Separation and purification of astaxanthin from Phaffia rhodozyma and the immunomechanism of astaxanthin

虾青素是一种具有极强抗氧化和抗衰老活性的类胡萝卜素，被誉为“超级维生素E”，在医药、保健和化妆品等方面有着广泛的应用价值。为了满足日益增加的市场需求，急需进一步降低虾青素的生产成本，目前市售虾青素主要来源是雨生红球藻，而对于红发夫酵母来源虾青素的分离提纯却鲜有报道；另一方面，虽然虾青素强大的生理功能已逐渐被人所知，但对其免疫功能及其作用机理尚未有深入的研究报道，为使虾青素在人类健康方面更好地发挥作用，对其免疫机理的研究也是非常必要的。 本文首先对红发夫酵母虾青素的分离纯化进行研究。采用高压匀浆破碎法破碎红发夫酵母细胞，考察不同破碎压力、破碎次数和处理量对细胞破壁及虾青素萃取率的影响；结果表明...Astaxanthin, one kind of high-effective antioxidative and antiaging carotenoid, is well-known as "super vitamin E"and widely utilized in pharmaceuticals, cosmetics and health care fields. In order to meet the increasing market demand, it is very urgent to reduce the cost of astaxanthin production. Haematococcus pluvialis is the main soure of commercial astaxanthin currently. However, no special at...学位：工学硕士院系专业：化学化工学院_化学工程学号：2062010115143

Effect of Astaxanthin on Immunological Indexes of Normal Mice and Aging Model Rats

虾青素是一种具有极强抗氧化和抗衰老活性的类胡萝卜素,在医药、食品和化妆品等方面有着广泛的应用价值.以小鼠和大鼠为研究对象,考察虾青素对生物体免疫性能的影响.通过对小鼠灌胃低(4Mg/(kg·d))、中(20Mg/(kg·d))、高剂量(100Mg/(kg·d))的虾青素,考察实验组与对照组的免疫器官脏器指数、巨噬细胞吞噬功能和血清抗体水平.结果表明,各剂量组小鼠血清抗体水平均显著升高(P<0.01),中剂量组小鼠脾脏指数显著升高(P<0.05),中、高剂量组小鼠的胸腺指数和吞噬系数均显著升高(P<0.05).采用腹腔注射d-半乳糖的方式建立大鼠的致衰老模型,考察虾青素灌胃对其免疫器官脏器指数及其形态的影响.结果显示,衰老灌胃虾青素组与衰老对照组相比胸腺指数显著升高(P<0.01),同时d-半乳糖诱导致使的肝脏、胸腺和脾脏细胞损伤得到明显改善.由此可以看出,虾青素可提高非特异性免疫和体液免疫功能,该结果为虾青素免疫机理的进一步研究奠定了基础.Astaxanthin,a high-effective antioxidative and antiaging carotenoid,is widely utilized on pharmaceuticals and health care fields.To evaluate the effect of astaxanthin on immune function,experiments in mice and rats were conducted.The immune organs indexes,macrophage phagocytic function and serum antibody level of mice were studied through different dose gavage(low dose=4mg/(kg·d),mid dose=20mg/(kg·d),and high dose=100mg/(kg·d).Compared with the control group,the antibody level in all treated groups(p<0.01),the spleen indexes in mid-dosage group(p<0.05),the thymus indexes and the phagocytosis indexes in both mid and high-dosage group(p<0.05)were significantly increased.The aging rats were made by injecting D-gal into abdominal cavity continually,then the empact of lavaging astaxanthin on the immune organ indexes and organ morphology were observed.The results showed that the thymus index of aging model rats was significantly raised(p<0.01)by gavaging astaxanthin,and the cell injury of liver,thymus and spleen induced by D-galactose was obviously improved.This study demonstrated astaxanthin can enhance nonspecific immunity and humoral immune function,which laid a foundation for study on immune mechanism of astaxanthin.国家自然科学基金项目(31071488); 国家海洋公益性行业项目(201205020); 中央高校基本科研业务费专项资金(20101201049