Study on Natural Products from Five Edible and Medicinal Macrofungi

天然小分子化合物种类繁多、数量巨大，不但具有化学调节、信号载体、化学防御等多种生理作用，而且是药物开发的重要资源。组合生物合成等技术可以定向合成与创造新的天然产物衍生物，但作为药物发现的工具还有待进一步发展和完善。天然产物的多样性是任何科学家无法想象的，仍然是新药研发的重要先导化合物。食药用大型真菌是产生新结构与具有活性天然小分子化合物的重要生物类群。近年来，越来越多的食药用真菌可以通过液体发酵或固体发酵方式来大量培养，而且它们发酵产物的营养价值与化学成分含量远高于天然或人工栽培的子实体或菌核。因此，研究食药用真菌发酵产生的天然小分子化合物的多样性与活性已成为天然产物化学家与真菌学家共同关注的...Large quantity of natural products with different structure types have not only been focused on the study of functional molecules for the organism in the fields of chemical defense, chemical regulation and signal transduction, but also played a vital role in the discovery of new pharmacy. Many natural product derivatives with specific structure can be produced through combinatorial chemistry and o...学位：理学博士院系专业：生命科学学院生物化学与生物技术系_微生物学学号：2012005140313

Clavicoronol,a new metabolite,from the mycelia culture of Clavicorona pyxidata

目的对珊瑚菌(ClAVICOrOnA PyXIdATA)菌体培养物的活性成分进行分离。方法用多种色谱技术对化合物进行分离纯化,并用光谱技术和单晶X射线衍射技术鉴定化合物的结构。结果从中分离到2个化合物:ClAVICOrOnOl(1)、腺苷(2)。结论化合物1为新化合物,并通过单晶X射线衍射技术确定了2的立体构型。Aim To explore the new bioactive metabolites from the mycelia culture of the edible mushroom Clavicorona pyxidata.Methods The constituents were isolated and purified by column chromatography,and their structures were identified by spectroscopic analyses,including 1D-and 2D-NMR data,and single-crystal X-ray diffraction.Results A new natural product,named clavicoronol(1) along with adenosine(2),were obtained.Conclusion Compound 1 is a new compound and stereochemistry of 2 was confirmed by a single-crystal X-ray diffraction.ThisworkwasfinanciallysupportedbytheNationalNaturalScienceFoundationofChina(30500632);theNationalScienceFundforDistinguishedYoungScholarstoY.-M.Shen(30325044

Isolation, Structure Elucidation and Apoptosis-inducing Activity of New Compounds from the Edible Fungus Lentinus striguellus

Three new natural compounds, striguellolide A (1), striguellol A (2) and striguellone A (6), together with three known ones, 3,4-trans-dihydroxy-6-methoxy-2,2-dimethylchroman (3), 3-hydroxy-6-methoxy-2,2-dimethylchroman-4-one (4) and 3-hydroxy-6- methoxy-2,2-dimethylchroman (5), were isolated from the agar cultures of the edible fungal strain Lentinus striguellus. Their structures were elucidated by spectroscopic and mass spectrometric analyses, including 1D-, 2D-NMR and HR-ESI-Q-TOF-MS, and chemical reactions. Striguellone A showed cytotoxicity against HeLa cells by MTT assay and was found to be an activator of apoptosis, assessed by morphological observation and cell cycle analysis using the flow cytometer