Expression and test of the neutralization Fab antibody against infectious bursal disease virus

为表达抗鸡传染性法氏囊病病毒(IbdV)抗体fAb并检测其中和活性,本研究将抗IbdV抗体的轻链(l)和重链片段(fd)基因分别克隆于P ET-27b(+)载体中,并转化于大肠杆菌rOSETTA(dE3)进行诱导表达。将l和fd片段包涵体蛋白变性后等量混合于复性液中,制备fAb并对其进行活性鉴定。结果显示l和fd蛋白相对分子质量大小分别为25 ku和28 ku。WESTErn blOT和ElISA检测结果表明,获得的抗体fAb大小约为50 ku,并且与VP2蛋白和不同病毒株均具有特异性结合能力。体外中和试验结果表明,获得的IbdV抗体fAb具有中和活性,可以有效阻断IbdV(b87株)对鸡胚成纤维细胞(df1)的感染。本实验获得的IbdV抗体fAb有望成为治疗Ibd的候选生物制剂,为研制治疗Ibd抗体制剂奠定了基础。To express the neutralizing Fab antibody against infectious bursal disease virus(IBDV),the gene of light chain(L)or heavy chain fragment(Fd) against IBDV was cloned into the prokaryotic expression plasmid,respectively,and then the recombinant L or Fd was expressed in E.coli Rosetta(DE3),respectively,and purified through sole denaturation and co-renaturation of inclusion body.Western blot results showed that the Fab was approximately 50 ku.ELISA results showed that the Fab exhibited binding ability and specify to VP2 for different IBDV strains.The results of neutralization test in vitro showed that the Fab exhibited neutralizing activity to IBDV-B87 strainin chicken embryo fibroblast(DF1) cells.The Fab antibody prepared in this study is expected to become a candidate drug for treatment of IBD,which laid the foundation for the treatment of IBD.黑龙江省应用技术研究与开发计划项目(GC13C104

AN ORDER REDUCTIOX SEARCH ALGORITHM FOR DEAD TIME OF DELAY PROCESS

本文讨论了在特殊信号下,非真阶非真滞后模型参数的最小二乘估计与纯滞后之间的关系,提出了一种新的辨识纯滞后的方法——降阶搜索算法

花生红衣素定性试验及稳定性研究

对花生红衣素的性质及其稳定性进行研究，结果表明，花生红衣素为兼有酮、酚性质的物质，耐光、耐热、耐酸不耐碱、耐还原剂（Na2SO3）不耐氧化剂（H2O2），在Zn^2＋、Fe^2＋、Fe^2＋、Cu^2＋离子条件下不稳定，而对K^＋、Mg^2＋、Ca^2＋离子则稳定，对蔗糖、葡萄糖和食盐等常用添加剂和对羟基苯甲酸甲酯、苯甲酸钠等防腐剂表现出较好的稳定性

Morphological changes of major gulfs along the coast of China from 2010 to 2020

Based on Landsat images,the spatial information of major gulfs along the coast of China in 2010,2015,and 2020 were extracted to explore the morphological change characteristics.Some evaluation indexes,such as shoreline utilization degree,gulf area,gulf shape index,and gulf centroid,were used to analyze the gulfs' spatio-temporal change features in detail.The research showed that:(1) The shoreline length and structure of major gulfs changed significantly between 2010 and 2020,and the length and retention rate of the natural shorelines decreased.(2) The development and utilization degree of the gulf increased,with the increase rate gradually slowing down,and most of the gulf was moderately developed.(3) The gulf area continued to shrink,with a slow-down shrinking rate,and the gulf area in the Bohai Sea shrunk the most and the fastest.(4) The morphological change trend of the gulf is different from north to south.The north tends to be complicated,while the south to be simple.(5) After 2015,the gulf's plane center of gravity tended to be stable,the displacement distance decreased,and the displacement velocity slowed down.Human activities,such as sea reclamation,are the dominant factors leading to gulfs' morphological change.The national policies and measures for sea reclamation management and control in recent years have initially shown their effectiveness in affecting the gulf's morphology changes.The gulfs along the coast of China should be functionally positioned and divided,with refined classified management to optimize the layout of the Ecological-Production-Living space in the gulf areas

Fractal Dimension Characteristics of Chinese Mainland Coastline Based on Automated Divider Method:Comparative Analysis with Box-Counting Method

Fractal dimension is a fundamental index used to describe the complexity of coastlines.However,the traditional method of calculating divider dimensions using hand-drawn circles is no longer suitable for studying coastlines at large spatial scales due to its limited accuracy and lack of convenience in the current information age.Therefore,this paper developed an automated software based on the principle of the divider method,using the open-source PyQGIS.This software accurately and conveniently calculated the divider dimensions of Chinese mainland coastline,various sea area coastlines,and different province coastlines.Furthermore,the divider dimensions calculated by the software were compared and analyzed with the box-counting dimensions obtained through the box-counting method.It is found as follows.① Compared with the traditional hand-drawn divider method,the automated divider method can ensure computational speed and accuracy while extending the calculation scale,and the obtained divider dimension is generally higher than the box-counting dimension obtained by the box-counting method.②The divider dimension of Chinese mainland coastline in 2020 is 1.194 8,while the divider dimensions of the north and south of Hangzhou Bay are 1.170 4 and 1.217 5,respectively,indicating a higher complexity in the coastline on the south of Hangzhou Bay.③The fractal dimension of Chinese mainland coastline exhibits substantial spatial variations,showing a pattern of higher values in the north and south and lower values in the middle.At the sea area level,the divider dimension of the coastline is the largest (1.209 1) in the South China Sea area and the smallest (1.156 3) in the South Yellow Sea area.At the provincial level,the divider dimension of the coastline is the largest (1.290 4) in Tianjin and the smallest (1.087 5) in Shanghai.This study demonstrates that the automated divider method offers a convenient and robust calculation tool for analyzing the fractal dimension of coastlines.It enhances computational efficiency and accuracy while providing technical support and serving as a reference for decision-making in the investigation and comprehensive management of coastlines

气候变化和人类活动对滨海湿地的影响研究进展

On the influences of runoff, sediment and marine dynamics, the coastal wetlands keep a high silt / recession dynamic, typical vegetation succession, dramatic landscape changes and consequently present as an extremely fragile ecosystem. In recent dec ades, coastal wetlands are facing increasingly serious risks of degradation and loss due to the impacts of global climate change and hu man activities. In this paper, the key factors affecting the coastal wetlands and their effects, and the impacts of sea level rise caused by climate warming and of increasing frequency human activities driven by economic development on coastal wetlands are reviewed. On this basis, the issue and frontier in this scientific field are put forward. What’s more, the strategic point that it needs to take into ac count the dual stress both from the land and the sea to predict the future “fate” of coastal wetlands is emphasized, which should be not only considering the long-term trends of population, economic development, social progress and climate change, but also paying atten tion to the mutual feedback mechanism and synergistic effect among the key factors affecting the evolution of coastal wetland

含镍合金钢用Mg-Al-O高温防护涂层作用机理研究

采用空气喷涂法在SPA-H含镍合金钢表面制备了Mg-Al-O高温防护涂层,研究了涂层对SPA-H合金钢的高温氧化行为及氧化皮粘附性的影响。采用金相显微镜对喷涂样和空白样的剖面和表面形貌进行表征,采用SEM和EDX分析氧化皮中元素分布,研究Mg-Al-O涂层的作用过程和防护机理。结果表明:涂层可以大幅度提高SPA-H合金钢高温耐氧化腐蚀能力,在1 100℃、1 200℃、1 250℃下恒温2 h,降低氧化烧损分别为47.2%、41.3%、41.5%。涂层在钢坯表面和基体生成的氧化皮发生反应,生成尖晶石结构,抑制阳离子扩散速率。同时通过减少基体和涂层界面处Ni、Cr氧化物的生成量,改善热轧前氧化皮...</p

Residential Demand Side Response Considering Energy Storages and Renewable Energy Sourced Generators

传统居民侧需求响应策略很少考虑储能和新能源发电环境，难以适应新的发展趋势和遇到的新挑战，如新能源发电的随机性对需求响应策略带来风险成本，电池储能系统的缓冲效果增加了模型求解的复杂度。针对上述问题，研究了含储能及新能源发电的居民侧需求响应策略。考虑了新能源发电随机性和电池缓冲作用，建立了居民侧两阶段随机规划需求响应模型以降低预测误差对需求响应策略带来的风险成本。此外，在模型精度和计算复杂度之间进行了折中，使模型不依赖于复杂的智能算法即可快速求解。通过与不考虑随机性的需求响应策略对比，验证了本文提出的需求响应策略的有效性