Cosmic-ray energy spectrum and composition up to the ankle - the case for a second Galactic component

We have carried out a detailed study to understand the observed energy spectrum and composition of cosmic rays with energies up to ~10^18 eV. Our study shows that a single Galactic component with subsequent energy cut-offs in the individual spectra of different elements, optimised to explain the observed spectra below ~10^14 eV and the knee in the all-particle spectrum, cannot explain the observed all-particle spectrum above ~2x10^16 eV. We discuss two approaches for a second component of Galactic cosmic rays -- re-acceleration at a Galactic wind termination shock, and supernova explosions of Wolf-Rayet stars, and show that the latter scenario can explain almost all observed features in the all-particle spectrum and the composition up to ~10^18 eV, when combined with a canonical extra-galactic spectrum expected from strong radio galaxies or a source population with similar cosmological evolution. In this two-component Galactic model, the knee at ~ 3x10^15 eV and the second knee at ~10^17 eV in the all-particle spectrum are due to the cut-offs in the first and second components, respectively. We also discuss several variations of the extra-galactic component, from a minimal contribution to scenarios with a significant component below the ankle (at ~4x10^18 eV), and find that extra-galactic contributions in excess of regular source evolution are neither indicated nor in conflict with the existing data. Our main result is that the second Galactic component predicts a composition of Galactic cosmic rays at and above the second knee that largely consists of helium or a mixture of helium and CNO nuclei, with a weak or essentially vanishing iron fraction, in contrast to most common assumptions. This prediction is in agreement with new measurements from LOFAR and the Pierre Auger Observatory which indicate a strong light component and a rather low iron fraction between ~10^17 and 10^18 eV.Comment: Added Table 4; Published in A&A, 595 (2016) A33 (Highlight paper

Enhancement of quasiparticle recombination in Ta and Al superconductors by implantation of magnetic and nonmagnetic atoms

The quasiparticle recombination time in superconducting films, consisting of the standard electron-phonon interaction and a yet to be identified low temperature process, is studied for different densities of magnetic and nonmagnetic atoms. For both Ta and Al, implanted with Mn, Ta and Al, we observe an increase of the recombination rate. We conclude that the enhancement of recombination is not due to the magnetic moment, but arises from an enhancement of disorder.Comment: 4 pages, 4 figure

Effects of Different Production Systems on the Nutrient Density of Beef

As concerns regarding beef production systems on human health and animal welfare become more apparent, consumer interest in pasture-raised livestock has been steadily on the rise in the US. Such interest has increasingly led to questions about potential nutritional composition differences in beef from different production systems, such as grass-fed beef and grain-fed beef. The goal of this work was to conduct untargeted metabolomics analysis on a broad range of samples from the US beef supply chain to provide insight into how different finishing systems impact the beef metabolome and nutrient density. Here, we found that 191 out of 802 profiled compounds were different between grass-fed and grain-fed ribeye steaks (all, p \u3c 0.05), with phytochemicals, vitamins, lipid, and amino acid metabolites emerging as the most discriminatory metabolite classes. On average, phytochemicals were 1.7-fold higher in grass-fed beef compared to grain-fed beef (p \u3c 0.05) with considerable variation (4.4-fold) amongst individual farms, particularly within grass-fed beef systems. Alpha-tocopherol was 2-fold elevated in grass-fed beef, while nicotinamide was 1.3-fold elevated in grain-fed beef, respectively (p \u3c 0.05). We also observed that 4- hydroxy-nonenal-glutathione, a common marker of oxidative stress, was 2.7-fold elevated in grain-fed beef samples (p \u3c 0.05), with a 20-fold variation across individual farms. Future work will identify the source(s) of variation and best practices in beef systems to improve beef nutrient density and animal metabolic health

Surface-Soil Aggregation and Organic C and N Fractions Under Paired Grassland and Cropland Sites in the Southeastern USA

Grasslands are expected to deliver ecosystem services by sequestering soil organic C, improving soil health and water quality, and minimizing soil erosion. Data to support these ecosystem services from contemporary managed grasslands are relatively scant, and so on-farm measurements would help bolster assessment across more diverse environmental settings. This study was conducted to compare soil properties from paired landuse of croplands and grasslands in a diversity of Major Land Resource Areas – the Piedmont, Blue Ridge, and Blackland Prairie of the southeastern USA. Four separate pastures on three collaborating farms were sampled at depth of 0-10 cm. Four samples from a neighboring cropland field were paired with these pastures. A variety of soil chemical, physical, and biological properties were determined. Soil chemical properties were occasionally different between land management systems on one of the three farms, but few consistent differences occurred across farms. Dry-stable mean-weight diameter (MWD) was not different between paired land management systems, but water-stable MWD was dramatically reduced at all three locations with cropland compared with pasture. Soil stability index (water-stable MWD divided by dry-stable MWD) averaged 0.64 mm mm-1 under cropland and 0.91 mm mm-1 under pasture, suggesting that pastures had a highly stable soil surface that was resistant to erosion and likely contributed to high water infiltration. Soil organic C and N fractions (i.e., total, particulate, and mineralizable) were all significantly greater under pasture than under cropland, indicating that these pastures were indeed storing more C and N, and contributing to greater soil biological activity. This study provides evidence that well-managed grasslands can sequester soil organic C and N, improve soil surface stability conditions to foster water infiltration and reduced runoff, and may have important implications for habit development for soil-dwelling organisms

Integrating Plant Secondary Metabolites and Foraging Behavior to Enhance Animal Health in Ruminant Production Systems

Legumes and forbs contain bioactives or plant secondary compounds (PSC) with potential to enhance animal health through their antibiotic, antioxidant and immunomodulatory properties that are evident even at small dietary concentrations. In turn, ruminants can regulate their ingestion of PSC through behavioral mechanisms that allow for the efficient achievement of homeostasis. High concentrations of PSC lead to food avoidance, whereas lower content of PSC in the diet achieved through regulatory mechanisms of ingestion could promote medicinal and/or prophylactic effects in the animal and concomitant health benefits to milk and meat products. Under this context, we discuss the restructuring of rangelands and pasturelands through the strategic distribution of legume- and forb-rich patches in monotonous landscapes dominated by grasses, thus re-establishing their functionality. Such strategies can synergistically complement and provide new dimensions (prophylactic-medicinal, product quality) to the forage resources already available to livestock

Assessing risks of low-carbon transition pathways

Organisation and Governanc

Calpain- and talin-dependent control of microvascular pericyte contractility and cellular stiffness

Pericytes surround capillary endothelial cells and exert contractile forces modulating microvascular tone and endothelial growth. We previously described pericyte contractile phenotype to be Rho GTPase- and α-smooth muscle actin (αSMA)-dependent. However, mechanisms mediating adhesion-dependent shape changes and contractile force transduction remain largely equivocal. We now report that the neutral cysteine protease, calpain, modulates pericyte contractility and cellular stiffness via talin, an integrin-binding and F-actin associating protein. Digital imaging and quantitative analyses of living cells reveal significant perturbations in contractile force transduction detected via deformation of silicone substrata, as well as perturbations of mechanical stiffness in cellular contractile subdomains quantified via atomic force microscope (AFM)-enabled nanoindentation. Pericytes overexpressing GFP-tagged talin show significantly enhanced contractility (~ two-fold), which is mitigated when either the calpain-cleavage resistant mutant talin L432G or vinculin are expressed. Moreover, the cell-penetrating, calpain-specific inhibitor termed CALPASTAT reverses talin-enhanced, but not Rho GTP-dependent, contractility. Interestingly, our analysis revealed that CALPASTAT, but not its inactive mutant, alters contractile cell-driven substrata deformations while increasing mechanical stiffness of subcellular contractile regions of these pericytes. Altogether, our results reveal that calpain-dependent cleavage of talin modulates cell contractile dynamics, which in pericytes may prove instrumental in controlling normal capillary function or microvascular pathophysiology.National Science Foundation (U.S.) (CAREER Award)American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowshi

Hypothermic in situ perfusion of the porcine liver using Celsior or Ringer-lactate solution

BACKGROUND: Hypothermic perfusion (HP) of the liver is applied during total vascular exclusion (TVE) to reduce ischemic injury during liver resection. No studies have been performed comparing different perfusion solutions for HP. The aim of this experimental study was to compare Ringer-lactate solution (RL) with Celsior solution (Cs) for HP in a pig model of 60-min TVE. METHOD: Twenty pigs underwent 60-min TVE of the liver. Groups were TVE without HP (no-HP, n = 9), TVE with HP using RL (n = 6), and TVE with HP using Cs (n = 5). Blood and liver tissue samples were taken before TVE and during 24-h reperfusion. RESULTS: In the no-HP group, plasma aspartate aminotransferase values were significantly increased during reperfusion (p <0.05), while liver tissue pO(2) levels (p <0.01) were decreased when compared to the HP groups. After 24-h reperfusion, bile production and liver tissue glutathione content were significantly higher (p <0.05) in the Cs group (42.0 +/- 1.7 mL/h and 44.9 +/- 2.2 nmol/mg, respectively) as compared to the RL group (31.5 +/- 3.5 mL/h and 19.6 +/- 1.8 nmol/mg, respectively). CONCLUSION: The protective effect of HP during TVE was confirmed in this study. HP with Cs was more effective in reducing ischemic injury as compared to HP with R

Oxidative stress and antioxidants at biosurfaces: plants, skin, and respiratory tract surfaces.

Atmospheric pollutants represent an important source of oxidative and nitrosative stress to both terrestrial plants and to animals. The exposed biosurfaces of plants and animals are directly exposed to these pollutant stresses. Not surprisingly, living organisms have developed complex integrated extracellular and intracellular defense systems against stresses related to reactive oxygen and nitrogen species (ROS, RNS), including O3 and NO2. Plant and animal epithelial surfaces and respiratory tract surfaces contain antioxidants that would be expected to provide defense against environmental stress caused by ambient ROS and RNS, thus ameliorating their injurious effects on more delicate underlying cellular constituents. Parallelisms among these surfaces with regard to their antioxidant constituents and environmental oxidants are presented. The reactive substances at these biosurfaces not only represent an important protective system against oxidizing environments, but products of their reactions with ROS/RNS may also serve as biomarkers of environmental oxidative stress. Moreover, the reaction products may also induce injury to underlying cells or cause cell activation, resulting in production of proinflammatory substances including cytokines. In this review we discuss antioxidant defense systems against environmental toxins in plant cell wall/apoplastic fluids, dead keratinized cells/interstitial fluids of stratum corneum (the outermost skin layer), and mucus/respiratory tract lining fluids