談話分析からみた日本語学習者と母語話者の聞き手言語行動の実証的研究

首都大学東京, 2016-09-30, 博士 (日本語教育学)首都大学東

基于全球价值链的技术引进与自主创新

与发达国家技术差距较大以及技术日趋复杂化的背景，决定了我国需要以各种模式引进技术，但技术发展战略必须从技术引进模仿型向技术创新自主开发型转变。不同的技术引进模式，可能带来不同的产业技术自主性与自主品牌发展方向。对我国汽车和高铁行业技术引进发展比较分析表明，基于全球价值链的技术引进有助于培育具有较强竞争力的价值链“链主”，并形成本土企业主导的较为完备的全球价值链，进而提升整个价值链的自主创新能力和国际竞争力。国家社科基金重大项目《中国产业创新发展战略研究》(编号:15ZDC013)的阶段性成果

贝叶斯联合模型与中国洞穴鱼类分化时间的估算(鲤科:金线鲃属)

金线鲃属分布于中国云贵高原及周边地区,包括10多种适应于洞穴生活、具有不同程度眼睛和色素退化的典型洞穴鱼类.迄今为止,金线鲃属的系统发育关系还存在很大争议.我们获得了34种金线鲃属鱼类及外类群的细胞色素b和ND4基因序列,用贝叶斯联合模型分析数据,重建了金线鲃属的系统发育关系;并用松散分子钟估算了本属及内部分支的起源时间.金线鲃属的单系性得到强烈的支持,本属可分为6个分支.此外,本研究结果还支持洞穴物种的多系起源假说以及季氏金线鲃(Sinocyclocheilus jii)的基部位置.松散分子钟估算表明

一种四倍体鲤科鱼Sox基因的克隆和序列分析

为了研究重要功能基因在多倍体基因组中的演化情况,通过分子克隆的方法从四倍体大鳞结鱼(Tordouronensis)中获得13条Sox基因序列,分别为SoxB,SoxC和SoxE组成员.对大鳞结鱼Sox基因系统进化分析的结果支持鱼类特异的基因组重复的假说,同时表明,基因Sox19可能为真骨鱼特有的Sox基因家族成员.序列分析显示,与其他物种相比大鳞结鱼中Sox基因的碱基替代,绝大多数为同义替代.结果表明,所得到的Sox基因序列均受到净化选择,同时由多倍化所造成的重复基因对Sox4a,Sox9a和Sox9b

企业技术创新模式选择:自主学习还是合作

国外技术的引进模仿为中国产业快速发展打下了坚实的基础,但也使中国企业与产业的技术发展失去了自主性,造成产业低端化。而且随着中国企业与世界先进国家的技术差距缩小,引进技术的难度与成本增大,因此,中国产业发展需要从引进模仿型向创新自主开发型的技术发展战略转变。本文在构建数理模型分析本土企业是选择自主学习还是与外资企业合作的机理基础上,进一步以中国汽车与高铁行业为例,分析了本土企业从引进模仿到自主研发的战略转变,进而提出相应对策。国家社会科学基金重大项目“中国产业创新发展战略研究”(项目编号:15ZDC013);;国家自然科学基金项目“并购与创新:基于中国装备制造业理论与实证研究”(项目编号:71573219);;广东省自然科学基金项目“广东省装备制造业并购模式选择及其创新绩效”(项目编号:2014A030313007

The spatial and temporal distribution of virioplankton and heterotrophic bacteria in the coastal waters of Zhejiang

为了解浙江近海海域浮游病毒和异养细菌的时空生态分布,于2014年11月(秋)、2015年1月(冬)、2015年5月(春)和2015年7月(夏)连; 续4个季节采集了浙江近海海域表层海水样品,采用流式细胞仪技术对样品浮游病毒和异养细菌丰度进行了检测,对其时空分布特征及与环境因子的相关性做了分析; 。从水平分布来看,在4个季节中浮游病毒、异养细菌丰度均为宁波、沈家门、岱山等沿岸海域站位的丰度低,远陆海域东极和枸杞站位的丰度高。从季节变化来看; ,浮游病毒、异养细菌丰度的季节分布特征同为夏>春>秋>冬,相关性分析结果表明,春、夏、秋、冬4个季节,浮游病毒丰度与异养细菌丰度均为显著正相关。; 浮游病毒丰度在春、秋、冬季节均与病毒/细菌比值(VBR)显著正相关;夏、秋季节均与盐度显著正相关;春、夏季节均与总磷显著负相关;春季分别于与溶解; 氧、pH、化学耗氧量(COD)显著正相关。异养细菌在春、秋、冬季节均与VBR显著正相关;春、夏季节与溶解氧显著正相关,冬季与溶解氧显著负相关;春; 、夏季节与总磷显著负相关;秋、冬季节均与温度、盐度显著正相关;春、冬季节均与COD显著正相关。In order to investigate the spatial and temporal distribution of; virioplankton and heterotrophic bacteria in the coastal waters of; Zhejiang,the representative samples of sea water were collected in the; coastal waters of Zhejiang for 4 consecutive seasons in November; 2014(Autumn),January 2015(winter),May 2015(spring) and July; 2015(summer). The distribution of virioplankton and heterotrophic; bacteria was studied by flow cytometry,and the correlations between the; abundances of virioplankton,heterotrophic bacteria and environmental; factors were also analyzed. From the horizontal distribution,in the four; seasons low abundance of virioplankton and heterotrophic bacteria was in; the stations of Ningbo,Shenjiamen,Daishan immediate offshore; area;high-abundance was in the stations of Dongji and Gouqi offshore; area. From the seasonal distribution,the abundance of the virioplankton; and heterotrophic bacteria was the same as that of; summer>spring>autumn>winter. The abundance of virioplankton and; heterotrophic bacteria was significantly and positively correlated in; the 4 seasons. The abundance of virioplankton was significantly and; positively correlated with VBR in spring,autumn and winter. The; abundance of virioplankton was significantly and positively correlated; with salinity in summer and autumn. The abundance of virioplankton was; significantly and negatively correlated with TP in spring and summer.; The abundance of virioplankton was significantly and positively; correlated with dissolved oxygen,pH,COD in spring. The heterotrophic; bacteria were significantly and positively correlated with VBR in; spring,autumn and winter. The heterotrophic bacteria were significantly; and positively correlated with dissolved oxygen in spring and summer but; were significantly and negatively correlated with dissolved oxygen in; winter. The heterotrophic bacteria were significantly and negatively; correlated with TP in spring and summer. The heterotrophic bacteria were; significantly and positively correlated with temperature and salinity in; autumn and winter. The heterotrophic bacteria were significantly and; positively correlated with COD in spring and winter.国家海洋局公益性行业科研专

Spatial and Temporal Distribution of Picophytoplankton in Coastal Waters of Zhejiang

【目的】为了解浙江近海海域超微型光合自养浮游生物原绿球藻(Prochlorococcus,; Pro)、聚球藻(Synechococcus,Syn)和超微型真核浮游植物(Picoeukaryotes,Euk)的时空生态分布,【方法】于20; 14年11月(秋)、2015年1月(冬)、2015年5月(春)和2015年7月(夏)连续4个季节采集了浙江近海海域表层海水样品,采用流式细胞仪技; 术对样品超微型光合自养浮游生物丰度进行了检测,并对其时空分布特征及与环境因子的相关性进行了研究。【结果】从水平分布来看,在4个季节中超微型光合自; 养浮游生物丰度均为宁波、沈家门、岱山等沿岸海域站位的丰度低,远陆海域东极和枸杞站位的丰度高。从季节分布来看,原绿球藻的季节分布特征为夏季>秋季; >冬季>春季,聚球藻的季节分布特征为秋季 >冬季 >春季>夏季,超微型真核浮游植物的季节分布特征为夏季>秋季; >春季>冬季。相关性分析表明,春季,原绿球藻、聚球藻、超微型真核浮游植物均与pH显著正相关;秋季,原绿球藻、聚球藻、超微型真核浮游植物均与温度显; 著正相关;冬季,聚球藻、超微型真核浮游植物均与盐度显著正相关。【结论】本文超微型光合自养浮游生物丰度近岸海域明显低于远陆海域,这与近岸海域泥沙含; 量大,水色和透明度低有很大关系。浮游病毒对宿主的裂解也会影响超微型光合自养浮游生物的丰度变化。【Objective】 The preseat paper aims to investigate the spatial and; temporal distribution of picophytoplankton: Prochlorococcus,; Syn-echococcus and Picoeukaryotes in the coastal waters of; Zhejiang.【Method】 The representative samples of sea water in the coastal; waters of Zhejiang were collected for 4 consecutive seasons in November; 2014 (Autumn), January 2015 (winter),May 2015 (spring) and July 2015; (summer). The distributions of picophytoplankton by flow cytometry were; analyzed, and the correlation between the abundances of; picophytoplankton and environmental factors were studied.【Result】 From; the horizontal distribution, in the four seasons, the abundance of; picophytoplankton were low-abundance areas in the stations of; Ningbo,Shenjiamen, Daishan immediate offshore area, high-abundance areas; in the stations of Dongji and Gouqi offshore area. From seasonal; distribution, the abundance of Prochlorococcus was in order of summer >; autumn > winter > spring, the abundance of Synechococcus autumn > winter; > spring > summer; and the abundance of Picoeukaryotes summer > autumn >; spring > winter. Prochlorococcus,Synechococcus and Picoeukaryotes were; significantly positively correlated with pH in the spring.; Prochlorococcus,Synechococcus and Picoeukaryotes were significantly; positively correlated with temperature in autumn. Synechococcus and; Picoeukaryotes were positively correlated with salinity in; winter.【Conclusion】In this study, the abundance of picophytoplankton in; immediate offshore area was significantly lower than that of far; offshore area. The reason was that the sediment content was large,the; water color and transparency was low in immediate offshore area. The; cracking effect of the virioplankton on the host would affect the; abundance of picophytoplankton.国家海洋局公益性行业科研专

青海湖裸鲤的种群结构和线粒体DNA变异

对青海湖裸鲤55个个体Cytb基因全序列进行了测定和分析,探讨了种群结构和群体遗传多样性。用MEGA2.1软件分析了碱基组成和序列变异;以黄河花斑裸鲤为外群,构建了单倍型的NJ树;用Arlequin Ver.2 000程序计算了群体内遗传变异值(Fst)。结果显示,青海湖裸鲤群体没有显著的种群结构,提示青海湖裸鲤群体内存在广泛的基因交流;种群的遗传多样性较低(π=0.7828±0.0532),青海湖裸鲤种群很可能在历史上遭受过严重的“瓶颈效应”