289 research outputs found
Spherically Symmetric Solutions in Macroscopic Gravity
Schwarzschild's solution to the Einstein Field Equations was one of the first
and most important solutions that lead to the understanding and important
experimental tests of Einstein's theory of General Relativity. However,
Schwarzschild's solution is essentially based on an ideal theory of
gravitation, where all inhomogeneities are ignored. Therefore, any
generalization of the Schwarzschild solution should take into account the
effects of small perturbations that may be present in the gravitational field.
The theory of Macroscopic Gravity characterizes the effects of the
inhomogeneities through a non-perturbative and covariant averaging procedure.
With similar assumptions on the geometry and matter content, a solution to the
averaged field equations as dictated by Macroscopic Gravity are derived. The
resulting solution provides a possible explanation for the flattening of
galactic rotation curves, illustrating that Dark Matter is not real but may
only be the result of averaging inhomogeneities in a spherically symmetric
background.Comment: 14 pages, added and updated references, some paragraphs rewritten for
clarity, typographical errors fixed, results have not change
Isotropic singularity in inhomogeneous brane cosmological models
We discuss the asymptotic dynamical evolution of spatially inhomogeneous
brane-world cosmological models close to the initial singularity. By
introducing suitable scale-invariant dependent variables and a suitable gauge,
we write the evolution equations of the spatially inhomogeneous brane
cosmological models with one spatial degree of freedom as a system of
autonomous first-order partial differential equations. We study the system
numerically, and we find that there always exists an initial singularity, which
is characterized by the fact that spatial derivatives are dynamically
negligible. More importantly, from the numerical analysis we conclude that
there is an initial isotropic singularity in all of these spatially
inhomogeneous brane cosmologies for a range of parameter values which include
the physically important cases of radiation and a scalar field source. The
numerical results are supported by a qualitative dynamical analysis and a
calculation of the past asymptotic decay rates. Although the analysis is local
in nature, the numerics indicates that the singularity is isotropic for all
relevant initial conditions. Therefore this analysis, and a preliminary
investigation of general inhomogeneous () models, indicates that it is
plausible that the initial singularity is isotropic in spatially inhomogeneous
brane-world cosmological models and consequently that brane cosmology naturally
gives rise to a set of initial data that provide the conditions for inflation
to subsequently take place.Comment: 32 pages with 8 pictures. submitted to Class. Quant. Gra
Galiellalactone Inhibits Stem Cell-Like ALDH-Positive Prostate Cancer Cells
Galiellalactone is a potent and specific inhibitor of STAT3 signaling which has been shown to possess growth inhibitory effects on prostate cancer cells expressing active STAT3. In this study we aimed to investigate the effect of galiellalactone on prostate cancer stem cell-like cells. We explored the expression of aldehyde dehydrogenase (ALDH) as a marker for cancer stem cell-like cells in different human prostate cancer cell lines and the effects of galiellalactone on ALDH expressing (ALDH+) prostate cancer cells. ALDH+ subpopulations were detected and isolated from the human prostate cancer cell lines DU145 and long-term IL-6 stimulated LNCaP cells using ALDEFLUOR® assay and flow cytometry. In contrast to ALDH− cells, ALDH+ prostate cancer cells showed cancer stem cell-like characteristics such as increased self-renewing and colony forming capacity and tumorigenicity. In addition, ALDH+ cells showed an increased expression of putative prostate cancer stem cell markers (CD44 and integrin α2β1). Furthermore, ALDH+ cells expressed phosphorylated STAT3. Galiellalactone treatment decreased the proportion of ALDH+ prostate cancer cells and induced apoptosis of ALDH+ cells. The gene expression of ALDH1A1 was downregulated in vivo in galiellalactone treated DU145 xenografts. These findings emphasize that targeting the STAT3 pathway in prostate cancer cells, including prostate cancer stem cell-like cells, is a promising therapeutic approach and that galiellalactone is an interesting compound for the development of future prostate cancer drugs
The aldehyde dehydrogenase enzyme 7A1 is functionally involved in prostate cancer bone metastasis
High aldehyde dehydrogenase (ALDH) activity can be used to identify tumor-initiating and metastasis-initiating cells in various human carcinomas, including prostate cancer. To date, the functional importance of ALDH enzymes in prostate carcinogenesis, progression and metastasis has remained elusive. Previously we identified strong expression of ALDH7A1 in human prostate cancer cell lines, primary tumors and matched bone metastases. In this study, we evaluated whether ALDH7A1 is required for the acquisition of a metastatic stem/progenitor cell phenotype in human prostate cancer. Knockdown of ALDH7A1 expression resulted in a decrease of the α2hi/αvhi/CD44+ stem/progenitor cell subpopulation in the human prostate cancer cell line PC-3M-Pro4. In addition, ALDH7A1 knockdown significantly inhibited the clonogenic and migratory ability of human prostate cancer cells in vitro. Furthermore, a number of genes/factors involved in migration, invasion and metastasis were affected including transcription factors (snail, snail2, and twist) and osteopontin, an ECM molecule involved in metastasis. Knockdown of ALDH7A1 resulted in decreased intra-bone growth and inhibited experimentally induced (bone) metastasis, while intra-prostatic growth was not affected. In line with these observations, evidence is presented that TGF-β, a key player in cancer invasiveness and bone metastasis, strongly induced ALDH activity while BMP7 (an antagonist of TGF-β signaling) down-regulated ALDH activity. Our findings show, for the first time, that the ALDH7A1 enzyme is functionally involved in the formation of bone metastases and that the effect appeared dependent on the microenvironment, i.e., bone versus prostate
On the Asymptotic Behaviour of Cosmological Models in Scalar-Tensor Theories of Gravity
We study the qualitative properties of cosmological models in scalar-tensor
theories of gravity by exploiting the formal equivalence of these theories with
general relativity minimally coupled to a scalar field under a conformal
transformation and field redefinition. In particular, we investigate the
asymptotic behaviour of spatially homogeneous cosmological models in a class of
scalar-tensor theories which are conformally equivalent to general relativistic
Bianchi cosmologies with a scalar field and an exponential potential whose
qualitative features have been studied previously. Particular attention is
focussed on those scalar-tensor theory cosmological models, which are shown to
be self-similar, that correspond to general relativistic models that play an
important r\^{o}le in describing the asymptotic behaviour of more general
models (e.g., those cosmological models that act as early-time and late-time
attractors).Comment: 22 pages, submitted to Phys Rev
The Dynamics of Brane-World Cosmological Models
Brane-world cosmology is motivated by recent developments in string/M-theory
and offers a new perspective on the hierarchy problem. In the brane-world
scenario, our Universe is a four-dimensional subspace or {\em brane} embedded
in a higher-dimensional {\em bulk} spacetime. Ordinary matter fields are
confined to the brane while the gravitational field can also propagate in the
bulk, leading to modifications of Einstein's theory of general relativity at
high energies. In particular, the Randall-Sundrum-type models are
self-consistent and simple and allow for an investigation of the essential
non-linear gravitational dynamics. The governing field equations induced on the
brane differ from the general relativistic equations in that there are nonlocal
effects from the free gravitational field in the bulk, transmitted via the
projection of the bulk Weyl tensor, and the local quadratic energy-momentum
corrections, which are significant in the high-energy regime close to the
initial singularity. In this review we discuss the asymptotic dynamical
evolution of spatially homogeneous brane-world cosmological models containing
both a perfect fluid and a scalar field close to the initial singularity. Using
dynamical systems techniques it is found that, for models with a physically
relevant equation of state, an isotropic singularity is a past-attractor in all
orthogonal spatially homogeneous models (including Bianchi type IX models). In
addition, we describe the dynamics in a class of inhomogeneous brane-world
models, and show that these models also have an isotropic initial singularity.
These results provide support for the conjecture that typically the initial
cosmological singularity is isotropic in brane-world cosmology.Comment: Einstein Centennial Review Article: to appear in CJ
CRIPTO and its signaling partner GRP78 drive the metastatic phenotype in human osteotropic prostate cancer
CRIPTO (CR-1, TDGF1) is a cell surface/secreted oncoprotein actively involved in development and cancer. Here, we report that high expression of CRIPTO correlates with poor survival in stratified risk groups of prostate cancer (PCa) patients. CRIPTO and its signaling partner glucose-regulated protein 78 (GRP78) are highly expressed in PCa metastases and display higher levels in the metastatic ALDHhigh sub-population of PC-3M-Pro4Luc2 PCa cells compared with non-metastatic ALDHlow. Coculture of the osteotropic PC-3M-Pro4Luc2 PCa cells with differentiated primary human osteoblasts induced CRIPTO and GRP78 expression in cancer cells and increases the size of the ALDHhigh sub-population. Additionally, CRIPTO or GRP78 knockdown decreases proliferation, migration, clonogenicity and the size of the metastasis-initiating ALDHhigh sub-population. CRIPTO knockdown reduces the invasion of PC-3M-Pro4Luc2 cells in zebrafish and inhibits bone metastasis in a preclinical mouse model. These results highlight a functional role for CRIPTO and GRP78 in PCa metastasis and suggest that targeting CRIPTO/GRP78 signaling may have significant therapeutic potential.Oncogene advance online publication, 10 April 2017; doi:10.1038/onc.2017.87
Equation of state for Universe from similarity symmetries
In this paper we proposed to use the group of analysis of symmetries of the
dynamical system to describe the evolution of the Universe. This methods is
used in searching for the unknown equation of state. It is shown that group of
symmetries enforce the form of the equation of state for noninteracting scaling
multifluids. We showed that symmetries give rise the equation of state in the
form and energy density
, which
is commonly used in cosmology. The FRW model filled with scaling fluid (called
homological) is confronted with the observations of distant type Ia supernovae.
We found the class of model parameters admissible by the statistical analysis
of SNIa data. We showed that the model with scaling fluid fits well to
supernovae data. We found that and (), which can correspond to (hyper) phantom fluid, and to a
high density universe. However if we assume prior that
then the favoured model is close to concordance
CDM model. Our results predict that in the considered model with
scaling fluids distant type Ia supernovae should be brighter than in
CDM model, while intermediate distant SNIa should be fainter than in
CDM model. We also investigate whether the model with scaling fluid is
actually preferred by data over CDM model. As a result we find from
the Akaike model selection criterion prefers the model with noninteracting
scaling fluid.Comment: accepted for publication versio
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