Marketing strategy of Guizhou Power grid

2002年底我国电力体制改革后，电网企业作为市场主体独立运作。电网的自然垄断和基础性行业的特殊地位，使其市场营销模式也独具特色。伴随着社会经济的不断发展，电网企业总是处在供需矛盾的不平衡之中，但它们都很好的完成了自己的使命。 贵州电网公司是一家省级供电企业，它在负责完成本省范围内的供电任务的同时还担负着“西电东送”重任，所以在这其中的矛盾和问题会显得更多也更特殊。随着“十二五”规划的全面落实，针对这些问题和矛盾应该有所思考，同时还应该寻求解决之道，这就是本文想做的事，如能给同行以借鉴应属无心插柳之举。 本文共分六章，对贵州电网电力市场营销问题进行分析并探求解决的思路和方法。 第一章是绪论...The power grid enterprises become main players in the market and run independently and operate when our country made the reform of electric power system after 2002. Power grid was belong to natural monopoly and special position foundation industry, cause it’s marketing model that have some peculiar characteristic. With the development of society and economy, power grid enterprises always in imbala...学位：工商管理硕士院系专业：管理学院高级经理教育中心（EMBA项目）_高级管理人员工商管理硕士(EMBA)学号：X201015601

Effect and mechanism of TIPE3 interference plasmid on SW480 colorectal cancer growth

目的:通过TIPE3干扰质粒转染SW480结肠癌细胞,验证干扰TIPE3表达对SW480结肠癌细胞生长的影响并探讨相关机制。方法:构建TIPE3; -shRNA-pSIREN-RetroQ干扰质粒,通过脂质体转染法成功将干扰质粒导入SW480细胞,通过RT-PCR、Western; blot检测重组质粒的干扰效率。应用CCK-8方法检测SW480细胞生存率。AnnexinV-FITC/PI流式细胞法检测细胞凋亡。使用West; ern; blot检测细胞增殖、凋亡相关分子的表达情况。结果:成功设计、构建和筛选具有生物活性且干扰效率最佳的TIPE3-shRNA-pSIREN-Ret; roQ干扰质粒。CCK-8检测证实干扰SW480结肠癌细胞TIPE3表达可以抑制细胞生长。流式结果显示,TIPE3-shRNA3干扰组的凋亡率为; (27. 991. 087) %,显著高于正常细胞组(12. 102. 213) %及转染空载质粒组(11. 440. 277 0); %。证实了降低TIPE3表达可以增加SW480对aDR5ScFv所诱导的细胞凋亡敏感性。Western; blot结果显示干扰TIPE3表达可以活化caspase3蛋白,降低p-AKT、p-PDK1、PCNA等分子的表达。结论:干扰TIPE3的表达对; SW480结肠癌细胞具有促进凋亡,抑制增殖的作用。Objective: To study the effect of interference TIPE3 on the colon cancer; cell growth by transfecting SW480 colon cancer cells with the TIPE3; interference plasmid were detected. Methods: Transfecting the; constructed TIPE3-shRNA-pSIREN-RetroQ plasmid to SW480 cells. To; determine the highest interference efficiency plasmid, the mRNA and; protein levels of recombined plasmid were detected by RT-PCR and Western; blot separately and tested the cell proliferation with CCK8. Meanwhile,; apoptosis rate of SW480 cells was determined by flow cytometry assay; with AnnexinV-FITC/PI. To further determined the effects of recombined; plasmid on cell development, the level of protein involved in; proliferation and apoptosis were detected by Western blot. Results: The; most effecient interference plasmids were successfully constructed. We; found that the cell survival rate decreased when interference TIPE3 gene; expressing in colorectal cancer cells. Flow cytometry indicated that; interefering the expression of TIPE3 would increase the sensitivity of; SW480 cell to apoptosis induced by aDR5ScFv. The results of Western blot; showed that low expression of TIPE3 would activate caspase3 and; downregulate the expression of p-AKT,p-PDK1 and PCNA. Conclusion:; Interference TIPE3 could promote apoptosis and inhibit proliferation in; SW480 colon cancer cells.国家自然科学基金; 福建省属公益类科研院所基本科研专项项

Construction and identification of interference plasmid targeting on TNFAIP8

目的:构建并筛选出干扰效率最佳的TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒。方法:通过生物软件选择3个TnfAIP8基因干扰位点,构建干扰质粒并测序验证,将干扰质粒及对照质粒分别转染至A549细胞,通过rT-PCr、WESTErn blOT检测干扰效率。结果:经rT-PCr和WESTErn-blOT证实TnfAIP8-SHrnA-P SIrEn-rETrO Q干扰质粒能有效干扰并抑制细胞内TnfAIP8基因的表达,通过流式检测发现降低TnVAIP8表达可以提高细胞对A dr5SC fV诱导凋亡的敏感性。结论:成功构建和设计了对TnfAIP8基因具有显著干扰效率的干扰质粒,为进一步研究TnfAIP8基因的功能奠定了基础。Objective: To construct and screen the high efficiency interference plasmid of TFAIP8-shRNA-p SIRENRetro Q.Methods: Selected and synthesized three Target Sequence of TNFAIP8 shRNA1,TNFAIP8 shRNA2,TNFAIP8 shRNA3,and construct the TNFAIP8 interference plasmid.Transfection TNFAIP8-shRNA-p SIREN-Retro Q interference plasmid to A549 cells.Filter out the highest interference efficiency plasmid by detecting the mRNA and protein levels using RT-PCR and Western blot methods.Results: We successfully design and built three TNFAIP8-shRNA-p SIREN-Retro Q interference plasmids,and screen out the highest efficiency interference plasmid.Conclusion: Three interference plasmids targeting the TNFAIP8 gene have been constructed successfully and provide a useful tool for studying the function of TNFAIP8.国家自然科学基金项目(81272720); 福建省卫计委医学创新课题(2014-CXB-43); 厦门市科技计划项目(3502Z2083008)资

The Inner Communication Bus of AUV

设计了基于CAN协议的AUV内部通讯总线系统 .系统通过协议转换器的模块化、可配置性设计满足AUV系统对其内部通讯总线的开放性要求 .协议转换器内部的容错处理能力以及紧急事件处理节点的设计为增强AUV系统的可靠性和容错能力、为避免AUV在深海工作环境下丢失增加有力的保障措

Dams Construction and Agricultural Development of Dams During Converting Slope Cropland to Woodland and Grassland

以方塔流域为实例,讨论了退耕还林还草下的坝系及坝系农业发展问题,笔者认为淤地坝与退耕还林还草工程有机结合,构成了流域阻止泥沙出沟入黄的稳固屏障。建议在对流域生态经济问题调研的基础上,分析淤地坝建设及坝系农业发展的方向和规模

应变补偿InGaAs/InAlAs量子级联激光器

利用应变补偿的方法研制出激射波长λ≈3.5-3.7μm的量子经联激光器。条宽20μm,腔长1.6mm的In_xGa_(1x)As/In_yAl_(1-y)As量子级联激光器已实现室温准连续激射。在最大输出功率处的准连续激射可持续30mn以上

苯在铂电极上溴代反应的共焦显微拉曼光谱研究

本文主要利用共焦显微拉曼技术检测KBr溶液中苯在光滑铂电极上的取代反应。结果表明 ,苯在 1 2V时将和溴离子在氧化产生的溴自由基发生溴代反应 ,与氯代反应相比 ,溴代反应更剧烈、产物更复杂、更容易受光照影