19 research outputs found
Quantitative Registration and Distribution Analysis of Multicolor 3DÂ Super-Resolution Images of Proteins Reveals Nanoscale Spatial Organization
Quantitative Multicolor Subdiffraction Imaging of Bacterial Protein Ultrastructures in Three Dimensions
We demonstrate quantitative multicolor
three-dimensional (3D) subdiffraction
imaging of the structural arrangement of fluorescent protein fusions
in living <i>Caulobacter crescentus</i> bacteria. Given
single-molecule localization precisions of 20–40 nm, a flexible
locally weighted image registration algorithm is critical to accurately
combine the super-resolution data with <10 nm error. Surface-relief
dielectric phase masks implement a double-helix response at two wavelengths
to distinguish two different fluorescent labels and to quantitatively
and precisely localize them relative to each other in 3D
Quantitative Multicolor Subdiffraction Imaging of Bacterial Protein Ultrastructures in Three Dimensions
We demonstrate quantitative multicolor
three-dimensional (3D) subdiffraction
imaging of the structural arrangement of fluorescent protein fusions
in living <i>Caulobacter crescentus</i> bacteria. Given
single-molecule localization precisions of 20–40 nm, a flexible
locally weighted image registration algorithm is critical to accurately
combine the super-resolution data with <10 nm error. Surface-relief
dielectric phase masks implement a double-helix response at two wavelengths
to distinguish two different fluorescent labels and to quantitatively
and precisely localize them relative to each other in 3D
Quantitative Multicolor Subdiffraction Imaging of Bacterial Protein Ultrastructures in Three Dimensions
We demonstrate quantitative multicolor
three-dimensional (3D) subdiffraction
imaging of the structural arrangement of fluorescent protein fusions
in living <i>Caulobacter crescentus</i> bacteria. Given
single-molecule localization precisions of 20–40 nm, a flexible
locally weighted image registration algorithm is critical to accurately
combine the super-resolution data with <10 nm error. Surface-relief
dielectric phase masks implement a double-helix response at two wavelengths
to distinguish two different fluorescent labels and to quantitatively
and precisely localize them relative to each other in 3D
Rigorous science demands support of transgender scientists
To build a just, equitable, and diverse academy, scientists and institutions must address systemic barriers that sex and gender minorities face. This Commentary summarizes (1) critical context informing the contemporary oppression of transgender people, (2) how this shapes extant research on sex and gender, and (3) actions to build an inclusive and rigorous academy for all