PESE: Event Structure Extraction using Pointer Network based Encoder-Decoder Architecture

The task of event extraction (EE) aims to find the events and event-related argument information from the text and represent them in a structured format. Most previous works try to solve the problem by separately identifying multiple substructures and aggregating them to get the complete event structure. The problem with the methods is that it fails to identify all the interdependencies among the event participants (event-triggers, arguments, and roles). In this paper, we represent each event record in a unique tuple format that contains trigger phrase, trigger type, argument phrase, and corresponding role information. Our proposed pointer network-based encoder-decoder model generates an event tuple in each time step by exploiting the interactions among event participants and presenting a truly end-to-end solution to the EE task. We evaluate our model on the ACE2005 dataset, and experimental results demonstrate the effectiveness of our model by achieving competitive performance compared to the state-of-the-art methods

Feature extraction of electrocardiogram signal using machine learning classification

In this article, we'll introduce ways to build virtual worlds through different computer programs. We will show the method of rectangles for analyzing data obtained from the electroencephalogram. We will demonstrate basic mathematical models for movement prediction in a system of virtual reality. Using this data, the main transformations are possible-change of position and rotation (change of orientation)

Production of poly hydroxy butyrate (PHB) from Eichhornia crassipes through microbial fermentation process

Polyhydroxybutyrate (PHB) is one of the highly biodegradable and biologically acceptable thermoplastics synthesized by many microorganisms collectively called polyhydroxyalkanoates (PHAs). All available biopolymers are viewed as perfect answers for the resolution of natural contamination issue by supplanting ordinary plastic business. They are likewise utilized as osteosclerotic stimulants attributable to their piezoelectric properties, in bone plates, during operations as suture material and vein substitutions. Synthesis of PHB is found in a wide range of Gram’s negative and gram’s positive bacteria belonging to distinct genera. Optimum culture condition for the PHB producing microbes are provided, including restricted centralization of nitrogen, sulfur, phosphorus, or the trace elements and maximum convergence of carbon source Indeed, to market PHAs, significant exertion has been dedicated towards a decline in the production cost through the improvement of bacterial strains and enhancing effectiveness of recovery/fermentation procedure. This is being done considering the fact that substrate prices show the greatest impact on PHA's manufacturing cost. The price of the substrate used has the most significant influence on the production cost of PHA. In this research, a potential bacterial strain was isolated from the soil and tested for its PHB producing ability. The use of cheaper substrate for lowering the cost is prerequisite. For PHB production, water hyacinth was used as a carbon source. Bacterial growth was optimized for maximum PHB production. The optimum condition was found to be 30 °C, 8% substrate concentration and 72 h of incubation time

Optimiranje ekstrakcije polifenola iz okare

The objective of the present investigation is to examine okara, a suitable substrate for polyphenol extraction, and to develop a feasible eco-friendly process to maximize the yield of antioxidant phenolics. Box-Behnken design (BBD) based on response surface methodology (RSM) was employed to investigate the effect of temperature (°C), solvent fraction (%) and incubation time (min) on polyphenol extraction by using MINITAB 15 software. Acetone was used as solvent to extract the phenolic compounds possessing the antioxidant properties (DPPH radical scavenging activity, reducing power, and metal chelating activity). Extraction under the optimum conditions yielded total polyphenolic content of 1.16 mg/mL, DPPH radical scavenging activity of 61.07 %, metal chelating activity of 61.20 % and better reducing power. The effective model developed for antioxidant mining from okara under mild operational conditions can be a valuable technique for soybean-based food industry.Svrha je rada bila ispitati svojstva okare, supstrata za dobivanje polifenola, te razviti održivi, ekološki postupak izdvajanja maksimalne količine polifenola. Da bi se ispitao utjecaj temperature, udjela otapala i vremena inkubacije na ekstrakciju polifenola, upotrijebljen je Box-Behnken dizajn metodologije odzivnih površina uz pomoć softvera MINITAB 15. Upotrijebljen je aceton kao otapalo za ekstrakciju fenolnih spojeva, te su ispitana svojstva polifenola, i to: sposobnost uklanjanja DPPH radikala, reducirajuća snaga i aktivnost keliranja metala. Pri optimalnim je uvjetima dobiveno 1,16 mg/mL ukupnih polifenola, s aktivnošću uklanjanja DPPH radikala od 61,07 %; keliranja metala od 61,20 % i dobrom reducirajućom snagom. Razvijen je učinkoviti model izdvajanja antioksidativnih spojeva iz okare u umjerenim uvjetima procesa, što je važno u proizvodnji sojinih proizvoda

CO-INFECTION OF BRUCELLA AND DENGUE VIRUS

Objective: Brucellosis is an important zoonotic infection having a worldwide distribution. In South East Asian developing countries like India, brucellosis is yet well-known but often neglected disease. Although dengue fever has a global distribution, South East Asian region together with the Western Pacific region represents 75% of the current global disease burden. Frequent dengue fever outbreaks have been encountered in different parts of India. Both brucellosis and dengue fever may present with many nonspecific symptoms. This study was designed to identify the trend of their co-infection.Methods: The study was carried out from February 2015 to July 2016 in Eastern India. Blood samples collected from clinically suspected dengue fever cases were confirmed serologically by dengue specific NS1, IgM, and IgG capture ELISA methods. The confirmed dengue samples were further subjected to Brucella specific serological and molecular investigations.Results: Out of 37 serologically confirmed dengue cases, 8 (21.6%) showed co-infection of brucellosis and dengue fever. Female predominance was observed among the co-infection cases. Most of these cases belonged to the age group 21-40 years and resided in and around Kolkata.Conclusion: This study portrays the simultaneous acquisition of these two pathogens in a few patients. Co-infection of these two pathogens, while feasible, has not been accounted previously in this part of India. Thus, this report highlights the significance of studying the possibility of occurrence of dual infection of these two diseases and its outcome in patients with febrile illness thereby providing future scope of preventing case fatalitiesKeywords: Brucellosis, Dengue, Co-infection, ELISA, Polymerase chain reaction

Optimiranje ekstrakcije polifenola iz okare

The objective of the present investigation is to examine okara, a suitable substrate for polyphenol extraction, and to develop a feasible eco-friendly process to maximize the yield of antioxidant phenolics. Box-Behnken design (BBD) based on response surface methodology (RSM) was employed to investigate the effect of temperature (°C), solvent fraction (%) and incubation time (min) on polyphenol extraction by using MINITAB 15 software. Acetone was used as solvent to extract the phenolic compounds possessing the antioxidant properties (DPPH radical scavenging activity, reducing power, and metal chelating activity). Extraction under the optimum conditions yielded total polyphenolic content of 1.16 mg/mL, DPPH radical scavenging activity of 61.07 %, metal chelating activity of 61.20 % and better reducing power. The effective model developed for antioxidant mining from okara under mild operational conditions can be a valuable technique for soybean-based food industry.Svrha je rada bila ispitati svojstva okare, supstrata za dobivanje polifenola, te razviti održivi, ekološki postupak izdvajanja maksimalne količine polifenola. Da bi se ispitao utjecaj temperature, udjela otapala i vremena inkubacije na ekstrakciju polifenola, upotrijebljen je Box-Behnken dizajn metodologije odzivnih površina uz pomoć softvera MINITAB 15. Upotrijebljen je aceton kao otapalo za ekstrakciju fenolnih spojeva, te su ispitana svojstva polifenola, i to: sposobnost uklanjanja DPPH radikala, reducirajuća snaga i aktivnost keliranja metala. Pri optimalnim je uvjetima dobiveno 1,16 mg/mL ukupnih polifenola, s aktivnošću uklanjanja DPPH radikala od 61,07 %; keliranja metala od 61,20 % i dobrom reducirajućom snagom. Razvijen je učinkoviti model izdvajanja antioksidativnih spojeva iz okare u umjerenim uvjetima procesa, što je važno u proizvodnji sojinih proizvoda

Lipase production from a wild (LPF-5) and a mutant (HN1) strain of Aspergillus niger

In this study, a wild (LPF-5) and a mutant (HN1) strain of A. niger were compared for lipase production. Several physical parameters (carbon source, nitrogen source, pH, temperature and incubation period) were optimized for maximization of lipase production. Lipase activity between wild type and mutant strain were compared. Among all carbon sources, mixture of glucose (1%, w/v) and olive oil (1%, v/v) exhibited maximum increase in the production of lipases by both the wild (94.91 ± 0.60 U mL-1 min-1) and mutant (118.23 ± 0.73 U mL-1 min-1) strain. Addition of glucose into the production medium (containing olive oil) increased the production of lipase up to 20% in case of both the strains. The production of lipase by both the strains was higher in the medium of pH 7.0 containing peptone (1%, w/v) as nitrogen source after 3 days of incubation at 28°C. The activity of lipase from HN1 strain in optimized medium was 40% higher (147.65 ± 1.14 U mL-1 min-1) than in un-optimized medium (105.19 ± 0.91 U mL-1 min-1), while it was 38% higher for LPF-5 strain in optimized medium. Therefore the mutant strain (A. niger HN1) is prospective for the development of industrial biotechnology for production of extracellular lipase. Lipase enzyme was partially purified by ammonium sulfate precipitation and 70% precipitate showed highest specific activity of 66.12 U mg-1 for mutant strain as compared to specific activity of 29.88 U mg-1 in crude lysate.Keywords: Wild strain, mutant strain, Aspergillus niger, lipase activity, specific activity, ammonium sulfat

Accessibility of Enzymatically Delignified Bambusa bambos for Efficient Hydrolysis at Minimum Cellulase Loading: An Optimization Study

In the present investigation, Bambusa bambos was used for optimization of enzymatic pretreatment and saccharification. Maximum enzymatic delignification achieved was 84%, after 8 h of incubation time. Highest reducing sugar yield from enzyme-pretreated Bambusa bambos was 818.01 mg/g dry substrate after 8 h of incubation time at a low cellulase loading (endoglucanase, β-glucosidase, exoglucanase, and xylanase were 1.63 IU/mL, 1.28 IU/mL, 0.08 IU/mL, and 47.93 IU/mL, respectively). Enzyme-treated substrate of Bambusa bambos was characterized by analytical techniques such as Fourier transformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The FTIR spectrum showed that the absorption peaks of several functional groups were decreased after enzymatic pretreatment. XRD analysis indicated that cellulose crystallinity of enzyme-treated samples was increased due to the removal of amorphous lignin and hemicelluloses. SEM image showed that surface structure of Bambusa bambos was distorted after enzymatic pretreatment

Osteoblast Behavior on Silicon and Porous-Silicon Substrates

Osteoblast viability, proliferation, protein expression and mineralization were studied on bare, micro- and nanoporous silicon (Si) substrates. Micro- and nano-porous-Si substrates were prepared by anodic etching of silicon in ethanolic hydrofluoric acid and characterized using scanning electron and atomic force microscopies. Mouse osteoblasts were cultured on these substrates and cellular response to these surfaces was assessed using the Live/Dead Cell Viability assay and the MTT assay for cell proliferation. Osteoblast functionality was assessed using immunohistochemistry for bone protein specific markers. Osteoblasts grew well on micro- and nanoporous silicon substrates over the twenty-one day experimental period supporting the assessment that these are suitable cell supportive surfaces. Cell proliferation rates on bare and nanoporous silicon were similar initially, however, nanoporous silicon displayed enhanced cell proliferation, in comparison to bare silicon, after 14 days in culture. Immunocytochemical assays, using bone specific markers, showed positive reactions for osteonectin and osteopontin expression on all substrates with staining intensity increasing over the 21-day experimental period. Calcium mineral deposits were quantified using the Alizarin Red histochemical assay and nanoporous silicon induced the highest level of calcium mineral production in comparison to bare and microporous silicon. The data supports the potential use of nanoporous silicon as a surface implant coating for dental and orthopedic applications. The ability to dope (and then release) drugs or growth factors from the silicon nanopores offers the potential for a multi-functional implant surface