Sputum Induction in Children Is Feasible and Useful in a Bustling General Hospital Practice

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The Jeroen Bosch Hospital funded this study.Peer reviewedPublisher PD

Dilatant normal faulting in jointed cohesive rocks: a physical model study

Dilatant faults often form in rocks containing pre-existing joints, but the effects of joints on fault segment linkage and fracture connectivity are not well understood. We present an analogue modeling study using cohesive powder with pre-formed joint sets in the upper layer, varying the angle between joints and a rigid basement fault. We analyze interpreted map-view photographs at maximum displacement for damage zone width, number of connected joints, number of secondary fractures, degree of segmentation and area fraction of massively dilatant fractures. Particle imaging velocimetry provides insight into the deformation history of the experiments and illustrates the localization pattern of fault segments. Results show that with increasing angle between joint-set and basement-fault strike the number of secondary fractures and the number of connected joints increase, while the area fraction of massively dilatant fractures shows only a minor increase. Models without pre-existing joints show far lower area fractions of massively dilatant fractures while forming distinctly more secondary fractures

Identification of amino acids in HIV-2 integrase involved in site-specific hydrolysis and alcoholysis of viral DNA termini

The human immunodeficiency virus integrase (HIV IN) protein cleaves two nucleotides off the 3′ end of viral DNA and subsequently integrates the viral DNA into target DNA. IN exposes a specific phosphodiester bond near the viral DNA end to nucleophilic attack by water or other nucleophiles, such as glycerol or the 3′ hydroxyl group of the viral DNA molecule itself. Wild-type IN has a preference for water as the nucleophile; we here describe a class of IN mutants that preferentially use the 3′ hydroxyl group of viral DNA as nucleophile. The amino acids that are altered in this class of mutants map near the putative active-site residues Asp-116 and Glu-152. These results support a model in which multiple amino acid side-chains are involved in presentation of the (soluble) nucleophile. IN is probably active as an oligomeric complex, in which the subunits have non-equivalent roles; we here report that nucleophile selection is determined by the subunit that supplies the active site.</p

Identification of amino acids in HIV-2 integrase involved in site-specific hydrolysis and alcoholysis of viral DNA termini

The human immunodeficiency virus integrase (HIV IN) protein cleaves two nucleotides off the 3′ end of viral DNA and subsequently integrates the viral DNA into target DNA. IN exposes a specific phosphodiester bond near the viral DNA end to nucleophilic attack by water or other nucleophiles, such as glycerol or the 3′ hydroxyl group of the viral DNA molecule itself. Wild-type IN has a preference for water as the nucleophile; we here describe a class of IN mutants that preferentially use the 3′ hydroxyl group of viral DNA as nucleophile. The amino acids that are altered in this class of mutants map near the putative active-site residues Asp-116 and Glu-152. These results support a model in which multiple amino acid side-chains are involved in presentation of the (soluble) nucleophile. IN is probably active as an oligomeric complex, in which the subunits have non-equivalent roles; we here report that nucleophile selection is determined by the subunit that supplies the active site.</p

Evolution of a highly dilatant fault zone in the grabens of Canyonlands National Park, Utah, USA - Integrating fieldwork, ground-penetrating radar and airborne imagery analysis

Abstract. The grabens of Canyonlands National Park are a young and active system of sub-parallel, arcuate grabens, whose evolution is the result of salt movement in the subsurface and a slight regional tilt of the faulted strata. We present results of ground-penetrating radar (GPR) surveys in combination with field observations and analysis of high-resolution airborne imagery. GPR data show intense faulting of the Quaternary sediments at the flat graben floors, implying a more complex fault structure than visible at the surface. Direct measurements of heave and throw at several locations to infer fault dips at depth, combined with observations of primary joint surfaces in the upper 100 m, suggest a highly dilatant fault geometry. Sinkholes observed in the field as well as in airborne imagery give insights in local dilatancy and show where water and sediments are transported underground. Based on correlations of paleosols observed in outcrops and GPR profiles, we argue that either the grabens in Canyonlands National Park are older than previously assumed or that sedimentation rates were much higher in the Pleistocene. This is the final version of the article. It first appeared from Copernicus Publications via http://dx.doi.org/10.5194/se-6-839-2015 The supplement related to this article is available online at http://dx.doi.org/10.5194/se-6-839-2015-supplemen

New Observations and Analysis of the Bright Semi-Detached Eclipsing Binary mu1 Sco

Using new and published photometric observations of mu1 Sco (HR 6247), spanning 70 years, a period of 1.4462700(5) days was determined. It was found that the epoch of primary minimum suggested by Shobbrook at HJD 2449534.178 requires an adjustment to HJD 2449534.17700(9) to align all the available photometric datasets. Using the resulting combined-data light-curve, radial velocities derived from IUE data and the modelling software PHOEBE, a new system solution for this binary was obtained. It appears that the secondary is close to, or just filling, its Roche-lobe.Comment: 4 figures, 6 tables, 9 pages, uses mn2e.sty, to be published in MNRA