丹江口水库鲤肠道寄生蠕虫群落结构与季节动态

2004年2月到2005年11月在丹江口水库库区206尾鲤(Cyprinus carpio)肠道中检获蠕虫11种,其中复殖吸虫3种,线虫5种,棘头虫2种,绦虫1种。总体感染率为45.63%,平均感染丰度为4.23±12.65,平均感染强度为9.29±17.48,其中饭岛盾腹吸虫(Aspidogaster ijimai)的总感染率(25.24%)和平均感染丰度(1.76±6.46)最大,瓣睾鲫吸虫(Carassatrema lamellorchis)的感染强度(25.00±46.68)最大。除部分平均感染丰度较低的线虫如鲤带巾线虫(Cucullanus cyprini)外,其他蠕虫的分布类型均为聚集分布,蠕虫群落多样性指数为4.63,均匀度指数为0.60,对群落多样性的季节动态分析表明,各季节群落多样性和均匀度波动较大,并无明显变化规律。每尾鲤感染蠕虫种数多在1—4种之间,所有感染的11种蠕虫中优势种为饭岛盾腹吸虫;次优势种为日本侧殖吸虫(Asymphylodora japonica)、中华许氏绦虫(Khawia sinensis)、瓣睾棘吸虫和鲤长棘吻虫(Rhadinarhynchus cyprini);非优势种为对盲囊线虫(Contracaecum sp.)、鲤带巾线虫、鲤杆咽吸虫(Rhabdochona cyprini)、黄颡刺盖线虫(Spinitectus gigi)、毛细线虫(Capillaria sp.)和木村小棘吻虫(Micracanthorhynchina motomurai)。在种间协调关系方面,鲤杆咽线虫和瓣睾鲫吸虫、鲤长棘吻虫和饭岛盾腹吸虫、对盲囊线虫和木村小棘吻虫、鲤长棘吻虫和木村小棘吻虫之间分别存在显著正关联。对优势种和次优势种蠕虫中种群的季节动态分析表明,鲤寄生蠕虫各组分的感染率和平均感染丰度存在显著的季节差异,在秋、冬季节的感染水平普遍比较高,而到春夏则急剧下降,但中华许氏绦虫无显著季节变化

西藏拉萨河鱼类内寄生蠕虫的种类组成及其群落特征

2004年7月对西藏拉萨河鱼类的体内寄生蠕虫进行了调查,共采集到7种鱼类,其中6种隶属于裂腹鱼亚科,1种隶属于鳅科。在这些鱼类中,共发现了2种棘头虫和1种棘头虫未定种、3种线虫、3种绦虫及异肉吸虫属(Allocreadium)的种类。它们分别是青海新棘吻虫(Neoechinorhynchus qinghaiensis)、裸鲤棘头虫(Echinorhynchus gymnocyprii)和新棘吻虫未定种(Neoechinorhynchus sp.),希蚋杆咽线虫(Rhabdochona hellichi)

武湖的渔业资源及渔业利用初步意见

武湖现有鱼类50种,其中鲤科鱼类33种,占总数的66%。渔获物组成中,鲢、鳙、鲤、鲫、鲌的产量占总产量的93%左右,渔获物以低龄为主。武湖目前的主要增养殖方式是放养鲢鳙,通过投放无机和有机化肥培养浮游生物来达到增加鲢鳙鱼产量的目的。针对武湖的渔业资源现状和渔业可持续发展的需要,提出了渔业利用的初步意见

Determination of DNA using Rivanol as the Fluorescent Probe in the Presence of SDS

建立了在SdS存在下， 以利凡诺（rVn） 为荧光探针测定dnA 的新方法. 在适量SdS存在下， rVn 的荧光被强烈地猝灭直至最低值. 但于上述rVn- SdS体系中加入dnA 后， 体系的荧光强度增强， 且荧光光谱发生移动. 在一定条件下， 荧光强度增强值与加入dnA 的量在一定浓度范围内呈现良好的线性关系.The quantitative method for DNA using rivanol(RVN) as the fluorescent probe in the presence of SDS was proposed.Under proper conditions,addition of DNA to a mixture of RVN and SDS resulted in enhanced fluorescence and spectral shifts of RVN-SDS system.The calibration graph was linear in the range of 0～10 μg/mL,the limit of detection was 62 ng/mL CT DNA

我国戊型肝炎疫苗和诊断试剂原始创新研究

戊型病毒性肝炎(戊肝)是全球最主要的病毒性肝炎之一,由戊型肝炎病毒(HEV)感染导致。戊肝多数呈自限性,孕妇罹患戊肝的病死率高达20%,慢性肝病合并戊肝、老年人戊肝症状较重、易导致肝衰竭。HEV主要通过肠道传播,常导致大的暴发流行,1986~1988年我国新疆发生一次国内外有文献记载的规模最大的戊肝大流行,共发

湖泊底泥中微囊藻DNA的分子检测

通过改进裂解温度和延长裂解时间并增加苯酚/氯仿洗脱次数的DNA提取方法获得南京玄武湖底泥中的DNA,通过PCR法来扩增微囊藻的16SrRNA基因．结果表明在所有采样点中均得到微囊藻基因组DNA,并且纯度较高,OD_(260)/ OD_(280)均高于1．54,最高值达到1．89．PCR的扩增结果显示所有样点的DNA都得到212 bp大小的微囊藻16SrRNA基因片断,表明这种方法可以有效的从底泥中提取微囊藻的DNA,从而为研究底泥微囊藻生理生态及其越冬、上浮、形成水华的机理提供更有利的方法

Preparation and Electrochemical Lithium Intercalation Performance of Segmented Carbon Nanofibers

以泡沫镍为催化剂 ,在 6 0 0和 70 0℃下 ,以CVD法热解乙炔气体制备大量的纳米碳纤维 .随着制备温度增加 ,纳米碳纤维直径变小 ,竹节状含量减少 ,d0 0 2 值减小 ,微晶片层平面Lc 和La 值增大 ,碳材料的可逆容量则下降 .分别用透射电镜、X射线衍射和拉曼光谱观察和测定了纳米碳纤维的形貌、微结构 ,发现在不同条件下生长的纳米碳纤维有不同的形貌和结构 .对纳米碳纤维的电化学嵌锂性能的研究表明 ,纳米碳纤维的结构对其电化学嵌锂容量和充放电循环寿命起重要影响 ,制备温度越低 ,纳米碳纤维的石墨化程度越差 ,可逆嵌锂容量相应要高一些Segmented carbon nanofibers were prepared by pyrolysis of acetylene on foam Ni at 600 and 700℃ in a fixed bed flowed-reactor. The morphology, microstructure and lithium insertion properties of these carbon nanofibers were investigated by TEM, XRD, Raman and electrochemical methods. Through TEM observations, it was found that this kind of carbon nanofibers was composed of lens-like segments with nearly equal separation stacking along the nanofiber axis. When the reaction temperature was 600℃, segmented carbon nanofibers were the major production. However, when the reaction temperature increased to 700 ℃, the content of segmented carbon filaments decreased and their diameter became smaller. The crystallite size d 002 and L c were determined by the 002 carbon Bragg peak of XRD patterns using the Bragg and Scherrer formulas. The intensity ratios of the 1350 cm -1 line and the 1580 cm -1 line (R=I D/I G) was used to evaluate the L a value, which was inversely proportional to the effective crystallite size in the direction of the graphite plane (L a). With the reaction temperature increased, the d 002 value decreased, L a and L c values increased, which indicated the degree of crystallinity increased. Segmented carbon nanofibers were used as positive electrodes of C/Li cells. The first charge capacities of C/Li cells were 480 and 300 mAh/g for samples produced at 600 and 700℃, respectively. The samples at 600℃ showed capacities higher than the theoretical value of graphite, which was attributed to accommodation of more lithium at the edge of graphene layers and on the surface of graphene layers according to the mechanisms of lithium insertion in carbons prepared by low-temperature pyrolysis of hydrocarbons. As confirmed by the XRD and Raman spectra, the samples at 700℃ had larger L a and L c, which led to the capacity decreasing.国家自然科学基金 (6 0 2 710 0 9);; 浙江省自然科学基金 (5 0 110 9,2 0 0 0 5 3)资助项

ANALYSES ON LDH ISOENZYME AND PROTEIN FROM BOTH GUANGDONG STRAIN AND FUJIAN STRAIN OF ECHINOCHASMUS FUJIANENSIS

目的　探讨福建与广东两地福建棘隙吸虫生化特点。方法　用不连续聚丙烯酰胺凝胶电泳及薄层等电聚焦电泳等进行比较分析虫体乳酸脱氢酶 (LDH)同工酶和蛋白质。结果　福建与广东两地虫株都有 5种LDH同工酶 ,其中LDH1、LDH4 和LDH5的迁移率相同 ;蛋白质电泳都分离出 9条区带 ,其中有 7条带的等电点相同。但在LDH2 和LDH3 的迁移率 ,蛋白质区带的相对含量 ,同工酶的总活力与比活力 ,以及A和B亚基的含量等存在差异。结论　LDH同工酶与蛋白质的某些差异 ,可归因为基因型相似条件下所出现两地虫株生化遗传上差异Aim\ Inguired into the biochemical characteristrics of Echinochasmus fujianensis found in Fujian and Guangdong provinces. Methods\ Compared and analysed two strains by LDH isoenzyme eletrophoresis, discontinuous PAGE and thinlayer isoelectric equilibrium PAGE.Results\ Two strains both had five LDH isoenzyme, among them, LDH\-1、LDH\-4 and LDH\-5 had the same mobilities. There were nine bands in protein electrophoresis. Seven bands had the same isoelectric point.But there were differences in the mobilities of LDH\-2 and LDH\-3,the comparative amount of protein bands, total and specific activity of isoenzyme and the amount of A and B subunit. Conclusion Under the similar condition of genotypes, the differences of the biochemical inheritance can be contributed to the differences of LDH isoenzyme and protein.福建省科技计划基金资助项目!(No.98-Z-156);; 福建省“百千万人才”人选培养资金资助项

戊型病毒性肝炎研究进展

戊型病毒性肝炎是全球最主要的病毒性肝炎之一,近半个世纪多次发生大规模暴发,孕妇感染戊型肝炎后病死率高达20%.近年随着基于构象型表位多肽E2的戊型肝炎诊断试剂的出现,戊型肝炎的病原学诊断及流行病学调查均获得了较大的发展,越来越引起人们的重视.由我国自主创新研制的重组戊型肝炎疫苗现已在中国完成世界上首个Ⅲ期临床试验,其预防戊型肝炎的保护率达到100%(95%CI,72.1%~100.0%)

SARS冠状病毒核衣壳(N)蛋白不同区域的原核表达

利用大肠杆菌表达系统对SARS冠状病毒的核衣壳(N)蛋白全长及N末端或/和C末端缺失突变体进行了表达,共表达了39个重组蛋白,表达量在15%～30%之间。分别利用电洗脱或金属鳌合介质纯化重组蛋白,用蛋白印迹实验检测纯化蛋白对SARS病人恢复期血清的反应性,结果发现全长N蛋白活性最好,其余的末端缺失蛋白均无法达到同一活性水平。由此说明N蛋白的完整性对于其优势表位的充分暴露是必要的