The effect of oregano (Origanum vulgare) extract on prevention of S. ineae experimental infection in rainbow trout (Oncorhynchus mykiss)

This study was performed in order to determine the effect of Oregano (Origanum vulgare) extract on Streptococcus ineae infection in rainbow trout. 063 rainbow trout weighing 15±1 gr were randomly allocated into three treatment groups with three repetition, including: 1) Control group, 2) Oregano extract treated group (1% of diet), 3) Felorfenicol treated group (10mg/kg fish), all feed one time a day for two weeks. At the end of the second week, 5 fish of each repetition collected and sampled from their blood and tissue of spleen and kidney. After the bleeding, all the samples injected with streptococcus ineae. After two weeks mortality rate calculated and after the bleeding, sampled from the spleen and kidney of the samples. Total cunt analysis conducted on the spleen samples in 4 logarithmic concentrations. in the second phase all the samples primarily injected with S.ineae and then treated with Oregano concentration and Felorfenicol for two weeks. After the two steps of bleeding, in sterile qualifications, the spleen of the fishes sampled for total cunt analysis. Blood cells counting and lisosym activity indicated in all blood samples. The results shown that total mortality in both of the phases of experiment in Oregano extract (OE) treatment was significantly lower comparing to control group (p<0.05). Furthermore the results of blood cells counting shown that WBC and monocyts percents were significantly higher in Oe and Felorfenicol treatment groups (p<0.05). This phenomenon was because of the effects of Oe and Felorfenicol. Nevertheless the lysozym activity degreased comparing to control group (p<0.05). But total count analysis shown that bacterial audience in spleen degreased treating Oe and felorfenicol. It was concluded that supplementation of Oe registered lower mortality and bacterial audience, and better hematological parameters in fishes, compared with control group. Therefore supplementation of Oe in fish diet would be effective in prevention and treatment of S.iniae in rainbow trout

Analysis of vitellogenin gene structure in Caspian roach, Rutilus caspicus (Pisces: Cyprinidae) during exposure to Atrazine

Chemical contamination of aquatic environments to EDCs has become a major focus of environmental toxicology research. The exposure of fishes to estrogenic EDCs in aquatic environments is most frequently assessed by analyzing Vitellogenin (Vg) (the egg yolk precursor protein) expression. Therefore, characterization of Vg gene is of high priority for EDCs bio-monitoring. So, we prepared liver tissue samples of Caspian roach, Rutilus caspicus for RNA extraction. Following the cDNA synthesis, specifically - designed primers were employed to amplify the Vg gene and ultimately sequence it. The evolutionary analyses of the sequence were performed using MEGA7 software. The obtained results indicated that the designed primers successfully amplified the partial cDNA sequence. Our results indicated that this sequence most probably belongs to the Vg1 form of the gene. Moreover, it was demonstrated that Caspian roach and Petroleuciscus esfahani share a common ancestor. Noteworthy, the study of Vg gene would be helpful to understand the molecular mechanisms of development and would be used to establish a bio-monitoring tool for detection of exposure to different EDCs

The Prevalence of Musculoskeletal Disorders and its Predictors among Iranians’ Housewives

ABSTRACT Background and aims: Musculoskeletal disorders (MSDs) are common among housewives. Therefore, this study aimed to estimate the prevalence of MSDs and its predictors among a group of Iranian housewives. Methods: This cross-sectional study carried out on 600 Iranian housewives in 2014 that were selected using multistage random sampling from three cities including Karaj, Kashan and Yazd. The data were collected using Nordic questionnaire and were analyzed in SPSS software using chi-square and multiple linear regressions with Hosmer-Lemshow method. Results: The total prevalence of MSDs was 53 and was not different in three cities (P>0.05). The highest prevalence of pain was found to be in the back region (51.33), followed by neck (51) and shoulder (41.5) and least at wrist (40.5). The possibility of pain in neck was related to husband’s job (governmental compared to self-employed OR=1.6, CI 95:1.09-2.63), older age (OR=1.06, CI 95:1.01-1.11), higher height (OR=1.09, CI 95:1.03-2.09), and more marriage duration (OR=1.08, CI 95:1.01-2.05). The possibility of pain in back was related to educational level guidance (OR=0.38, CI 95%:0.15-0.95) and high school (OR=0.31, CI 95%:0.13-0.94) compared to primary school and heavier weight (OR=1.07, CI 95%:1.04-2.19), and finally the possibility of pain in shoulder was related to dominant hand right hand compared to both hand (OR=4.6, CI 95%:1.2-17.8). Conclusion: Present study showed that the prevalence of MSDs in Iranian housewives is high and the educational level, height, weight and having husband with governmental job are as important predictors of MSDs among housewives. However, ergonomic training and informing the housewives about the risk factors of MSDs could prevent and postponed the occurrence of these disorders

The prevalence of dental caries in primary molars and its related factors in 6 and 7 years old children in Shemiranat health center

Background and Aims: Healthy People plan that is expressed by WHO stipulated that by 2000 at least 50% of the children and by 2010, 90% of 6-5 year-old children should be with no caries in their primary teeth. The present study investigated the prevalence of teeth decay of D, E and related factors in 6 and 7 years old children.Material and Methods: This cross-sectional study was conducted in 2013 to assess the bases covered by student Shemiranat health center. Study population including 5000 students aged 6 and 7 and the sample size of 450 patients was available in sampling method. Data in a form that was designed for this study were collected and analyzed using Epi data and SPSS software.Results: The index dmft (Decayed Missed Filled Teeth) in primary teeth D and E is equaled to 2.46 and prevalence of dental caries E and D were estimated 63.4%. There is significant relationship between decay of teeth D and E and father education (Pvalue&lt;0.001) and mother education (Pvalue&lt;0.001) children age (Pvalue&lt;0.001) locality of children whether they live in rural or urban regions (Pvalue &lt;0.001) economic condition of families (P value&lt;0.001) and social status (Pvalue&lt;0.001) with number of decay teeth.Conclusion: The results imply on high prevalence of teeth caries among the children molar covered by Shemiranat health center so that the number of teeth without caries is far from the global measures and WHO. Likewise there is a significant relationship between social and economical status with children decay teeth and there is a need to pay more attention to mouth health in poor regions from the social and economical point of views.Key words: Prevalence of carries, D and E teeth, dm

Survey on alimentary tract parasites in cultured fish in Khouzestan province

In this study which was carried out in khoozestan province, 1100 pieces of common carp (Cyprinus carpio), silver carp (Hypophthalmichthys molitrix), Grass carp (Ctenopharyngodon idella) and Big head (Arysthychthys nobilis) from different parts of the province were examined. From each part, 10% of the fish farms were sampled according to the fish density at each farm. parasitic infection of gastrointestinal tract were studied on 600 pieces of silver carp (60% ) , 244 pieces of common carp (25%) , 51 pieces of Big head (5%) . only Grass carp was infested to protozoan and metazoan parasites . thirty percent of examined Grass carp of 2-730 gr weight were infested to Hexamita but infestation was more severe in Grass carp with 20-40 gr weight . macroscopic manifestation included : dysphagia , weakness , circulating movements, paling of gills , stomach inflammation , intestinal inflammation , ascitis and yellowish gut contents . in severe and intestinal necrosis along with hyperemia was apparent . the cestoda , bothriocephalus opsariichthydis ( Rudolphi , 1808 ) with an infestation rate of 35 % were only observed in Grass carp in 2-40 gr . Grass carp that showed severe infestation, intestinal blockade was observed with more than 100 parasites. The length of adult cestoda was 450-530 mm and its width was 5mm. in severe infestations, macroscopic manifestations included: ascitis, abdominal inflammation, swimming on water surface and emaciation. Histologic studies showed necrosis of intestinal wall, hemorrhage and localized necrosis. In fishes with severe infestations, inflammation was observed. Infestation with the nematode, capilaria was observed in 10% of 20-750 gr Grass carp. No macroscopic symptoms were identified in the samples, but microscopic examination showed acute necrosis along with hyperamia and submocusal hemorrhage. Lymphocytes were also observed. Adult nematode had a width of 0.075 and a length of 7.5-8.5 mm

Standardized patients versus simulated patients in medical education: are they the same or different

In order to equip medical students with all the necessary skills in dealing with patients to provide optimal treatment, the need for the use of real patients in educational settings has become prominent. But all the required skills cannot be practiced on real patients due to patients’ safety and well-being. Thus, the use of standardized patients (SPs) or simulated patients (SiPs) as a substitute for real patients signifies their importance in simulationbased medical education. One question raised in regard to using SPs or SiPs in order to enhance medical students’ tangible and intangible skills in a safe controlled environment is whether these two terminologies are the same or different? Various studies use these terms interchangeably and do not consider a difference between them. Based on our literature review, there seems to be differences between these two modalities. We also try to highlight the advantages of these modalities in clinical encounters

Bibliometric and Content Analysis of Scientific Outputs Relevant to Health Education and Promotion in Iran during 1998-2011

Background: Since the aim of health research is to improve the quality of community life, the results obtained from these research could be used in health policies and practices. Therefore, the aim of this study was to evaluate the bibliometric and content analysis of research relevant to health education and promotion performed in Iran during 1998-2011. Methods: This is a descriptive content analysis study. Data were collected from online databases (IranMedex, Medlib, SID, ISI, and PubMed), and all published articles relevant to health education and promotion in Iran up to the end of March 2011 were included in this study. In order to do content analysis a researcher-made checklist approved by experts was used for identifying the variables. Data was entered into SPSS 21 and descriptive statistics and percents were calculated. Results: From 1227 documents retrieved, 817 were published in Persian and 410 in English. A great share of retrieved documents belonged to the articles published in the scientific journals (87.4%). Mental health (10.7%), family planning and reproductive health (10.2 %), and chronic non-communicable diseases (10.2%) were the most frequent subject areas in research relevant to health promotion, respectively. Most of the studies were cross-sectional (52.1%), the most widely used method was simple random sampling (27.7%), and most of the studies were performed locally in provinces (83.7%). Conclusion: Doing more studies by outlining the exact perspective of health promotion research in Iran and comparison of the current status of Iran with that of other countries are recommended

Analysis of vitellogenin gene structure in Caspian roach, Rutilus caspicus (Pisces: Cyprinidae) during exposure to Atrazine

Chemical contamination of aquatic environments to EDCs has become a major focus of environmental toxicology research. The exposure of fishes to estrogenic EDCs in aquatic environments is most frequently assessed by analyzing vitellogenin (Vg) (the egg yolk precursor protein) expression. Therefore, characterization of Vg gene is of high priority for EDCs bio-monitoring. So, we prepared liver tissue samples of Caspian roach, Rutilus caspicus for RNA extraction. Following the cDNA synthesis, specifically-designed primers were employed to amplify the Vg gene and ultimately sequence it. The evolutionary analyses of the sequence were performed using MEGA7 software. The obtained results indicated that the designed primers successfully amplified the partial cDNA sequence. Our results indicated that this sequence most probably belongs to the Vg1 form of the gene. Moreover, it was demonstrated that Caspian roach and Petroleuciscus esfahani share a common ancestor. Noteworthy, the study of Vg gene would be helpful to understand the molecular mechanisms of development and would be used to establish a bio-monitoring tool for detection of exposure to different EDCs

A quantitative literature-curated gold standard for kinase-substrate pairs

We describe the Yeast Kinase Interaction Database (KID, http://www.moseslab.csb.utoronto.ca/KID/), which contains high- and low-throughput data relevant to phosphorylation events. KID includes 6,225 low-throughput and 21,990 high-throughput interactions, from greater than 35,000 experiments. By quantitatively integrating these data, we identified 517 high-confidence kinase-substrate pairs that we consider a gold standard. We show that this gold standard can be used to assess published high-throughput datasets, suggesting that it will enable similar rigorous assessments in the future

Design, Implementation, and Evaluation of a Short-Term Research Skills Training Course for Clinical Faculty Members: A New Experience at Kerman University of Medical Sciences

Background: Several research skills training courses are designed for both faculty members and students in educational and research institutions around the world. Objectives: The current study aimed to design, implement, and evaluate a short-term research skills training course for faculty members of Kerman University of Medical Sciences. Methods: The current scholarship study was conducted in three stages, in 2017. The first stage comprised of a comparative study needs assessment, and course design. The training course was conducted with the participation of 30 faculty members and the teaching of 10 experienced professors. Participants were evaluated through practical work and homework. Instructors were evaluated by participants using survey forms. The course was evaluated through interviews with several participants and professors. Results: After eight months of research and holding 16 sessions (which each took between 2 to 4 hours), a course with three main modules was designed in 2017. Instructors used appropriate teaching methods, including interaction with participants, problem-solving, discussing, teamwork, and practicing at home. According to the results of the evaluation, the content of the course was appropriate for the research activities of participants. Besides, they believed that the instructors were among the strengths of the course. Conclusion: Certainly, increasing research skills of faculty members will lead to better guidance of assistants and qualitative improvement of research conducted by students. Keywords: Training; Research skills; Faculty; Medica