Film documentary : nodding syndrome: an illness in the making

This film documentary is based on fifteen months of anthropological fieldwork in northern Uganda and shows the complexity of nodding syndrome and the many different narratives, explanatory models and coping styles regarding this affliction. In attempting to understand what nodding syndrome is and how it is experienced, we should look at the context in which meaning making takes place

Native detection of protein O-GlcNAcylation by gel electrophoresis

O-GlcNAcylation is an abundant and dynamic protein posttranslational modification (PTM), with crucial roles in metazoans. Studies of this modification are hampered by the lack of convenient methods for detecting native O-GlcNAcylation. Here, we describe a novel gel-based approach, Separation of O-GlcNAcylated Proteins by Polyacrylamide Gel Electrophoresis (SOPAGE), which enables detection of O-GlcNAc levels and dynamics

O-GlcNAcase contributes to cognitive function in Drosophila

Contains fulltext : 219183.pdf (publisher's version ) (Open Access

Acetazolamide-based fungal chitinase inhibitors

Chitin is an essential structural component of the fungal cell wall. Chitinases are thought to be important for fungal cell wall remodelling, and inhibition of these enzymes has been proposed as a potential strategy for development of novel anti-fungals. The fungal pathogen Aspergillus fumigatus possesses two distinct multi-gene chitinase families. Here we explore acetazolamide as a chemical scaffold for the inhibition of an A. fumigatus ‘plant-type’ chitinase. A co-crystal structure of AfChiA1 with acetazolamide was used to guide synthesis and screening of acetazolamide analogues that yielded SAR in agreement with these structural data. Although acetazolamide and its analogues are weak inhibitors of the enzyme, they have a high ligand efficiency and as such are interesting leads for future inhibitor development

Hepatocellular adenoma 'A new perspective'

Since the introduction and widespread use of abdominal imaging technologies there has been an increase in the frequency of detection of space-occupying lesions in the liver. In particular due to the use of ultrasound imaging which is frequently used because of its safety and non-invasive nature and its accessibility. Liver tumors that are found by incidence are defined as findings unrelated to the clinical indication for which the imaging examination was performed. To decide which of these tumors needs therapy further characterization with highly advanced imaging modalities (magnetic resonance imaging (MRI)) or needle biopsy is needed. Core needle biopsy is still accepted as the gold standard for diagnosing tumors in various organs. However, there is an increasing role for radiology in the diagnosis of focal liver tumors, especially by the use of state-ofthe- art MRI. Moreover, most hepatic tumors can be diagnosed with confidence by the use state-of-the-art MRI, which may have consequences for the role of needle biopsy during work-up of focal liver lesions