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首都大学東京, 2017-03-25, 修士（文学）首都大学東

Effects of Shenling Baizhu Powder on expression of apoptosis related proteins of tumor chemotherapy model mice with H22 hepatocellular carcinoma cells

目的:研究参苓白术散(SLBZP)对H22肝癌移植瘤小鼠化疗后肿瘤凋亡相关因子Caspase -3、; Caspase-9、X染色体连锁凋亡抑制因子(XIAP)、血小板源生长因子-BB(PDGF-BB)、血管生成素-1(Ang-1)、血管生成素-2; (Ang-2)表达的影响。方法:SPF级昆明小鼠64只,将肝癌H22细胞悬液(2*10~7/mL)接种于小鼠右前腋皮下,1周全部形成移植瘤后,行; 环磷酰胺(CTX,0.20g/kg,0.2mL/10g)腹腔注射,建立肿瘤化疗模型。随机分组为8组:模型组,CTX(0.02g/kg)组,SLB; ZP高、中、低(6.00、3.00、1.50g/kg)剂量组,SLBZP高、中、低剂量+CTX(0.02g/kg)组,给药14d后,运用蛋白印迹; 法(Western blot)测定小鼠瘤组织中Caspase-3、; Caspase-9、XIAP蛋白表达;酶联免疫吸附(ELISA)测定小鼠血清PDGF-BB、Ang-1、Ang-2表达。结果:SLBZP联合治疗; 各组抑瘤作用明显,CT X组,SLBZP高剂量组,SLBZP高、中剂量+C T X组抑瘤率分别为52.39%、; 45.84%、58.41%、52.77%。与模型组比较,各治疗组血清PDGF-BB、Ang-1、Ang-2及瘤组织中XIAP表达呈现出被下调的趋; 势,瘤组织中Caspase-3、Caspase-9表达呈现出被上调的趋势,且以SLBZP高剂量加CTX组变化幅度最为显著(P<0.01,P<0.; 05)。结论:SLBZP联合化疗可以更有效调节H22肝癌移植瘤小鼠肿瘤凋亡相关因子的表达,促进肿瘤细胞凋亡可能是其改善化疗的机制之一。Objective: To investigate the effects of Shenling Baizhu Powder (SLBZP); on expression of apoptosis related proteins such as Caspase-3,; Caspase-9, X-linked inhibitor of apoptosis (XIAP), PDGF-BB, Ang-1, Ang-2; of tumor chemotherapy model mice with H22 hepatocellular carcinoma; cells. Methods: Sixty-four Kunming mice were subcutaneously injected; with suspension of H22 hepatocellular carcinoma cells (2*10~7/mL) into; the right anterior armpit. After 1 week, all transplanted tumors were; formed and the mice were received intraperitoneal injection with cytoxan; (CTX) with the dosage of 0.2g/kg to establish the tumor chemotherapy; model. Then mice were randomly divided into eight groups. CTX group was; treated with CTX (0.02g/kg), the model group was treated with; physiologic saline, three SLBZP groups were treated with SLBZP (6.00,; 3.00, 1.50g/kg), and other three groups were treated with SLBZP (6.00,; 3.00, 1.50g/kg) plus CTX (0.02g/kg). After all groups were treated for; 14 days, Western blot was used to detect the expression of Caspase-3,; Caspase-9 and XIAP proteins in tumor tissue, and protein expression of; PDGF-BB, Ang-1, Ang-2 in blood was measured by enzyme-linked; immunosorbent assay (ELISA). Results: The combined treatment group had; obvious effect on tumor inhibition. Tumor inhibitory ratios in CTX; alone, SLBZP (H), SLBZP (H) plus CTX, and SLBZP (M) plus CTX groups were; 52.39%, 45.84%, 58.41% and 52.77% respectively. Compared to the model; group, the protein expressions of PDGF-BB, Ang-1, Ang-2, and XIAP in; tumor tissue were obviously lower than those in all treated groups. And; the expressions of Caspase-3 and Caspase-9 in tumor tissue showed a; trend of up-regulation. And the effect in SLBZP (H) plus CTX group was; the most significant (P<0.01, P<0.05). Conclusion: SLBZP can more; effectively adjust the expression of apoptosis related proteins of tumor; in mice with H22 hepatocellular carcinoma cells, and promoting tumor; apoptosis may be one of the possible mechanisms of SLBZP to improve; chemotherapy in treating hepatic cancer.国家自然科学青年基金项目; 福建省自然科学基金项目; 福建省大学生创新创业训练项

Study on Physiological Indexes of Typical Species in the Lower Reaches of Tarim River --Populus euphratica as example

自2000 年塔里木河下游生态输水工程实施以来，塔里木河下游以胡杨(P.euphratica)为主要建群种的天然植被发生了极大变化。本文通过研究不同长势胡杨以及胡杨树冠不同部位上的叶片生理指标变化规律，来反映其生态变化，为将生态特征和生理特性相结合解释输水后植被变化的原因提供理论依据，同时，这也对塔里木河下游生态系统恢复有重要意义。本研究将不同生长状况的胡杨划分成5 个长势等级(VS1~5)，并将胡杨树冠分成上、中、下三层(VS1~3)，测定其叶片相对含水量(RWC)、叶绿素(Chl)、丙二醛(MDA)、可溶性糖(SS)、脯氨酸(Pro)含量及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性，结合实验室聚乙二醇(PEG)-6000 干旱胁迫模拟和NaCl 盐分胁迫模拟条件下胀果甘草lycyrrhiza inflata)和疏叶骆驼刺(Alhagi sparsifolia)的MDA、SS 和Pro含量及SOD 和POD 活性的变化规律，用最小显著差数(LSD)检验和Pearson 相关分析等方法研究胡杨不同长势等级及不同冠层生理特征，然后进一步分析主要生理指标与地下水埋深和胡杨树干径向生长量的关系。得出以下主要结论：(1)胡杨长势逐级衰败，不同生理指标表现出不同的变化规律：叶片RWC 递减、MDA 含量递增，而叶片Chl、SS 和Pro 的含量及SOD 和POD 活性均表现出先增后减的变化趋势。这些结果表明：植物在受到胁迫时生理调节是在一定范围内实现的，当胁迫最强时，调节机能反而下降。(2)胡杨不同树冠层次上的主要生理指标变化规律亦不同，主要生理指标中，只有Pro 含量及SOD 和POD 的活性在胡杨不同冠层上有显著差异(P&lt;0.05)，而目前的研究还不足以解释其枯梢、枯顶现象的原因，但是可以部分解释树冠不同部位所受胁迫存在差异。(3)从本文的研究结果看，单纯某个生理指标的高低并不足以反映胡杨所遭受的环境胁迫以及环境胁迫的程度，生理指标的变化需要与生态变化、环境条件协同分析

«Диалог как средство формирования коммуникативной компетенции у студентов, изучающих русский язык как иностранный (с опорой на статью Л.Выготского «Мышление и речь»)

[[abstract]]В данной статье рассматривается диалог как средство формирования коммуникативной компетенции у студентов, изучающих русский язык как иностранный. По Выготскому мысль не состоит из отдельных слов; «То, что в мысли содержится симультанно, в речи развертывается сукцессивно. Мысль можно было бы сравнить с нависшим облаком, которое проливается дождем слов. Поэтому процесс перехода от мысли к речи представляет собой чрезвычайно сложный процесс расчленения мысли и ее воссоздания в словах». Этому переходу от мысли к речи, особенно на начальном этапе, и помогает обучение на основе диалога, который, как считают многие лингвисты, является самой естественной формой речи. Изучив технологию обучения и систему упражнений диалогической речи, мы пришли к выводу, что обучение диалогической речи с помощью серии упражнений предполагает овладение навыками и умениями, нужными для реализации ситуации общения в соответствии с коммуникативными задачами обучающихся. Students of Russian as a Foreign Language will benefit from a pedagogical method that capitalizes on dialogue; it shapes their communicative competence. Lev Vygotsky (1896 - 1934) wrote that "thought, unlike speech, does not consist of separate units. A thought may be compared to a cloud shedding a shower of words. Precisely because thought does not automatically equate with words, the transition from thought to word is through meaning. Because a direct transition from thought to word is impossible, people have always lament about the inexpressibility of thought". So, the training through the dialogues (some linguists consider dialogue the natural form of oral speech, the one in which language fully reveals its nature), is necessary. Special grammar exercises, especially at the beginning stage of learning, helps the smooth transition from thought to speech, helps to get some skills to realize communication in accordance with the student's tasks.[[notice]]補正完畢[[journaltype]]國內[[ispeerreviewed]]Y[[booktype]]紙本[[countrycodes]]TW

[[alternative]]包里斯‧里日詩作中的價值規範

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Слова - композиты новейшнего периода и их отражение в словарях

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Рост анализтизмов в современном русском языке

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Lexical and grammatical innovations in modern Russian

[[abstract]]This article is devoted to the analysis of attributive groups in the modern Russian language and their functioning in the Internet discourse. The author offers different classifications of compound words.[[notice]]補正完