Study on the Biology of Eimeria tenella from Xiamen and the Effect of Alage Transformed with Thymosin α1 Gene for Anti-Coccidia

摘要2001年8月份至2004年7月份，作者对厦门产的柔嫩艾美尔球虫（E.tenella）的病原生物学、生活史各期形态特征、球虫的致病作用等方面进行了详细的研究，在此基础上对鸡感染从厦门获得的柔嫩艾美尔球虫{以下简称柔嫩艾美尔球虫（厦门）}后血清抗体变化规律也进行了考察，并对转胸腺素α1基因蓝藻在球虫感染中的保护作用及药代动力学进行探讨，研究内容结果如下：一、柔嫩艾美尔球虫厦门（E.tenella）病原生物学考察，此项内容包括：1.采用单卵囊分离技术分离并增殖到柔嫩艾美尔球虫（厦门），建立了用蔗糖梯度离心的方法纯化球虫卵囊的方法。2.E.tenella（厦门）内生发育阶段的各期形态的研究包括采...AbstractThe present paper deals with the morphology ，developmental cycle and pathogenicity of E.tenella from Xiamen， the paper also deals with the immune response of chicken to E.tenella ,and study on the effect of algae transformed Tα1 gene for anti-coccidia，the results as follows: 一、The study on the biology of E.tenella from Xiamen，it deals with the following contents: 1. The E.tenel...学位：理学博士院系专业：生命科学学院生物学系_动物学学号：B20012600

Preventive and Therapeutic Effects of Camel Milk on Type 2 Diabetes Mellitus

探讨骆驼奶对2型糖尿病预防和治疗作用。采用预防性灌胃骆驼奶后在造模和先造模再治疗性灌胃骆驼奶两种方法,分别与对照组、糖尿病模型组、牛奶组、盐酸二甲双胍组和驼奶与盐酸二甲双胍混合组比较,研究骆驼奶对由高脂饮食联合链脲佐菌素(STrEPTOzOTOCIn,STz)诱发2型糖尿病模型小鼠的预防和治疗作用,并测定其空腹血糖(gluCOSE)含量、血清超氧化岐化酶(SuPErOXIdE dISMuTSE,SOd)活性、谷胱甘肽过氧化物酶(gluTATHIOnE PErOXIdASE,gSH-PX)活性、丙二醛(MAlOn dIAldEHydE,MdA)含量及甘油三酯(TrIglyCErIdE,Tg)、总胆固醇(TOTAl CHOlESTErOl,TC)、高密度脂蛋白胆固醇(HIgH dEnSITy lIPOPrOTEIn CHOlESTErOl,Hdl-C)、低密度脂蛋白胆固醇(lOW dEnSITy lIPOPrOTEIn,ldl-C)的含量。结果表明:骆驼奶能显著降低2型糖尿病小鼠空腹血糖,增加其血清SOd、gSH-PX活性和Hdl-C含量,降低血清MdA、Tg、TC、ldl-C含量。因此,骆驼奶有较好的预防和治疗2型糖尿病的作用。In this study,two ways were used to investigate and explain the preventive and therapeutic effects of camel milk on type 2 diabetes mellitus.One way was to feed the C57BL /6 mice with camel milk and high fat diet for 35 days,then the mice were intraperitoneally injected with Streptozotocin; and in another way,C57BL /6 mice were given high fat diet for 35days,followed by intraperitoneally injection with streptozotocin to establish type 2 diabetes mellitus models,and then the mice were fed with camel milk.Finally the SOD and GSH-Px activity,blood glucose level,and malon dialdehyde( MDA),triglyceride( TG),total cholesterol( TC),HDL-C,LDL-C were determined in the control group,diabetes mellitus model group,milk group,metformin hydrochloride group and camel milk and metformin hydrochloride mixed group.The results showed that camel milk can significantly reduce the glucose level,MDA,TG,TC and LDL-C contents in blood,and enhance the SOD and GSH-Px activity,and HDL-C content of diabetes mellitus mice.Therefore,camel milk has preventive and therapeutic effects on hyperglycemia and hyperlipidemia,and can effectively enhance the anti-oxidation ability of type 2diabetes mellitus mice.新疆维吾尔自治区科技支疆项目(201191163); “新疆特殊环境物种多样性应用与控制”重点实验室资

Raise of the Frequency in Green-shoot-formation for Rice Anther Cultivation

福建省水稻攻关课题基金 (K790 81);; 厦门市科委科技发展项目 ( 3 5 0 2 2 2 0 0 0 10 4

Effect of Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD-gene on antioxidation activities in mice

目的研究转人铜锌超氧化物歧化酶(hCu,Zn-SOD)突变基因聚球藻口服后的生物活性。方法给小鼠灌服转hCu,Zn-SOD突变基因聚球藻20d,然后测定小鼠谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量。结果转hCu,Zn-SOD突变基因聚球藻可明显提高小鼠血清GSH-Px活力和全血血红蛋白CAT活性,显著提高小鼠血清和肝脏中Cu,Zn-SOD和总SOD(T-SOD)的活力,显著降低小鼠血清和肝脏的MDA含量。结论转hCu,Zn-SOD突变基因聚球藻有较强的抗氧化作用。Objective To study the biological activities of Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD-gene.Methods Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD were administered orally for 20d to mice,then the activities of glutathione peroxidase(GSH-Px),catalase(CAT),superoxide dismutase(SOD) and the content of malondialdehyde(MDA) were determined.Results The activities of GSHPx in serum and the activities of CAT in blood increased obviously;the activity of SOD in serum and liver increased markedly;the content of MDA in serum and liver decreased obviously.Conclusion Synechococcus sp.PCC7942 with trans-mutated hCu,Zn-SOD-gene had obvious antioxidant effect in vivo.福建省自然科学基金资助项目(C0510004

Studied the difference of genetic feature between tissue cultured and cultivated paddy rice of Jia He Zao Zan via microsatellite(SSR) markers

株高是水稻重要的农艺形状之一,植株过高将导致倒伏和减产,目前,很多新的技术究被用来鉴定,图位克隆与水稻株高相关的基因及机理的研究,本实验选择优质早籼稻佳禾早占种植苗和经过组培获得的矮化突变水稻为材料,为研究比较它们间的遗传物质上差异,根据康耐尔大学的资料设计了311对SSR引物对佳禾早占种植材料和组培材料进行分析,对两种材料进行PCR多态性扩增,结果发现两者间存在多态性的引物有88对,多态性比例达到30.3%。在矮杆材料中不但验证了已报道的11个与调控株高性状基因相连锁的标记连锁群,同时在第3号染色体和第9号染色体上还获得了两个以前基本未有报道的标记集中分布区域。结果说明,该培养基培育出的佳禾早占水稻后代所表现出的矮杆性状与亲本在遗传物质上确有明显差别。该结果有助于挖掘和定位新的矮杆基因,并有利于今后在水稻育种中进行水稻株高性状的控制,同时也为开展矮化性状机理的研究提供有利的实验材料。Plant height is one of the important agronomic traits of rice.Overhigh plant easily led to lodge and reduce output.Recently,great advances have been made in the identification,mapping,cloning and action mechanism of plant height genes in rice.This present paper selected the tissue cultured and cultivated paddy rice of Jia He Zao Zan(J.H.Z.Z) as experimental object,which were significant different on the height,the tissue cultured plant was only less than half height of the cultivated plant.In order to compare their differences of genetic and determine whether there are relationship between the dwarf gene and the dwarf character of tissue cultured plant,Polymorphism analysis was conducted between them by 311 pairs of rice SSR primers,designed by Cornell University,the result indicated that 88 pairs of primers had polymorphism between two kinds of materials,the ration in total primers was about 30.3%,the experimental result not only verified 11 multiple groups which regulate the height gene,but also obtained two group regions which control the dwarf character on chromosome 3 and chromosome 9,which have not been reported previously,these results will help to explore and determine the situation of new dwarf gene,and will be of benefit to act genetic manipulation of plant height of in rice breeding.These results also provided the evidence for studying the mechanism of dwarf.国家863项目(2002AA211091),(20001AA11091);; 厦门大学细胞生物学和肿瘤细胞工程教育部重点实验室开放基金资助(NO:2005108)资

福建九牧耕牛感染吸虫情况的粪检调查

福建省北部山区是几类重要吸虫的流行区。１９９３年５～６月间，用重力沉淀法对闽北九牧乡的１１７１头耕牛进行粪检调查，结果表明：水牛的胰吸虫感染率为０，前后盘吸虫感染率为９６．０５％，肝片吸虫感染率为３８．１６％；黄牛的胰吸虫感染率为５３．１９％，前后盘吸虫感染率为９３．６２％，肝片吸虫感染率为３４．０４％。另外，对中间宿主的感染情况也进行了剖检。所得结果对认识吸虫在自然界流行的保持机制，进行防治有重要意义

A study on antioxidation of synechococcus sp. PCC 7942 withTrans-thymosin α1-gene in Mice

本试验室已在蓝藻聚球藻中高效表达了人源胸腺素α1(thymosinα1,Tα1)基因,为研究转Tα1基因聚球藻口服后的生物活性,本研究给小鼠灌服转Tα1基因聚球藻14d,研究其对小鼠谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(Cat)和超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量的影响,结果表明:转胸腺素α1基因聚球藻可显著提高小鼠心、肝与肾中GSH-Px活力(P<0.01);明显提高心脏Cat活性(P<0.01);显著降低肝脏中MDA的含量(P<0.01);但对SOD活力无明显作用。提示转胸腺素α1基因聚球藻较强的抗氧化作用。Human thymosin α1 gene was expressed effectively in Synechococcus sp. PCC 7942 and antioxidant effect of Synechococcus sp. PCC 7942 with trans-thymosin α1-gene in mice were investigated. Synechococcus sp. PCC 7942 with trans-thymosin al-gene were administrated orally 14d,Laters the results showed that the activities of glutathione peroxidase (GSH-Px) in heart、liver and kidney were increased significantly (P<0. 01);the activity of catalase (Cat) in heart was increased markedly;the content of malondialdehyde (MDA) in liver was decreased obviously (P<0.01). But no significant change in the activity of super-oxide dismutase (SOD) was observed. It indicated that Synechococcus sp. PCC 7942 with trans-thymosin α1-gene had obvious antioxidation in vivo.国家海洋863课题(项目编号:819-04-03);福建省自然科学基金项目(项目编号:c0010002

胸腺素原体内抑制小鼠肝癌细胞H22的研究

根据PArkIn等[1]报道,2000年全球新发癌症病例为1 010万例,死亡620万。在fErlAy等[2]报道中最常见的癌症中致死率排列第三是肝癌。在我国肝癌的死亡率占全部恶性肿瘤死亡的18.8%,仅次[3]厦门市科技项目(3502Z20083004);国家“973”项目(2007CB513103);福建省医药卫生创新项目(2009-CXB-51)资

End-stage chronic renal failure in patients treated by antireflux operation

全例逆流性腎症によると考えられる蛋白尿を術前から有していた.1例ではPaquin法, 2例ではPolitano-Leadbetter法が施行されていた.腎機能の急速な悪化は, 全例で思春期以降で, 2症例では初診時すでにsmall kidneyが認められていたWe report 3 patients progressed to end-stage chronic renal failure after antireflux surgery. Preoperatively, the 3 patients had proteinuria, probably due to reflux nephropathy. Surgical procedures used were Paquin's method in 1 patient and Politano-Leadbetter's method in 2. Rapid deterioration of renal function was observed from puberty in all patients. In addition, renal hypoplasia was suspected in 2 patients. We concluded that both reflux nephropathy and renal hypoplasia played important roles in the progression of renal insufficiency