Multi-micronutrient supplementation in HIV-infected South African children : effect on nutritional s tatus, diarrhoea and respiratory infections

Background: The nutritional status of HIV-infected children is reported to be poor. Diarrhoea and acute respiratory infections tend to be more common and severe in HIV-infected children than in uninfected ones. Deficiencies of micronutrients may result in poor growth and increased risk of diarrhoea and respiratory infections. Micronutrient deficiencies are common in HIV-infected children. The poor growth, diarrhoea and respiratory infections seen in HIV-infected children may be partly due to micronutrient deficiencies. The studies in this thesis had two main objectives: (1) to evaluate the effect of short-term (during hospitalization) and long-term (6 months) multi-micronutrient supplementation on episodes of diarrhoea and respiratory infections in HIV-infected children who are not yet on antiretroviral therapy (ART), and (2) to assess the effects of long-term multi-micronutrient supplementation on appetite and growth performance of HIV-infected who are not on ART. Methods and results: Four studies were conducted. Initially a cross-sectional study was performed in which the duration of hospitalization, weight, length, micronutrient status and appetite of HIV-infected children admitted with diarrhoea or pneumonia was compared with the results of HIV-uninfected children. Duration of hospitalization was 2.8 days (52%) longer in HIV-infected children. Appetite as measured by amount of test food eaten (g per kg body weight) was 26% poorer in HIV-infected children. Mean length-for-age Z-scores were lower in HIV-infected children; there was no difference in level of wasting. Subsequently multi-micronutrient supplementation studies were performed, one short-term and two long-term studies. The effect of supplementation on the duration of hospitalization in HIV-infected children with diarrhoea or pneumonia was assessed in the short-term study. One long-term study assessed the supplement’s impact on growth and frequency of episodes of diarrhoea and of pneumonia in HIV-infected children. The other evaluated the effect of the supplement on the appetite of these children. The supplement contained vitamins A, B complex, C, D, E and folic acid, and the minerals copper, iron, selenium and zinc at levels based on recommended dietary allowances. In the short-term supplementation study HIV-infected children aged 4-24 months who were hospitalized with pneumonia or diarrhoea received the supplement or a placebo until discharge from hospital. The duration of hospitalization was 1.7 days (19%) shorter in the supplement group. Long-term multi-micronutrient supplementation improved the weight-for-age and weight-for-height Z-scores of HIV-infected children aged 4-24 months by 0.4 over the 6-month period. There was no improvement in stunting. Children in the supplement group had substantially fewer episodes of respiratory symptoms per month than the placebo group (0.66 ± 0.51) per month vs (1.01 ± 0.67) (P P = 0.09). There was no effect on CD4 lymphocytes. Long-term supplementation with micronutrients had benefits on the appetite of HIV-infected children aged 6-24 months as well. Improvements in amount of test food eaten over the 6-month period were much higher among children who received the supplement (4.7 ± 14.7 g/kg body weight) than the changes in those who received the placebo (-1.4 ± 11.6 g/kg body weight). Conclusion: Multi-micronutrient supplementation reduces the duration of diarrhoea and of pneumonia and incidence of diarrhoea and of respiratory symptoms in HIV-infected children who are not yet on ART. Multi-micronutrient supplementation also improves appetite and weight in these children but not height. The results of these studies indicate that multi-micronutrient supplementation should be considered in HIV-infected infant and young children who have not commenced ART. </p

The relationship between economic activity and stock market perfomance: evidence from South Africa

A research report submitted to the Faculty of Commerce, Law and Management, University of the Witwatersrand, Johannesburg, In partial fulfilment of the requirements for the degree of Master of Management (Finance and Investment Management), 2016The relationship between real economic activity and stock market performance is one that has been extensively researched throughout many decades, across many economies. Many issues and debates have stemmed involving this relationship, with the major ones including those of the significance of the relationship, nature of the relationship as well as causality and direction of causality within the relationship. This research paper examines this relationship within the South African context, comparing the pre and post 2008 global financial crisis periods. Results both in support of and contrary to theory were found as real economic activity had an immediate postitive response to shocks imposed on the stock index, whilst the stock index had an immediate negative response to shocks imposed on real economic activity. Through the use of granger causality testing, no causality was found in either direction. Furthermore, no major differences were noted between the pre and post crisis periods.GR201

Prevalence and of Paramphistomiasis in Ruminants Slaughtered at Sokoto Central Abattoir, Sokoto

A Survey of prevalence and fluke burden of Paramphistomum sp. was conducted among the major ruminants slaughtered in Sokoto Central Abattoir between May and October, 2007. One hundred (100) of goats, sheep and cattle each were examined for the presence of Paramphistomum species (stomach flukes). Flukes were counted to determine the average fluke burden and prevalence. Out of the 300 animals, a total of 100 (33.3%) were infected with an average fluke burden of 4794. Out of these, 56 (56%) were cattle, with fluke burden of 2517(52.5%}, 32 (32%) were sheep with fluke burden of 1907 (39.8%) and 12 (12%} with fluke burden of 370 (6.7%) were goats. Out of the 100cattle, 20 (20%) males and 36 (36%) females were infected with flukes. Also, out of the 100 sheep, 4 (4%) were males and 28 (28%} were females and in goats, 4 (4%) were males while 8 (8%) were females. On the basis of age the result showed that 4(7.1%) out of the 56 infected animals were those of 1-2 yrs, 40 (71.4%} were 3-4 yrs old and 12 (21.4%) were animals &gt;4 yrs in respect of cattle. Of the 32 infected sheep, 6 (18.7%) were 1-2 yrs, 18(56.2%) were 3-4 yrs and 8(25%) were &gt;4yrs. Similarly, 2(16%) out of the 12 goats infected were 1-2 yrs, 8 (66.6%) were 3-4 yrs old and 2 (16%) were &gt;4 yrs. Paramphistomiasis is prevalent in the area, with female cattle having higher prevalence. Measures forcontrol of these parasites should be prioritized in the study area.Keywords; Prevalence, Paramphistomiasis, Fluke, Burde

Spatio-temporal patterns act as computational mechanisms governing emergent behavior in robotic swarms

Our goal is to control a robotic swarm without removing its swarm-like nature. In other words, we aim to intrinsically control a robotic swarm emergent behavior. Past attempts at governing robotic swarms or their self-coordinating emergent behavior, has proven ineffective, largely due to the swarm's inherent randomness (making it difficult to predict) and utter simplicity (they lack a leader, any kind of centralized control, long-range communication, global knowledge, complex internal models and only operate on a couple of basic, reactive rules). The main problem is that emergent phenomena itself is not fully understood, despite being at the forefront of current research. Research into 1D and 2D Cellular Automata has uncovered a hidden computational layer which bridges the micro-macro gap (i.e., how individual behaviors at the micro-level influence the global behaviors on the macro-level). We hypothesize that there also lie embedded computational mechanisms at the heart of a robotic swarm's emergent behavior. To test this theory, we proceeded to simulate robotic swarms (represented as both particles and dynamic networks) and then designed local rules to induce various types of intelligent, emergent behaviors (as well as designing genetic algorithms to evolve robotic swarms with emergent behaviors). Finally, we analysed these robotic swarms and successfully confirmed our hypothesis; analyzing their developments and interactions over time revealed various forms of embedded spatiotemporal patterns which store, propagate and parallel process information across the swarm according to some internal, collision-based logic (solving the mystery of how simple robots are able to self-coordinate and allow global behaviors to emerge across the swarm)

Gene therapy approaches to disease of the cornea and anterior chamber

The field of ocular gene therapy has become one of the most developed areas within the wider gene therapy field, however most work to date has focused upon the retina with the cornea, by comparison, having seen relatively little application of gene therapy. This thesis describes a program of work to further develop viral gene therapy approaches to the three cellular layers of the cornea, with particular emphasis upon the application of novel vector technologies and overcoming the various challenges presented by each layer. Gene therapy of the corneal endothelium has to date largely aimed to increase or maintain endothelial cell density to improve the quality of donor corneas for engraftment. Such a strategy however carries an inherent risk of oncogenesis and this study has therefore aimed to improve the safety profile of endothelial gene delivery methods. The transduction profile of various AAV serotypes within the corneal stroma was also investigated, and the most promising results applied in an augmentation gene therapy approach to prevent corneal neovascularisation. The selected methodology is shown to mediate high level transgene expression and, when delivering the antiangiogenic factor sFlt1, was highly effective in preventing haem (but not lymph) angiogenesis in a murine model of induced corneal neovascularisation. If long term gene delivery to the corneal epithelium is to be achieved it must be targeted to the limbal epithelial stem cells (LESCs) responsible for the continuous regeneration of the layer. This study has convincingly demonstrated gene delivery to these cells in vivo for the first time, with the methodology developed leading to a lasting transgene expression throughout the LESC daughter cell lineages that comprise the epithelium. In addition to potential application in the treatment of congenital epithelial dystrophies this technique may also provide new insights into LESC biology and the cellular dynamics of epithelial renewal

Epilepsy South Africa, Western Cape, Content Management System: User Manual

Assisting in development of an electronic database to manage client and activity data. The User Manual provides the information necessary for the user to effectively use the automated information system

A Hydrogel-Integrated Culture Device to Interrogate T Cell Activation with Physicochemical Cues

The recent rise of adoptive T cell therapy (ATCT) as a promising cancer immunotherapy has triggered increased interest in therapeutic T cell bioprocessing. T cell activation is a critical processing step and is known to be modulated by physical parameters, such as substrate stiffness. Nevertheless, relatively little is known about how biophysical factors regulate immune cells, such as T cells. Understanding how T cell activation is modulated by physical and biochemical cues may offer novel methods to control cell behavior for therapeutic cell processing. Inspired by T cell mechanosensitivity, we developed a multiwell, reusable, customizable, two-dimensional (2D) polyacrylamide (PA) hydrogel-integrated culture device to study the physicochemical stimulation of Jurkat T cells. Substrate stiffness and ligand density were tuned by concentrations of the hydrogel cross-linker and antibody in the coating solution, respectively. We cultured Jurkat T cells on 2D hydrogels of different stiffnesses that presented surface-immobilized stimulatory antibodies against CD3 and CD28 and demonstrated that Jurkat T cells stimulated by stiff hydrogels (50.6 ± 15.1 kPa) exhibited significantly higher interleukin-2 (IL-2) secretion, but lower proliferation, than those stimulated by softer hydrogels (7.1 ± 0.4 kPa). In addition, we found that increasing anti-CD3 concentration from 10 to 30 μg/mL led to a significant increase in IL-2 secretion from cells stimulated on 7.1 ± 0.4 and 9.3 ± 2.4 kPa gels. Simultaneous tuning of substrate stiffness and stimulatory ligand density showed that the two parameters synergize (two-way ANOVA interaction effect: p < 0.001) to enhance IL-2 secretion. Our results demonstrate the importance of physical parameters in immune cell stimulation and highlight the potential of designing future immunostimulatory biomaterials that are mechanically tailored to balance stimulatory strength and downstream proliferative capacity of therapeutic T cells