Bunker silo safety

Bunker silo safety measures must be in place to be safety compliant

The German Vision of Industry 4.0 Applied in Organic Farming

The first industrial revolution was the invention of the steam engine. With the advent of conveyor belts and electricity, the second industrial revolution arose. After the third revolution, the automation, the fourth industrial revolution takes place with the complete networking of all machines, workers, consumers, and products. In Germany, this is called Industry 4.0. Increasing digitization makes it possible to collect, store, analyze, and communicate large amounts of data. By digitizing farms, a network of different sensors can analyze the nutrient content and the soil texture in real time. This information can be evaluated and the plant distribution can be managed across all networked farms. This leads to the right field being used for the right plant at the right time. Real-time data processing makes it possible to monitor and control the nutrient intake over the entire growth period. This allows the field to specifically ask for water or the right fertilizer for its plants. This saves resources and protects the environment. All the prepared information can give the farmer an exact status about his products and fields via an interface. This horizontal networking within the farm and the vertical networking across different farms can lead to increased efficiency and cheaper products. The use of robots can create a fully automatic farm. For this undertaking, it is necessary to process the complex information of a farm with a self-learning system. At the Westcoast University of Applied Science, for example, a robot is being researched to automatically remove the weeds. The prototype of the robot that moves fully autonomously across the field classifies the plants and destroys the weeds

Manure injection grows corn silage yields

This information was part of the February 2011 issue of Eastern DairyBusiness Magazine. The Manager, a section within the Eastern DairyBusiness Magazine, is authored and organized by the PRO-DAIRY program in the College of Agriculture and Life Sciences at Cornell University

A novel role for the TIR domain in association with pathogen-derived elicitors

Plant innate immunity is mediated by Resistance (R) proteins, which bear a striking resemblance to animal molecules of similar function. Tobacco N is a TIR-NB-LRR R gene that confers resistance to Tobacco mosaic virus, specifically the p50 helicase domain. An intriguing question is how plant R proteins recognize the presence of pathogen-derived Avirulence (Avr) elicitor proteins. We have used biochemical cell fraction and immunoprecipitation in addition to confocal fluorescence microscopy of living tissue to examine the association between N and p50. Surprisingly, both N and p50 are cytoplasmic and nuclear proteins, and N's nuclear localization is required for its function. We also demonstrate an in planta association between N and p50. Further, we show that N's TIR domain is critical for this association, and indeed, it alone can associate with p50. Our results differ from current models for plant innate immunity that propose detection is mediated solely through the LRR domains of these molecules. The data we present support an intricate process of pathogen elicitor recognition by R proteins involving multiple subcellular compartments and the formation of multiple protein complexes. © 2007 Burch-Smith et al

High-resolution imaging of kidney vascular corrosion casts with nano-CT

A vascular corrosion cast of an entire mouse kidney was scanned with a modular multiresolution X-ray nanotomography system. Using an isotropic voxel pitch of 0.5 mu m, capillary systems such as the vasa recta, peritubular capillaries and glomeruli were clearly resolved. This represents a considerable improvement over corrosion casts scanned with microcomputed tomography systems. The resolving power of this system was clearly demonstrated by the unique observation of a dense, subcapsular mat of capillaries enveloping the entire outer surface of the cortical region. Resolution of glomerular capillaries was comparable to similar models derived from laser scanning confocal microscopy. The high-resolution, large field of view and the three-dimensional nature of the resulting data opens new possibilities for the use of corrosion casting in research

Marek's Disease Virus VP22: Subcellular Localization and Characterization of Carboxyl Terminal Deletion Mutations

AbstractMarek's disease virus (MDV) is an alphaherpesvirus that causes T cell lymphoma and severe immunosuppression in chickens. The MDV UL49 gene, which encodes the tegument viral protein 22 (VP22), has been expressed as a green fluorescent protein (GFP) fusion protein in chicken embryonic fibroblasts to examine its subcellular localization. As with both human herpesvirus 1 and bovine herpesvirus 1VP22-GFP fusion proteins, the MDV VP22-GFP product binds to microtubules and heterochromatin. In addition, the MDV protein also binds to the centrosomes. During mitosis, VP22-GFP binds to sister chromatids, but dissociates from the centrosomes and the microtubules of the mitotic spindle. A series of VP22 carboxy terminal truncation mutants were constructed to define regions responsible for these binding properties. These mutants identified separable domains or motifs responsible for binding microtubules and heterochromatin

Considerations for Dairy Farms Regarding Use of Sewage Sludges, Sludge Products and Septage

Sewage sludges and sludge-based products can be used as agricultural soil amendments. They can provide a free or low-cost source of organic matter, nutrients and sometimes lime. Those entities responsible for sludge management provide farmers with their assessment of the benefits of land application. There are specific considerations regarding sludge use on dairy farms for animal, human, soil and plant health as well as for relationships with neighbors. This document is intended to help dairy farmers and their advisors make informed decisions regarding the use of sewage sludges, sludge-based products and septage and to outline measures that can reduce the risks that may be associated with application on dairy farms