Back to soil: Awakening the production of cryptic antibiotics in Streptomyces

Most of the antibiotic biosynthetic gene clusters (BGCs) in Streptomyces are not expressed under laboratory conditions, however these clusters are maintained in the genomes, therefore indicating that they must play important roles in adaptation and survival within their ecological niches. Understanding the global regulation patterns that affect transcription of antibiotic BGCs in soil conditions versus laboratory conditions allows us to identify novel routes for up- or down- regulation in order to trigger expression of these BGCs and therefore production of these antibiotics under controlled laboratory and fermentation conditions. Cryptic pathways represent an untapped resource in terms of new metabolites that could be very useful in the clinic if we can awaken their expression and production. We are using RNA sequencing to analyse global transcriptional patterns of S. venezuelae and S. rimosus grown in sterile soil, non-sterile soil and standard laboratory solid medium, as well as using an unbiased metabolomics approach to identify compounds produced in soils. These results will enable the identification of global regulatory pathways that activate cryptic clusters in nature and lead to the production of these compounds in soil. This will enable us to generate genetically modified strains engineered to increase the yield of both well characterised and cryptic clusters which encode potentially novel compounds under industrial fermentation conditions. It is worth noting that the transcriptional data may also help shed light on the complex regulation of the life cycle of these Streptomyces strains in an ecologically relevant environment

Encouraging the Productivity of Female Millennial Employees Who Apply Knowledge to Enhance Products and Services

The almost 20 million female millennial knowledge workers enhance products, services, and customers in American businesses by applying their skillset knowledge. While participative management theories, like William Ouchi's Theory Z, are known to promote employee productivity and loyalty it is noted that they only focus on the majority population. This study looked to extend existing participative management theories to include the female millennial knowledge worker and avoid negative costs associated with poor productivity and turnover. This manuscript highlights key results from a qualitative embedded single-case study that explored the influence participative management techniques had on the turnover and productivity of female millennial knowledge workers. This study extended participative management theory to include the female millennial knowledge worker and noted that managers with female millennial knowledge employees should consider a focus on employee development, timely feedback, and cultivating a positive working environment. Keywords: female, millennial, knowledge worker, participative management, productivity, turnover.

BDES1020 Continuous City <Paros, Huckstepp>

New York is home to fashion, music, film, art, business… and thousands of homeless people. The proposed design aims to physically get these people “off the streets”. The building clings to the Radio City Music Hall. Below, a new street space has been carved into the block. It has been filled with a new kitchen, clothing depot, and multi-purpose levels to provide space to the public. It will become an area to share, develop, and grow with those less fortunate. The shelter itself consists of individual bedroom pods and a bathroom on each floor. These pods designed to provide a private space for an individual. By providing each room with a louver system that allows the occupants to control their level of privacy and views

BDES1020 Continuous City <Paros, Huckstepp>

New York is home to fashion, music, film, art, business… and thousands of homeless people. The proposed design aims to physically get these people “off the streets”. The building clings to the Radio City Music Hall. Below, a new street space has been carved into the block. It has been filled with a new kitchen, clothing depot, and multi-purpose levels to provide space to the public. It will become an area to share, develop, and grow with those less fortunate. The shelter itself consists of individual bedroom pods and a bathroom on each floor. These pods designed to provide a private space for an individual. By providing each room with a louver system that allows the occupants to control their level of privacy and views