354 research outputs found
Secondary Functions And Novel Inhibitors Of Aminoacyl-Trna Synthetases
Get PDFThe aminoacyl-tRNA synthetases are a family of enzymes involved in the process of translation, more specifically, ligating amino acids to their cognate tRNA molecules. Recent evidence suggests that aminoacyl-tRNA synthetases are capable of aminoacylating proteins, some of which are involved in the autophagy pathway. Here, we test the conditions under which E. coli and human threonyl-tRNA synthetases, as well as hisidyl-tRNA synthetase aminoacylate themselves. These reactions are ATP dependent, stimulated by Mg2+, and are inhibited by increasing cognate tRNA concentrations. These data represent the foundation for future aminoacylation experiments, specifically delving into the relationship between the autophagy pathway and the aminoacylation of proteins.
Additionally, we provide evidence of the inhibitory abilities of the compound EHTS-0 on both E. coli and human threonyl-tRNA synthetases. Further, we also show that an EHTS-0 analog, EHTS-1, also significantly inhibits E. coli threonyl-tRNA synthetase but not the human enzyme. These data could be useful in determining the potential for EHTS-0 and EHTS-1 as possibly anti-angiogenic drugs
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A Democracy of Knowledge
Get PDFI am very honored to speak to this group today. l owe an enormous debt to the work that you do and I very sincerely thank you for it. Documentary editing has, for me, an almost priestly connotation. I went to a Jesuit high school where my toughest teacher was Fr. Alphonse Yumont, who taught Latin. Every night we had to pore over a long section of Vergil, and the next morning had to translate it aloud in class. Errors were not allowed. And by errors I mean any deviations whatever from a precisely literal translation. We were not allowed to glide over obscurities or embellish with flights of fancy. I can still hear the slap of Fr. Yumont\u27s stick on the desk as he shrieked, Mister! Look at the text! Don\u27t tell me what you think it means. Tell me what it says!
From Fr. Yumont I acquired a habit of paying meticulous, maniacal attention to the text -the holy object right in front of you. From him I learned that if you paid very close attention to the text you would learn things richer and more complex than the interpretations created by your imagination. If you refused to gloss over the obscurities and the ambiguities, but struggled instead to comprehend a text exactly as it came down to you, you would enter the mind and the distant world of someone else from long ago. And if your translation turned out to be a bit duller than you wished, at least it had the advantage of being true
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