Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment

Dendritic cells play a key role in activation of the immune system as potent antigen-presenting cells. This pivotal position, along with the ability to generate dendritic cells from monocytes and ready uptake of antigen, makes them an intriguing vehicle for immunotherapy for a variety of indications. Since the first reported trial using dendritic cells in 1995, they have been used in trials all over the world for a plethora of indications. Monocyte-derived dendritic cells are generated from whole blood or apheresis products by culturing enriched monocytes in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). A variety of methods can be used for enrichment of monocytes for generation of clinical-grade dendritic cells and are summarized herein

Anomalous dielectric response of very small quantities of virgin, aged and failed silicone oil

A technique is described for making dielectric spectroscopy measurements of very small quantities (<1μl) of oil. The technique utilises surface tension to hold the oil between the plates of a capacitor, the inter-electrode distance being controlled by a micrometer. Breakdown strength can also be estimated using this technique. Three samples of silicone oil, used in cable sealing ends, were tested: virgin, used and failed. A major component in the frequency dependent impedance had the form Z(ω)=B(1-b)(iω)/sup.1-p/). This component was interpreted in terms of a fractal percolation model, and the anomalous thickness dependence predicted by the model verified by varying the inter-electrode distance. The difference observed for the three different samples indicate that conducting contaminants are responsible for the percolation system

On the Complexity of Conceptual Animal Metaphors in Queer Speech

For English speaking members of the queer community in the United States, there is a group of animal terms used to categorize different “tribes” of community members. For example, the term bear generally refers to an individual who is large, hairy, and older. Samardžić (2015) proposes these animal terms as the conceptual metaphor: GAYS ARE ANIMALS. In cognitive linguistics, conceptual metaphor refers to the understanding of an abstract concept in terms of a concrete source. In other words, the different “tribes” of queer community members are conceptually understood in terms of animals. Departing from Samardžić (2015), the present author proposes that the conceptual metaphor, GAYS ARE ANIMALS, is a complex metaphor, meaning it consists of more than one type of metaphor. For example, while terms such as bear and otter primarily refer to one’s physical appearance, other terms such as pig and pup refer to one’s kinks and sexual desires. This would be a simple metaphor if all the animal terms described the same features (e.g. physical appearance). The author analyzes these animal metaphors within the framework of Conceptual Metaphor Theory (Lakoff and Johnson, 1980; Kövecses, 2010) using data collected from online media sources. Very little academic literature exists on the topic of animal metaphors in queer speech, and this project aims to shed light on the complexity of these metaphors. Animal metaphors encode a conceptual understanding of the human experience through the lens of the queer community. By analyzing these metaphors, this project not only contributes to the study of conceptual metaphors, but also contributes to the documentation and study of queer culture and language

An Investigation of Environmental Conditions Experienced During the Life of High Value Wood Components and Products

Australian forest industries have a long history of export trade of a wide range of products from woodchips(for paper manufacturing), sandalwood (essential oils, carving and incense) to high value musical instruments, flooring and outdoor furniture. For the high value group, fluctuating environmental conditions brought on by changes in mperature and relative humidity, can lead to performance problems due to consequential swelling, shrinkage and/or distortion of the wood elements. A survey determined the types of value-added products exported, including species and dimensions packaging used and export markets. Data loggers were installed with shipments to monitor temperature and relative humidity conditions. These data were converted to timber equilibrium moisture content values to provide an indication of the environment that the wood elements would be acclimatising to. The results of the initial survey indicated that primary high value wood export products included guitars, flooring, decking and outdoor furniture. The destination markets were mainly located in the northern hemisphere, particularly the United States of America, China, Hong Kong, Europe including the United Kingdom), Japan, Korea and the Middle East. Other regions importing Australian-made wooden articles were south-east Asia, New Zealand and South Africa. Different timber species have differing rates of swelling and shrinkage, so the types of timber were also recorded during the survey. Results from this work determined that the major species were ash-type eucalypts from south-eastern Australia (commonly referred to in the market as Tasmanian oak), jarrah from Western Australia, spotted gum, hoop pine, white cypress, black butt, brush box and Sydney blue gum from Queensland and New South Wales. The environmental conditions data indicated that microclimates in shipping containers can fluctuate extensively during shipping. Conditions at the time of manufacturing were usually between 10 and 12% equilibrium moisture content, however conditions during shipping could range from 5 (very dry) to 20% (very humid). The packaging systems incorporated were reported to be efficient at protecting the wooden articles from damage during transit. The research highlighted the potential risk for wood components to ‘move’ in response to periods of drier or more humid conditions than those at the time of manufacturing, and the importance of engineering a packaging system that can account for the environmental conditions experienced in shipping containers. Examples of potential dimensional changes in wooden components were calculated based on published unit shrinkage data for key species and the climatic data returned from the logging equipment. The information highlighted the importance of good design to account for possible timber movement during shipping. A timber movement calculator was developed to allow designers to input component species, dimensions, site of manufacture and destination, to see validate their product design. This calculator forms part of the free interactive website www.timbers.com.au

Law and order : Nixon's rhetoric and the Southern strategy

David W. Levy Prize finalist, Spring 2017Today's familiar Democratic and Republican party coalitions have not always existed; rather, they began to emerge in the 1960s as demographic and geographic groups shifted party alliances. This paper focuses on one factor in the party realignment: Richard Nixon's 1968 presidential campaign. Nixon's 1968 campaign was characterized by a balance between appeals to conservative, anti-integration Southern white voters and the risk of alienating Northern liberals. To implement this "Southern strategy," Nixon employed ostensibly race-neutral language that actually had coded racial meaning. This color-blind rhetoric was belied by the actions of the administration and Nixon's rhetorical shift to the right after taking office.undergraduat