Core-shell Hydroxyapatite Combined with Bone Morphogenetic Protein 2 in Periodontal Regeneration Treatment in Dogs

目的初步评估贝壳多孔羟基磷灰石基骨修复材料及该材料和骨形成蛋白-2联合应用引导比格犬牙周组织再生的效果。方法选取18月龄比格犬6只,牙周基础治疗后1周,在下颌第二、三、四前磨牙,建立急性牙周骨缺损模型,依照分组情况进行不同治疗。实验组(T组)植入骨修复材料和骨形成蛋白-2;阴性对照组(nC组)植入骨修复材料;空白对照组(bC组)不植入任何材料。实验设计采取同颌同名牙对照,同一只比格犬的3对同颌同名牙分别为:空白对照组和阴性对照组,阴性对照组和实验组,空白对照组和实验组。术后12周,处死动物,MICrO-CT检查并对数据进行统计学分析。结果材料植入后,未见材料溢出,植入局部和全身都未见明显不良反应。3组缺损都有一定程度骨再生,以T组再生组织量最多,bC组最少。MICrO-CT结果显示:T组、nC组和bC组的骨再生平均高度为(4.50±0.47)MM(、1.75±0.42)MM和(0.87±0.31)MM。nC组和bC组相比,差异有统计学意义(P<0.05)。T组与nC组和bC组相比,差异均有统计学意义(P<0.05),且有临床意义。结论贝壳多孔羟基磷灰石基骨修复材料和骨形成蛋白-2联合应用于比格犬,可以获得更好的引导组织再生效果。Objective To evaluate the ability of core-shell hydroxyapatite bone graft material alone and combined with bone morphogenetic protein 2(BMP-2) in periodontal regeneration treatment in dogs.Methods Thirty-six defects were created in six 18-months male beagle dogs at the sites of the second,third and fourth mandibular premolars one week later after the dogs were treated with non-surgical periodontal therapy.Different treatments were carried out according to which group the teeth belonged.There are 3 groups.The test group(group T) was treated with core-shell hydroxyapatite bone graft material combined with BMP-2;the negative control group(group NC) was treated with core-shell hydroxyapatite bone graft material alone and the blank control group(group BC) was treated with no graft material.When a tooth was selected into one of the 3 groups randomly,the tooth with the same name on the other side in the same jaw was selected into another group.6 defects in the same dog were made and divided into 3 groups.The animals were sacrificed 12 weeks after surgery and analyzed by Micro-CT.Results There was no adverse reaction after material was planted.There were bone regenerations in all groups.Group T got the best bone regeneration while group BC got the least bone regeneration.The biggest height of bone regeneration for group T,group NC and group BC were(4.50±0.47) mm、(1.75±(0.42) mm) and(0.87±0.31) mm according to Micro-CT evaluation.There were statistical difference of bone regeneration height between group NC and group BC.There were statistical differences between group T and group NC and between group T and group BC.Conclusion More periodontal tissue regeneration could be got when core-shell hydroxyapatite bone graft material was used with bone morphology protein 2.南京市医学科学技术发展专项项目(ZKX07022);天津市生物医学材料重点实验室开放课题;南京市医学科技发展青年人才启动项目(QYK10166

子宫内膜异位症小鼠巨噬细胞移动抑制因子变化规律的研究

利用BALB/c小鼠子宫内膜异位症(EMs)模型,采用实时荧光定量PCR技术和酶联免疫吸附试验,从mRNA水平和蛋白水平上检测了EMs BALB/c小鼠腹腔免疫细胞和腹腔液中的巨噬细胞移动抑制因子(MIF)在造模后变化情况.结果显示:造模后的21 d内MIF在基因水平和蛋白水平上都高于对照组,尤其在异位灶形成的早期阶段增加更为显著.提示MIF与EMs的发生发展过程关系密切,可能参与早期的病理活动过程

一种基于微电极的珊瑚共生体光补偿点测定方法探讨

珊瑚虫与虫黄藻互利共生形成共生体,虫黄藻通过光合作用为珊瑚虫提供重要的能量来源。珊瑚共生体的光补偿点可以较好地指示珊瑚的光适应性,是重要光合特性指标,但其测定方法鲜有报道。利用溶氧微电极结合光强可调节光源,以鹿角杯形珊瑚为实验材料,依据扩散平衡理论,建立了一种造礁石珊瑚的光补偿点的测定方法。实验结果表明珊瑚的扩散边界层溶氧浓度会快速响应光强变化。珊瑚扩散边界层溶氧浓度与光合有效辐射具有较好的相关性,且光强由高降低过程测得的相关系数更高。实验测得鹿角杯形珊瑚的光补偿点较低,为1.52μE/m~2/s,与喜阴植物的光补偿点相当。相对较低的光补偿点利于珊瑚栖居于水深范围更广的区域,有助于扩展珊瑚的生态位。溶氧微电极相关技术和方法在珊瑚光合作用研究中具有广泛的应用前景。中国科学院战略性先导科技专项A(XDA13020300)国家自然科学基金(41676163; 41406191; 41276113; 41276114)国家重点研发计划(2017YFC0506301,2018YFC1406500)广州市珠江科技新星(201806010017