Atypical one-carbon metabolism of an acetogenic and hydrogenogenic Moorella thermoacetica strain

A thermophilic spore-forming bacterium (strain AMP) was isolated from a thermophilic methanogenic bioreactor that was fed with cobalt-deprived synthetic medium containing methanol as substrate. 16S rRNA gene analysis revealed that strain AMP was closely related to the acetogenic bacterium Moorella thermoacetica DSM 521T (98.3% sequence similarity). DNA¿DNA hybridization showed 75.2 ± 4.7% similarity to M. thermoacetica DSM 521T, suggesting that strain AMP is a M. thermoacetica strain. Strain AMP has a unique one-carbon metabolism compared to other Moorella species. In media without cobalt growth of strain AMP on methanol was only sustained in coculture with a hydrogen-consuming methanogen, while in media with cobalt it grew acetogenically in the absence of the methanogen. Addition of thiosulfate led to sulfide formation and less acetate formation. Growth of strain AMP with CO resulted in the formation of hydrogen as the main product, while other CO-utilizing Moorella strains produce acetate as product. Formate supported growth only in the presence of thiosulfate or in coculture with the methanogen. Strain AMP did not grow with H2/CO2, unlike M. thermoacetica (DSM 521T). The lack of growth with H2/CO2 likely is due to the absence of cytochrome b in strain AM

Carboxydotrophic growth of <i>Geobacter sulfurreducens</i>

This study shows that Geobacter sulfurreducensgrows on carbon monoxide (CO) as electron donor with fumarateas electron acceptor. Geobacter sulfurreducens wastolerant to high CO levels, with up to 150 kPa in the headspacetested. During growth, hydrogen was detected in very slightamounts (~5 Pa). In assays with cell-free extract of cellsgrown with CO and fumarate, production of hydrogen fromCO was not observed, and hydrogenase activity with benzylviologen as electron acceptor was very low. Taken together,this suggested that CO is not utilized via hydrogen as intermediate.In the presence of CO, reduction of NADP+ wasobserved at a rate comparable to CO oxidation coupled tofumarate reduction in vivo. The G. sulfurreducens genomecontains a single putative carbon monoxide dehydrogenaseencodinggene. The gene is part of a predicted operon alsocomprising a putative Fe–S cluster-binding subunit (CooF)and a FAD–NAD(P) oxidoreductase and is preceded by aputative CO-sensing transcription factor. This cluster may beinvolved in a novel pathway for CO oxidation, but furtherstudies are necessary to ascertain this. Similar gene clustersare present in several other species belonging to theDeltaproteobacteria and Firmicutes, for which CO utilizationis currently not known

Deep Conversion of Carbon Monoxide to Hydrogen and Formation of Acetate by the Anaerobic Thermophile Carboxydothermus hydrogenoformans

Carboxydothermus hydrogenoformans is a thermophilic strictly anaerobic bacterium that catalyses the water gas shift reaction, the conversion of carbon monoxide with water to molecular hydrogen and carbon dioxide. The thermodynamically favorable growth temperature, compared to existing industrial catalytic processes, makes this organism an interesting alternative for production of cheap hydrogen gas suitable to fuel CO-sensitive fuel cells in a future hydrogen economy, provided sufficiently low levels of CO are reached. Here we study CO conversion and final CO levels in cultures of C. hydrogenoformans grown in batch cultures that were started with a 100% CO gas phase with and without removal of formed CO2. Final CO levels were 117 ppm without CO2 removal and below 2 ppm with CO2 removal. The Gibbs free energy change calculated with measured end concentrations and the detection of acetate suggest that C. hydrogenoformans shifted from a hydrogenogenic to an acetogenic metabolism

Energy-dispersive X-ray analysis on thin sections and unimpregnated soil material.

A combination of scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDXRA) was used in the study of soil materials. The investigation in situ of components in thin sections was used to estimate chemical elements with atomic numbers 11 upwards, from sodium on. EDXRA could detect chemical elements up to magnifications of X 10 000. The composition of amorphous and micro-crystalline materials cannot be estimated in thin sections by light microscopy but by this technique was clearly displayed. Composition of loose soil material can also be investigated. The material that could be studied by SEM-EDXRA did not need high polishing of the thin section, and the plastic used for impregnation of the soil material was not affected by the investigation.Identification of chemical elements in situ, high resolution of the topographic image and relatively short testing times for the elements make this combination of techniques useful for soil research. (Abstract retrieved from CAB Abstracts by CABI’s permission

Horace Walpole and his correspondents : social network analysis in a historical context

Horace Walpole (1717-1797) was not only a politician, author and patron of the arts, he was also one of the most prolific letter writers in all of English history. Many of the letters which he sent and received have been collected, edited and published. They are a treasure trove of information on eighteenth-century politics, arts and society. However, the current study rather focuses on Walpole__s social network and the language as contained in the letters of the network members. Although Walpole and his correspondents wrote their letters during a period in which the standard language was being established and written down in grammars, this book challenges the view that the language use of these members of the upper class was uniformly standard. Several case studies are used to map linguistic variety in the network and demonstrate the functionality of social network analysis in a historical context. The aim is to establish network structures in order to explain usage variation within the network. The author provides an overview of earlier and more recent work on the historical application of social network analysis, and furthermore suggests an adaptation of the model for improved functionality when used with historical dataDescriptive and Comparative Linguistic

Het teken van het beest : IJje Wijkstra en de geschiedenis van de viervoudige politiemoord, 18 januari 1929

On January 18th 1929 four policemen went to meet Aaltje Wobbes-van der Tuin who had recently moved in with IJje Wijkstra, who had lived by himself in the small village named Doezum in the south-western part of the province Groningen. Aaltje’s husband was imprisoned for theft. She took all her assets with her except her six under aged children which was a criminal offence. Within the hour of their arrival the policemen lay dead in the snow with their throats slit. Wijkstra had set his house on fire and fled the scene but was arrested later on that day. Upon being asked why he did it, he replied ‘they provoked me.’ This murder caused a lot of commotion. This study is about the life and times of the convicted murderer Wijkstra. Specifically his community is being studied in order to answer the question how and under what circumstances the principal players got caught in this fatal conflict and what role his community played as a third party therein. The unprecedented nature and size of this tragedy demanded an explanation. A further question in this study is which explanations were offered by the different parties involved and what conclusions that warrants.LEI Universiteit LeidenPolitical Culture and National Identit

The gastrointestinal microbiota in colorectal cancer cell migration and invasion

Colorectal carcinoma is the third most common cancer in developed countries and the second leading cause of cancer-related mortality. Interest in the influence of the intestinal microbiota on CRC emerged rapidly in the past few years, and the close presence of microbiota to the tumour mass creates a unique microenvironment in CRC. The gastrointestinal microbiota secrete factors that can contribute to CRC metastasis by influencing, for example, epithelial-to-mesenchymal transition. Although the role of EMT in metastasis is well-studied, mechanisms by which gastrointestinal microbiota contribute to the progression of CRC remain poorly understood. In this review, we will explore bacterial factors that contribute to the migration and invasion of colorectal carcinoma and the mechanisms involved. Bacteria involved in the induction of metastasis in primary CRC include Fusobacterium nucleatum, Enterococcus faecalis, enterotoxigenic Bacteroides fragilis, Escherichia coli and Salmonella enterica. Examples of prominent bacterial factors secreted by these bacteria include Fusobacterium adhesin A and Bacteroides fragilis Toxin. Most of these factors induce EMT-like properties in carcinoma cells and, as such, contribute to disease progression by affecting cell-cell adhesion, breakdown of the extracellular matrix and reorganisation of the cytoskeleton. It is of utmost importance to elucidate how bacterial factors promote CRC recurrence and metastasis to increase patient survival. So far, mainly animal models have been used to demonstrate this interplay between the host and microbiota. More human-based models are needed to study the mechanisms that promote migration and invasion and mimic the progression and recurrence of CRC