Using triggers in a practice-led research methodology to challenge the conventions of communication design.

In this research, I am using an understanding of visual communication that holds that design is more than a spontaneous creative act – it can be studied and used as part of a research project that examines the design process itself . This project seeks to re-position design as a mode of questioning, with an emphasis on exploring the nature of communication design practice and how to extend it, rather than creating and reflecting on new design activities. The works explore assumptions about design and provokes a fresh consideration of communication design practice that will have operational significance. To appropriate words used to describe the work of the 2011 Nobel Prize winner for Literature, Tomas Tranströmer, I was looking for a design approach that would ‘find a fresh way into reality’. As Faust states, ‘When we reframe design through a discourse, designing on a meta level, we are actually designing design, as we are giving design a different meaning, changing frame to include or exclude what we do or don’t consider as a part of the field’ (Faust 2010, p. 109). This highlights the potential of a design to reframe communication design and to broaden traditional boundaries to trigger a challenge to the conventions of communication design. This moves the conversation about sustainable design away from discussions of materials and message, and onto the underlying ideas of design culture and the frameworks that create received ideas about design norms. The research process helped me reimagine the role of both the designer and design audience. This kind of exploration is an underdeveloped area in sustainable design research. Sustainable design often fails to reference a wider consideration of the conceptual framework of communication design practice. Sustainable design needs to engage with the culture of consumption and its unsustainable demand for resources (Jackson 2009) and the social effects of communication design. The concepts of sustainable communication design practice are largely undeveloped, beyond minimising the environmental impact of communication design within a commercial context – for example, by using paper from a sustainable source, using soya-based inks, minimising the practice’s energy use and avoiding working for clients with environmentally unsound practices. Although these considerations are important, their scope is not broad enough to address or halt the damage being done to the world’s ecosystems (United Nations 2011, Jackson 2009). This research attempts to harness the full potential of communication designers to create social change through critical engagement with design practice

Dlx2 over-expression regulates cell adhesion and mesenchymal condensation in ectomesenchyme

AbstractThe Dlx family of homeodomain transcription factors have diverse roles in development including craniofacial morphogenesis and consists of 6 members with overlapping expression patterns. Dlx2 is expressed within the developing branchial arches in both the epithelium and mesenchyme and targeted deletion in mice has revealed roles in patterning and development of the craniofacial skeleton. Defects in Dlx2 null mice include skeletal anomalies of proximal branchial arch 1 derivatives while distal elements are largely spared indicating redundancy within the Dlx family. We have investigated the function of Dlx2 using in ovo electroporation and cell culture. Ectopic expression of Dlx2 within the neural tube beginning prior to emigration of neural crest cells at E1.25 drastically inhibits the migration of transfected cells and induces aggregation of transfected neuroepithelial cells within the neural tube at 24 h post-electroporation. By 48 h post-electroporation, the majority of transfected cells formed multicellular aggregates that were found adjacent to the basal side of the neural tube and very few Dlx2 expressing cells migrated to the level of the branchial arches. Similar results were obtained for Dlx5, suggesting these effects may be common to Dlx genes. Electroporation of the Dlx2 expression construct into branchial arch mesenchyme induced N-cadherin and NCAM, a dramatic increase in cell–cell adhesion relative to controls, and resulted in an increase in mesenchymal condensation. These results suggest a role for Dlx genes in regulating ectomesenchymal cell adhesion and supports the possibility that the skeletal dysmorphology seen in Dlx null mice may derive from abnormalities at the condensation stage

Physiotherapy students and clinical educators perceive several ways in which incorporating peer-assisted learning could improve clinical placements: a qualitative study

AbstractQuestion: What are the experiences of students and clinical educators in a paired student placement model incorporating facilitated peer-assisted learning (PAL) activities, compared to a traditional paired teaching approach? Design: Qualitative study utilising focus groups. Participants: Twenty-four physiotherapy students and 12 clinical educators. Intervention: Participants in this study had experienced two models of physiotherapy clinical undergraduate education: a traditional paired model (usual clinical supervision and learning activities led by clinical educators supervising pairs of students) and a PAL model (a standardised series of learning activities undertaken by student pairs and clinical educators to facilitate peer interaction using guided strategies). Results: Peer-assisted learning appears to reduce the students’ anxiety, enhance their sense of safety in the learning environment, reduce educator burden, maximise the use of downtime, and build professional skills including collaboration and feedback. While PAL adds to the clinical learning experience, it is not considered to be a substitute for observation of the clinical educator, expert feedback and guidance, or hands-on immersive learning activities. Cohesion of the student-student relationship was seen as an enabler of successful PAL. Conclusion: Students and educators perceive that PAL can help to position students as active learners through reduced dependence on the clinical educator, heightened roles in observing practice, and making and communicating evaluative judgments about quality of practice. The role of the clinical educator is not diminished with PAL, but rather is central in designing flexible and meaningful peer-based experiences and in balancing PAL with independent learning opportunities. Registration: ACTRN12610000859088. [Sevenhuysen S, Farlie MK, Keating JL, Haines TP, Molloy E (2015) Physiotherapy students and clinical educators perceive several ways in which incorporating peer-assisted learning could improve clinical placements: a qualitative study. Journal of Physiotherapy 61: 87–92

Global comparative transcriptome analysis of cartilage formation in vivo

<p>Abstract</p> <p>Background</p> <p>During vertebrate embryogenesis the initial stages of bone formation by endochondral ossification involve the aggregation and proliferation of mesenchymal cells into condensations. Continued growth of the condensations and differentiation of the mesenchymal cells into chondrocytes results in the formation of cartilage templates, or anlagen, which prefigure the shape of the future bones. The chondrocytes in the anlagen further differentiate by undergoing a complex sequence of maturation and hypertrophy, and are eventually replaced by mineralized bone. Regulation of the onset of chondrogenesis is incompletely understood, and would be informed by comprehensive analyses of <it>in vivo </it>gene expression.</p> <p>Results</p> <p>Tibial and fibular pre-condensed mesenchyme was microdissected from mouse hind limbs at 11.5 dpc, and the corresponding condensations at 12.5 dpc and cartilage anlagen at 13.5 dpc. Total RNA was isolated, and cRNA generated by linear amplification was interrogated using mouse whole genome microarrays. Differential expression was validated by quantitative PCR for <it>Agc1</it>, <it>Bmp8a</it>, <it>Col2a1</it>, <it>Fgfr4</it>, <it>Foxa3</it>, <it>Gdf5</it>, <it>Klf2</it>, <it>Klf4</it>, <it>Lepre1</it>, <it>Ncad</it>, <it>Sox11</it>, and <it>Trpv4</it>. Further, independent validation of the microarray data was achieved by <it>in situ </it>hybridization to analyse the expression of <it>Lepre1</it>, <it>Pcdh8</it>, <it>Sox11</it>, and <it>Trpv4 </it>from 11.5 dpc to 13.5 dpc during mouse hind limb development. We found significant differential expression of 931 genes during these early stages of chondrogenesis. Of these, 380 genes were down-regulated and 551 up-regulated. Our studies characterized the expression pattern of gene families previously associated with chondrogenesis, such as adhesion molecules, secreted signalling molecules, transcription factors, and extracellular matrix components. Gene ontology approaches identified 892 differentially expressed genes not previously identified during the initiation of chondrogenesis. These included several <it>Bmp, Gdf, Wnt, Sox and Fox </it>family members.</p> <p>Conclusion</p> <p>These data represent the first global gene expression profiling analysis of chondrogenic tissues during <it>in vivo </it>development. They identify genes for further study on their functional roles in chondrogenesis, and provide a comprehensive and important resource for future studies on cartilage development and disease.</p

The Use of Official Statistics in Self-Selection Bias Modeling

Official statistics are a fundamental source of publicly available information that periodically provides a great amount of data on all major areas of citizens’ lives, such as economics, social development, education, and the environment. However, these extraordinary sources of information are often neglected, especially by business and industrial statisticians. In particular, data collected from small businesses, like small and medium-sized enterprizes (SMEs), are rarely integrated with official statistics data

Generalized Logistic Models and its orthant tail dependence

The Multivariate Extreme Value distributions have shown their usefulness in environmental studies, financial and insurance mathematics. The Logistic or Gumbel-Hougaard distribution is one of the oldest multivariate extreme value models and it has been extended to asymmetric models. In this paper we introduce generalized logistic multivariate distributions. Our tools are mixtures of copulas and stable mixing variables, extending approaches in Tawn (1990), Joe and Hu (1996) and Foug\`eres et al. (2009). The parametric family of multivariate extreme value distributions considered presents a flexible dependence structure and we compute for it the multivariate tail dependence coefficients considered in Li (2009)

On the deformability of Heisenberg algebras

Based on the vanishing of the second Hochschild cohomology group of the enveloping algebra of the Heisenberg algebra it is shown that differential algebras coming from quantum groups do not provide a non-trivial deformation of quantum mechanics. For the case of a q-oscillator there exists a deforming map to the classical algebra. It is shown that the differential calculus on quantum planes with involution, i.e. if one works in position-momentum realization, can be mapped on a q-difference calculus on a commutative real space. Although this calculus leads to an interesting discretization it is proved that it can be realized by generators of the undeformed algebra and does not posess a proper group of global transformations.Comment: 16 pages, latex, no figure