Chemical constituents and cytotoxicity assay research in small polar substances from Vitis thunbergii var. taiwaniana

该文对小叶山葡萄地上部分化学成分进行了研究,运用硅胶柱色谱,OdS中低压柱色谱,SEPHAdEX lH-20凝胶柱色谱,分析型和制备型HPlC从小叶山葡萄60%乙醇提取物大孔树脂95%乙醇-水洗脱部位分离得到12个化合物,利用高分辨质谱,核磁共振等波谱手段鉴定其结构分别为:(1)bETulInIC ACId,(2)2,2,2'-bIS(4-HydrOXyPHEnyl)PrOPAnE bIS(2,3-EPOXyPrOPyl)ETHEr,(3)ErIOdICTyOl,(4)TrAnS-ε-VInIfErIn,(5)(+)-CIS-ε-VInIfErIn,(6)kObOPHEnOl A,(7)AMPElOPSIn A,(8)nEPAlEnHEnOl,(9)CIS-MIyAbEnOl C,(10)CIS-VITISIn b,(11)CIS-gnETIn H和(12)(+)-HOPEAPHEnOl。化合物2,5,6,8,9,10,11均为首次从为葡萄属中分离得到的化合物,化合物3,7,12为首次从小叶山葡萄中分离得到的化合物。在作用浓度为50μMOl·l-1下,化合物6,7和11对MCf-7(乳腺癌细胞株)具有较明显的体外生长抑制作用,其抑制率分别为66.58%,57.16%,52.84%。This article studied the chemical constituents from the aerial part of Vitis thunbergii var.taiwaniana.The 60% ethanol extract was eluted with 95% ethanol though HP-20 macroporous adsorption resin column.12 compounds,including( 1) betulinic acid,( 2) 2,2,2'-bis( 4-hydroxyphenyl) propane bis( 2,3-epoxypropyl) ether,( 3) eriodictyol,( 4) trans-ε-viniferin,( 5)( +)-cis-ε-viniferin,( 6) kobophenol A,( 7) ampelopsin A,( 8) nepalensinol B,( 9) cis-miyabenol C,( 10) cis-vitisin B,( 11) cis-gnetin H and( 12)( +)-hopeaphenol,were separated by using normal phase silica gel,ODS,Sephdadex LH-20 column chromatographies and semi-preparative or preparative HPLC.Compounds 2,5,6,8,9,10,11 were separated from the genus Vitis for the first time and compounds 3,7,12 were separated from Vitis thunbergii var.taiwaniana for the first time.At a concentration of 50 μmol·L-1,compound6,7 and 11 showed strong cytotoxicity against MCF-7 cell lines with the inhibition rate of 66.58%,57.16%,52.84%,respectively.国家自然科学基金青年基金项目(81202419

ゼミ・実習研究発表会報告概要

社会学部設立40周年記念特

ヤセイ ドウブツ カンリ ノ タメノ フィールド チョウサホウ : ホニュウルイ ノ コンセキ ハンテイ カラ データ カイセキ マデ

希少種の保護や過増加した在来種・外来種の対策など、野生動物をめぐるさまざまな課題に応えるフィールド調査法。各動物の食性や個体数に関する既存研究をまとめ、地図の読み方やフィールド機材の使い方、糞や足跡をはじめとする動物の痕跡の識別法を具体的に示し、得られた情報から食性や個体数、生息地を評価する方法を体系的に解説する

[[alternative]]The Role of Images and Symbols in Poster Designing

[[abstract]]Since the beginning of human life in the universe, humans have learnt to understand the surrounding environment and matters surrounding them, the predictable and unpredictable phenomena, such as what food are edible, what animals are dangerous, what kind of weather bodes rain, etc., for environmental adaptation and life preservation purposes. Humans learnt to control what they understood and left what they don't understand to the hands of the spiri- tual being. They gradually invented gadgets and things to make life easier, used sounds to communicate with their peers, as well as tattooed their bodies with status symbols and images that empowered them with strength. As life progressed, each object gradually began to have meaning to these humans. Let us take for instance the significance of a deer to the primitive people. It has edible meat, fast legs, beautifully printed skin that could provide warmth to the body, and dangerous antlers. Hence the deer signified food, speed, beauty, clo-thing, glory, and danger, etc. In the cave wall drawings, the deer was a symbol of a prayer for food or personal wealth. How- ever, as humans accumulated more living experiences and civilization developed the mean- ings of images took a change. For instance, the deer on a Valentine's card signifies love, but the deer sign beside a suburban road means watch out for animals. In posters, the deer also stopped symbolizing food or wealth, a deer on a poster calls for the protection of endangered species. It is quite apparent that the symbolic meaning of objects is representative of life, it ch- anges with time, people and other factors. Sometimes, these symbols would gradually beco- me forgotten, but sometimes, they become internationally recognized symbols. Hence, the paper shall delve into the characteristics of object symbols. How object becomes a poster image, how to expand the width and depth of an image symbol, how they come to signify the meaning they portray, and so forth. Findings of the study shall be used in actual poster crea- tion and future design education.

含水生物样品的环境扫描电镜观察

目前，在常规的扫描电镜中观察含水的生物样品有两种方法：（１）用戊二醛和锇酸固定细胞的骨架，再用乙醇脱水，二氧化碳临界点干燥后喷金，然后放入扫描电镜观察。（２）样品深度冷冻后使用冷冻样品台放入扫描电镜观察。以上两种制备方法都会使样品失去原来的自然面貌。..