相比增强,差异有统计学意义（t=12.30,P=0.0065;t=15.74,P=0.0040）; PCR结果显示,HYP+EXO组VEGF相对表达量（20.26±9.972）较常氧对照组（1.000）和低氧组（6.521±3.501）均增强,差异有统计学意义（t=5.462,P=0.0009;t=4.238,P=0.0038）;同时,Western Blot结果显示VEGF蛋白水平表达升高,HIF1-α表达上调,mTOR发生磷酸化。结论 MSC外泌体可促进低氧条件下的小鼠胰岛内皮细胞血管生成。MSC外泌体可能通过上调HIF1-α,调节VEGF表达,激活mTOR信号通路,促进胰岛内皮细胞血管生成。国家自然科学基金青年项目(81601618);;福建省自然科学基金面上项目(2016J01582、2016J01580、2018J01349);;福建省科技创新联合资金重大项目(2017Y9127